文章摘要
钟国防,钱曦,华雪铭,周洪琪.玉米蛋白粉替代鱼粉对暗纹东方鲀溶菌酶活性及c型溶菌酶mRNA表达的影响[J].水产学报,2010,34(7):1121~1128
玉米蛋白粉替代鱼粉对暗纹东方鲀溶菌酶活性及c型溶菌酶mRNA表达的影响
Effects of feeding with corn gluten meal on lysozyme activity and c-lysozyme gene expression in Fugu obscurus
投稿时间:2009-11-16  修订日期:2010-04-22
DOI:10.3724/SP.J.1231.2010.06711
中文关键词: 暗纹东方鲀  玉米蛋白粉  溶菌酶活性  溶菌酶基因mRNA表达
英文关键词: Fugu obscurus  corn gluten meal  lysozyme activity  lysozyme gene expression
基金项目:上海市科委重大科技攻关项目(06DZ19102,073205111);农业部水产种质资源与利用重点开放实验室开放课题(KFT2008-2)
作者单位E-mail
钟国防 上海海洋大学 gfzhong@shou.edu.cn 
钱曦 上海海洋大学  
华雪铭 上海海洋大学  
周洪琪 上海海洋大学  
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中文摘要:
      以初重为(41.26±1.09) g的暗纹东方鲀为实验鱼,配制5种玉米蛋白粉使用量为0%、5%、10%、15%、20%的实验饲料,玉米蛋白粉对饲料中鱼粉的替代水平分别为0%、7.4%、14.8%、22.2%和29.6%。用实验饲料饲养暗纹东方鲀60 d,研究玉米蛋白粉替代鱼粉对暗纹东方鲀肝胰脏、头肾和脾脏组织的溶菌酶活性及其c 型溶菌酶mRNA相对表达量的影响。结果表明,(1) 暗纹东方鲀肝胰脏、头肾和脾脏的溶菌酶比活力分别为9.14、42.12和40.49 U/mg prot,头肾和脾脏组织中c 型溶菌酶mRNA相对表达量分别是肝胰脏的3.76和3.24倍。(2) 玉米蛋白粉替代鱼粉显著影响暗纹东方鲀溶菌酶比活力。5%和10%组的肝胰脏和头肾组织溶菌酶比活力显著高于对照组(P<0.05),而15%和20%组肝胰脏和头肾组织溶菌酶比活力则显著低于对照组(P<0.05);5%和10%组的脾脏组织溶菌酶活力与对照组无显著性差异(P>0.05),而15%和20%组显著低于对照组和5%组(P<0.05)。(3) 玉米蛋白粉对暗纹东方鲀c型溶菌酶基因mRNA相对表达量有显著影响(P<0.05)。肝胰脏组织中5%和10%组表达量显著高于对照组,而15%和20%组则显著低于对照;头肾组织中5%组与对照组无显著性差异,10%组显著高于对照组,而15%和20%组则显著低于对照组。脾脏组织中5%、10%组表达量与对照组无显著性差异,而15%和20%组则显著低于对照组。综上所述,适量使用玉米蛋白粉能提高暗纹东方鲀溶菌酶比活力及其c 型溶菌酶基因mRNA相对表达量,两者都在玉米蛋白粉使用量为10%时达到峰值。
英文摘要:
      To examine the effects of corn gluten meal on lysozyme activity and c-lysozyme gene expression in Fugu obscurus,the fish were fed with diets containing 0%,5%,10%,15% and 20% corn gluten meal in replacement of 0%,7.4%,14.8%,22.2% and 29.6% of conventional fish meal for 60 days,respectively.Lysozyme activities and mRNA levels in the hepatopancreas,head kidney and spleen were examined.The results show that:(1) At the beginning of the experiment,the lysozyme activities of the hepatopancreas,head kidney and spleen were 9.14 U/mg protein,42.12 U/mg protein and 40.49 U/mg protein,respectively.The c-lysozyme mRNA relative expression levels of head kidney and spleen were 3.76 and 3.24 times of the hepatopancreas.(2) Different amounts of corn gluten meal in the diets exerted different effects on the lysozyme activities.The lysozyme activities of hepatopancreas in the 15% and 20% trial groups were significantly lower than that of the control group,and they significantly increased in the 5% and 10% trial groups.In the head kidney,lysozyme activities were significantly enhanced in the 10% trial group,but they significantly reduced in the 15% and 20% trial groups.In the spleen,there were not significant changes on the enzyme activities in the 5% and 10% trial groups,but in the 15% and 20% trial groups,the enzyme activities significantly reduced.(3) Different amounts of corn gluten meals in the diets also resulted in significant changes in c-lysozyme mRNA levels in tissues.In comparison with the control group,the c-lysozyme mRNA expression levels of hepatopancreas significantly reduced in the 15% and 20% trial groups,although they significantly increased in the 5% and 10% trial groups.In the spleen,there were not significant changes on the c-lysozyme mRNA expression levels in the 5% and 10% trial group,but they were significantly lower in the 15% and 20% trial groups than that of the control group.In the head kidney, c-lysozyme mRNA expression levels were significantly enhanced in the 10% trial group,but they significantly reduced in the 15% and 20% trial groups.In a word,the enzyme activities and c-lysozyme mRNA expression levels were peaked in the 10% corn gluten meal group.
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