文章摘要
严骏骢,赵金良,李思发,郑德锋.鲢中国土著群体与海外移居群体遗传多样性的AFLP分析[J].水产学报,2010,34(5):673~679
鲢中国土著群体与海外移居群体遗传多样性的AFLP分析
Genetic diversity of Chinese native populations and overseas transplanted populations of silver carp by AFLP
投稿时间:2009-10-27  修订日期:2009-12-16
DOI:10.3724/SP.J.1231.2010.06674
中文关键词:   遗传多样性  AFLP  土著群体  移居群体
英文关键词: Hypophthalmichthys molitrix  genetic diversity  amplified fragment lemgth polymorphism(AFLP)  native and transplanted population
基金项目:国家自然科学基金重点项目(30630051);上海市重点学科建设项目资助(Y1101)
作者单位E-mail
严骏骢 上海海洋大学 yanjuncong@qq.com 
赵金良 上海海洋大学 jlzhao@shou.edu.cn 
李思发 上海海洋大学  
郑德锋 上海海洋大学  
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中文摘要:
      摘要:鲢原产东亚地区,是我国淡水渔业的最大种类。自上世纪60年代始,我国长江、珠江、黑龙江鲢自然群体资源急剧衰退;与此同时,鲢被广泛移植世界各地,并在一些地区形成了新的自然群体。本研究采用AFLP技术从长江群体内(邗江、老河)、中国长江、珠江、黑龙江群体间以及中国土著群体和海外移居群体(多瑙河、密西西比河)间三个层面上分析了鲢自然群体在世界范围内的遗传格局,结果表明:邗江、老河、珠江、黑龙江群体的Nei’s基因多样性(H)分别为0.0481±0.1151、0.0659±0.1333、0.0510±0.1155、0.0661±0.1364,多瑙河、密西西比河群体分别为0.0576±0.1250、0.0540±0.1221;国内土著群体遗传多样性(0.0729±0.1295)高于海外移居群体。AMOVA分析表明,群体间差异对群体总遗传变异的贡献率为8.14%,而群体内差异的贡献率为91.86%。长江石首与邗江群体间的遗传分化FST值为0.07011(p<0.01),长江、珠江、黑龙江群体间的FST值为0.07044(p<0.01),中国土著群体与海外移居群体间的FST值为0.04243(p<0.01),鲢在国内土著群体内、土著群体与海外群体间的不同层面上均表现分化显著。研究结果为进一步监测海内、外鲢群体的遗传变化趋势积累基础资料。
英文摘要:
      The genetic diversity of silver carp (Hypophthalmichthys molitrix) populations around the world was thoroughly evaluated by AFLP at three different levels: populations within Yangtze River(Hanjiang section, YZ HJ and Laohe section, YZ LH), among Chinese native populations(Yangtze River, YZ, Pearl River, PR overseas Amur River, AMU) and between Chinese native populations and overseas transplanted populations (Danube River, DAU and Mississippi River, MIS). The results showed Nei’s genetic diversity (H) of native populations was 0.048 1±0.115 1, 0.065 9±0.133 3, 0.051 0±0.115 5, 0.066 1±0.136 4 for YZ HJ, YZ LH, PR, AMU, respectively; while it was 0.057 6±0.125 0, 0.054 0±0.122 1 for the transplanted populations of DAU and MIS, respectively. The whole genetic diversity of the Chinese native populations(0.072 9±0.129 5) was higher than that of the transplanted populations. AMOVA showed the variance within population(91.86%) was larger than that of interpopulation(8.14%). The genteic divergence index within Yangtze River group, Chinese native groups, Chinese native groups and overseas groups was 0.070 1(P<0.01), 0.070 4(P<0.01) and 0.042 4(P<0.01), respective, which showed there are significant differences in three different group levels. These results would lay a base for long observation on trend of genetic variation of silver carp native populations and transplanted populations.
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