文章摘要
魏华,吴楠,沈竑,成永旭,吴婷婷.溴氰菊酯对克氏原螯虾的氧化胁迫效应[J].水产学报,2010,34(5):733~739
溴氰菊酯对克氏原螯虾的氧化胁迫效应
Oxidative stress of deltamethrin to the liver of crayfish Procambarus clarkii
投稿时间:2009-11-16  修订日期:2010-01-12
DOI:10.3724/SP.J.1231.2010.06709
中文关键词: 溴氰菊酯  克氏原螯虾  急性毒性  超氧化物歧化酶  过氧化氢酶  丙二醛
英文关键词: deltamethrin  crayfish (Procambarus clarkii)  acute toxity  SOD  CAT  MDA
基金项目:上海市科委攻关项目(08dz1206002)
作者单位部门
魏华 上海海洋大学 水产与生命学院
吴楠 上海海洋大学水产与生命学院 
沈竑 上海沐雨河蟹研究所 
成永旭 上海海洋大学水产与生命学院 
吴婷婷 上海海洋大学水产与生命学院 
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中文摘要:
      为了解溴氰菊酯对克氏原螯虾类的毒性及致毒机理,采用24 h换水式生物试验研究了溴氰菊酯对克氏原螯虾的96 h急性毒性,分光光度法检测了6、12、24和48 h 后0.01、0.02和0.04 μg/L溴氰菊酯对肝胰腺超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力和丙二醛(MDA)的含量等氧化胁迫相关指标的影响。结果表明,24、48和96 h的半致死浓度分别为0.156 0、0.099 3和0.056 2 μg/L,安全浓度为5.62 ng/L;在整个暴露过程中,溴氰菊酯各个处理组都引起了氧化胁迫相关指标的变化。SOD和CAT活力的变化趋势相同,都呈抑制-诱导-抑制的变化规律,MDA含量则一直高于对照组。暴露6 h后,0.01 μg/L浓度组MDA含量极显著高于对照组(P<0.01),0.04 μg/L浓度组MDA含量约为对照组的1.98倍(P<0.05);暴露12 h后,MDA仍保持较高水平,0.02 μg/L浓度组MDA含量约为对照组的1.76倍(P<0.05);暴露24 h后,各浓度组CAT活力分别比对照组上升了70.98%、73.05%和66.67%(P<0.01);暴露48 h后,0.01、0.02 μg/L浓度组的SOD活力分别下降了60.38%和45.60%(P<0.01);各指标变化没有明显的剂量相关效应。结果提示,溴氰菊酯对克氏原螯虾毒性极强,在48 h内可以通过氧化损伤途径对机体产生毒性作用。鉴于对溴氰菊酯的高度敏感特点,克氏原螯虾也可以被用作水环境中菊酯类农药污染有效的指示生物。
英文摘要:
      Deltamethrin (DM), one of the regular pyrethroid pesticides, have toxical effects on aquatic animals after entering the natural water due to human activities. As a large sized crustacean species, crayfish (Procambarus clarkii) is an important link of eco-system of the water. In order to understand the toxical effects and mechanism of DM on P.clarkii, an acute semi static toxic test was carried out, and the oxidative stress relative indicators (activities of superoxide dismutase (SOD), catalase (CAT) and concentrations of malondialdehyde (MDA) in hepatopancreas) were examined at intervals (0,6,12 and 24 h) after treated with DM (with the concentration of 0.01,0.02 and 0.04 μg/L) by spectrophotometry. LC50 values of DM for P.clarkii in 24, 48 and 96 h were 0. 156 0、0.099 3 and 0.056 2 μg/L, respectively; and its safe concentration was 5.62 ng/L. In all groups treated with DM, the oxidative stress relative indicators varied. The activities of SOD and CAT showed the similar variation during the treatments: inhibition induction inhibition , while the levels of MDA were higher in all treated groups than in the control group. After 6 h treated with DM, MDA level in the group of 0.01 μg/L was significantly higher than in the control group(P<0.01),1.98 times higher in the group of 0.04 μg/L than in the control group(P<0.05); After 12 h treated with DM, MDA level was 1.76 times higher in the group of 0.02 μg/L than in the control group(P<0.05).The activities of CAT of three treated group were 70.98%、73.05% and 66.67% respectively higher than the control group after 24 h DM treated(P<0.01); After 48 h treated with DM, SOD activities decreased by 60.38% and 45.60% in the group of 0.01,0.02 μg/L, respectively(P<0.01).The three different treated groups did not show apparent dose dependent effects. The results provide evidences that DM performed an extremely high toxical effect on P.clarkii and may play its adverse impact through an oxidative damage pathway within 48 h 〖WTBX〗P. clarkii〖WTB1〗 could be used as a bio indicator to the pyrethroid pesticides pollution in the water as it is highly sensitive to pyrethroids.
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