文章摘要
赵广泰,刘贤德,王志勇,蔡明夷,姚翠鸾.大黄鱼连续4代选育群体遗传多样性与遗传结构的微卫星分析[J].水产学报,2010,34(4):500~508
大黄鱼连续4代选育群体遗传多样性与遗传结构的微卫星分析
Genetic structure and genetic diversity analysis of four consecutive breeding generations of large yellow croaker (Pseudosciaena crocea) using microsatellite markers
投稿时间:2009-09-26  修订日期:2009-11-04
DOI:10.3724/SP.J.1231.2010.06624
中文关键词: 大黄鱼  微卫星  遗传多样性  遗传结构  选育群体
英文关键词: large yellow croaker (Pseudosciaena crocea)  microsatellite  genetic diversity  genetic structure  selective and breeding population
基金项目:国家“八六三”高技术研究发展计划(2006AA10A405);集美大学创新团队基金(2006A001);福建省青年人才项目(2007F3074)
作者单位E-mail
赵广泰 集美大学 zhaoguangtai1984@163.com 
刘贤德 集美大学  
王志勇 集美大学 zywang@jmu.eud.cn 
蔡明夷 集美大学  
姚翠鸾 集美大学  
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中文摘要:
      利用13个多态性微卫星位点分析了大黄鱼“官井洋优快01”品系F1到F4 4个选育世代的遗传结构与遗传多样性变化情况。结果显示,随着选育的进行,4个世代群体遗传多样性指标值渐次下降,F1到F4 13个微卫星位点的平均多态信息含量从0.638下降到0.524,平均等位基因数从5.462下降到4.308,平均观测杂合度从0.779下降到0.532,平均Shannon多样性指数从1.356下降为1.092。F1与其后各代遗传相似系数逐渐减小(从0.719 4到0.581 3),遗传距离逐渐增加,而相邻世代间的遗传相似性逐步升高,遗传分化指数(FST)渐次变小(F1~F2为0.0619,F2~F3为0.0511,F3~F4则为0.047 5)。随着选育的进行,微卫星位点LYC0002和LYC0054 等位基因频率有规律地发生变化,推测其可能与选育性状存在遗传上的相关。结果表明,经过连续4代的选育,部分不利基因遭到淘汰,选育群体的遗传基础逐步得到纯化,基因型逐渐趋向纯合、稳定,经进一步的选育可望获得较稳定的品系。
英文摘要:
      Thirteen microsatellite markers were used to analyze the genetic structure and genetic diversity of the breeding populations “Guanjingyang Youkuai 01” from F1 to F4. The results showed that the genetic diversity of the breeding population was decreasing, the average polymorphism information content (PIC) of the 13 microsatellite markers decreased from 0.638 to 0.524, the average allele number from 5.462 to 4.308, the average heterozygosity from 0.779 to 0.532, and the average Shannon’s gene diversity index from 1.356 to 1.092, respectively. The genetic identity between F1 and its descendant generations (F2, F3, F4) decreased (from 0.719 4 to 0.581 3) while the genetic distance increased. The genetic identity between the adjacent generations increased and the FST values decreased (0.061 9 in F1-F2; 0.051 1 in F2-F3; 0.047 5 in F3-F4). The allele frequency of the loci LYC0002 and LYC0054 changed regularly in the four breeding generations and they might correlate with the selected traits, which should be proved by further research. Our study suggested that the selective breeding work was efficient, some adverse genes were phased out, the hereditary basis of the population was getting pure and the genetic structure would be stable with the continuation of the breeding work.
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