文章摘要
徐晓津,徐斌,王军,苏永全,张之文,陈心.大黄鱼感染哈维氏弧菌后血液生化指标的变化及组织病理学观察[J].水产学报,2010,34(4):618~625
大黄鱼感染哈维氏弧菌后血液生化指标的变化及组织病理学观察
Study on blood chemistry indices and histopathology of Pseudosciaena crocea Artificially challenged with Vibrio harveyi
投稿时间:2009-10-22  修订日期:2009-12-17
DOI:10.3724/SP.J.1231.2010.06665
中文关键词: 大黄鱼  哈维氏弧菌  血液生化指标  组织病理
英文关键词: Pseudosciaena crocea  Vibrio harveyi  blood chemistry indice  histopathology
基金项目:国家“八六三”高技术研究发展计划(2006AA10A405);国家自然科学基金项目(40976096);福建省高校水产科学技术与食品安全重点实验室(集美大学)基金;集美大学博士启动金资助项目
作者单位E-mail
徐晓津 集美大学水产学院, 福建省高校水产科学技术与食品安全重点实验室 xxj@jmu.edu.cn 
徐斌 厦门大学海洋学系, 厦门大学亚热带海洋研究所  
王军 厦门大学海洋学系, 厦门大学亚热带海洋研究所  
苏永全 厦门大学海洋学系, 厦门大学亚热带海洋研究所  
张之文 厦门大学海洋学系, 厦门大学亚热带海洋研究所  
陈心 厦门大学海洋学系, 厦门大学亚热带海洋研究所  
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中文摘要:
      从患病大黄鱼病灶中分离出病原哈维氏弧菌,并人工回接感染大黄鱼,病鱼出现典型的皮肤溃疡症。测定大黄鱼血液各项指标:病鱼单核细胞、嗜中性粒细胞、血栓细胞百分率明显下降, 而淋巴细胞百分率增加,均呈极显著差异(P<0.01)。患病大黄鱼总蛋白为(12.8±3.95) g/L,对照组鱼为(19.4±4.33) g/L,病鱼球蛋白、甘油三脂、血糖含量也明显低于对照组(P<0.01)。但病鱼谷丙转氨酶为(37±5.11) IU/L,对照组为(28±5.54) IU/L;病鱼谷草转氨酶为(164±47.19) IU/L,对照组为(117±21.84) IU/L,均呈极显著差异(P<0.01)。研究结果说明,病鱼肝脏和肾脏组织受损,对患病大黄鱼进行病理组织学观察,结果发现病鱼病变组织头肾、肝出现不同程度的变性以及坏死。细胞超微病变观察发现,头肾肾小管上皮细胞变性、坏死。肝细胞胞浆内脂滴积累多。血清指标的测定可以作为大黄鱼哈维氏弧菌病细菌感染的指标。
英文摘要:
      The pathogen Virio harveyi was isolated from the focus of illed Pseudosciaena crocea was infected back to the healthy P.crocea artificially. The challenged fish indicated the typical ulcered syndrome. The blood chemistry parameters were tested.The challenged fish indicated significantly(P<0.01)lower percentages of monocytes,polymorphonuclear neutrophil granulocytes(PMN) and thrombocytes but higher percentages of leucocytes; it differed from the control fish. Significant decreases in total protein, globulin, triacyl glycerol and blood glucoe concentrations were found in the challenged fish compared to the control fish(P<0.01),with the total protein values at (12.8 ±3.95) g/L and (19.4±4.33) g/L, respectively. Significant increases of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)concentrations were found in the challenged fish compared to the healthy fish(P<0.01). The concentrations of ALT and AST were (37±5.11) IU/L and (164±47.19) IU/L in the challenged fish, while in the control fish were (28±5.54) IU/L and (117±21.84) IU/L. The results indicated liver and kidney damage, and disturbances in hepatic and osmoregulatory function of P.crocea.Observations by histopathology showed that head kidney and liver of the tested P.crocea were severely damaged and various denaturalization and necrosis. Denaturalization and necrosis emerged in the epithelial cell of renal tubule under electronic microscope. Fatty were accumulated in the cytoplasm of hepatic cells. The serum chemistry parameters determinations was a good inicator of bacterial infection of the challenged fish.
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