文章摘要
纪元,刘岩,宫庆礼.小鼠生物法和酶联免疫法(ELISA)定量监测沿海5省养殖河豚鱼中的河豚毒素(TTX)[J].水产学报,2010,34(4):589~597
小鼠生物法和酶联免疫法(ELISA)定量监测沿海5省养殖河豚鱼中的河豚毒素(TTX)
Tetrodotoxin quantitative monitoring of cultured pufferfish collected from five coastal provinces in China by mouse method and enzyme linked immunosorbent assay(ELISA)
投稿时间:2009-09-22  修订日期:2010-01-16
DOI:10.3724/SP.J.1231.2010.06618
中文关键词: 养殖河豚鱼  河豚毒素  小鼠法  酶联免疫检测法
英文关键词: cultured puffer fish  tetrodotoxin  mouse assay  enzyme linked immunosorbent assay(ELISA)
基金项目:国家“十一五”科技支撑计划子课题“河豚鱼毒素监测及控毒技术研究”(2006BAK02A07-3)
作者单位E-mail
纪元 中国海洋大学 jiyuan0728@163.com 
刘岩 中国海洋大学  
宫庆礼 中国海洋大学  
摘要点击次数: 2838
全文下载次数: 1715
中文摘要:
      为进一步扩大国内养殖河豚鱼消费市场提供依据,应用小鼠生物法监测了我国辽宁、河北、山东,江苏和福建4种养殖河豚鱼(红鳍东方鲀,菊黄东方鲀,双斑东方鲀和暗纹东方鲀4种组织(皮、肉、肝脏和性腺)中河豚毒素(TTX)含量及周年变化,并用酶联免疫法(ELISA)同步检测了山东红鳍东方鲀的皮,肉和肝脏各组及个别卵巢样品。结果4种养殖河豚鱼的皮,肉和肝脏每组平均含毒量均属无毒(小鼠生物法检测结果小于0.5 μg/g, ELISA检测结果小于0.8 μg/g)。卵巢发现一强毒例,精巢则全部无毒。通过监测,发现养殖河豚鱼组织器官的毒力呈现一定的周年变化。ELISA法与小鼠生物试验测得的结果相符合。养殖河豚鱼皮,肉和精巢认为可以考虑科学安全的利用,肝脏须谨慎对待,由于养殖河豚鱼上市规格较小,卵巢一般无食用意义,加工过程应注意不要污染可食部分。
英文摘要:
      In order to provide first hand scientific data and to utilize domestic cultured puffer fish safely, four tissues(skin, muscle,liver and gonad) of four cultured pufferfish species (Takifugu rubripes, Takifugu fasciatus, Takifugu flavidus, Takifugu bimaculatus) collected from five coastal provinces(Liaoning, Hebei, Shandong, Jiangsu and Fujian) in China were monitored quantitatively by mouse method after grouping and concentration. The skin, muscle and liver groups of T.rubripes from Shandong Province and several ovaries were measured by enzyme linked immunosorbent assay(ELISA) at same time. The results indicated that the average TTX content in each group of skin, muscle and liver of the four species specimens were ‘non toxic’ in both of the mouse assay (less than 0.5 μg/g) and ELISA (less than 0.8 μg/g), and all the testes have no detectable TTX . However, a moderately toxic ovary sample was still detected. The annual variation of the average TTX content in groups of the muscle, liver and skin of cultured pufferfish can be observed. The results of the mouse method and ELISA method have no significant difference. It is confirmed that the skin, muscle and testis of the collected cultured pufferfish can be used with safety. The livers of the four species of puffer fish could be safely consumed as a delicious and nutritive food. The market size of cultured pufferfish was small, but still toxic. It is necessary to prevent the edible parts contamination.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看