文章摘要
曾东,王益平,倪学勤,汪开毓,吴梦.几株益生菌对鲤前肠黏液的体外黏附特性[J].水产学报,2010,34(1):147~152
几株益生菌对鲤前肠黏液的体外黏附特性
The adhesion characteristics of 5 probiotic strains to the carp foregut mucus in vitro
投稿时间:2009-04-29  修订日期:2009-08-25
DOI:10.3724/SP.J.1231.2010.06447
中文关键词:   黏附;黏液;益生菌
英文关键词: lectin  receptor  mucus  carp  adhesion  interaction
基金项目:教育部长江学者和创新团队发展计划项目(IRT0555);四川省教委应用基础项目(07ZA065);教育部长江学者和创新团队发展计划项目(IRT0848)
作者单位E-mail
曾东 四川农业大学动物医学院动物微生态研究中心 zend@sicau.edu.cn 
王益平 四川农业大学动物科技学院水产养殖系  
倪学勤 四川农业大学动物医学院动物微生态研究中心  
汪开毓 四川农业大学动物医学院鱼病研究中心 kywang@sicau.edu.cn 
吴梦 四川农业大学动物医学院动物微生态研究中心  
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中文摘要:
      采用体外固定鲤前肠黏液,结合细菌同位素示踪的方法,对来源于鲤肠道的2株肠球菌和1株柠檬酸杆菌以及养殖水体的2株芽孢杆菌的细菌表面凝集素和黏液蛋白受体的化学组成、对病原菌附着鲤前肠黏液的影响等进行了研究。结果表明,5株菌经高碘酸钠和蛋白水解酶修饰后,高碘酸钠能极显著降低芽孢杆菌、肠球菌和柠檬酸杆菌的黏附率(P<0.01),而蛋白水解酶对多数菌株的影响不显著(P>0.05),推测细菌表面的凝集素主要为具糖蛋白性质的物质。黏液蛋白经蛋白水解酶处理后,部分菌株的附着数量显著降低(P<0.05),但经高碘酸钠处理后,5株菌的黏附率均显著上升(P<0.05),提示黏液蛋白上存在的5株细菌的特异性受体物质可能为蛋白质。益生菌通过排斥、竞争和取代作用能够显著降低部分病原菌的黏附率。取代作用对病原菌黏附的抑制效果最好,但具有菌株特异性。
英文摘要:
      Using immobilized mucosal model in vitro, combined with the isotope tracing method, the adhesion characteristics of Enterococci,Citrobacter isolated from carp intestines and Bacillus isolated from water to the carp foregut mucus were investigated. Results showed that after the modification with proteolytic enzymes or sodium metaperiodate, the relative adhesion percentages of all five bacteria were declined extremely significantly after they were treated with sodium metaperiodate(P<0.01), but it was affected less by the proteolytic enzymes treatment(P>0.05), indicating that main lectins existing on the cell surface of the five bacteria are glycoprotein compounds. After the modification with proteolytic enzymes or sodium metaperiodate of mucus, the relative adhesion percentages of part of five bacteria were declined significantly after the mucus was treated with proteolytic enzymes(P<0.05), but it was affected significantly by the sodium metaperiodate treatment(P<0.05), indicating that the special receptors of the microorganisms are protein compounds. According to the interactions such as exclusion, competition, and displacement, the five probiotic bacteria can reduce the relative adhesion percentages of part pathogens significantly. The inhibition of displacement to the pathogens was best, but the disparity was led by the different strain.
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