文章摘要
高国强,常玉梅,匡友谊,梁利群.利用OneMap软件构建鲤遗传连锁图谱[J].水产学报,2010,34(5):649~655
利用OneMap软件构建鲤遗传连锁图谱
The contruction of genetic linkage map of common carp (Cyprinus carpio L.)using OneMap software
投稿时间:2009-12-15  修订日期:2010-03-06
DOI:10.3724/SP.J.1231.2010.06742
中文关键词:   遗传连锁图谱  荧光扩增片段长度多态性;OneMap
英文关键词: common carp (Cyprinus carpio)  genetic linkage map  fluorescent amplification fragment length polymorphism(fAFLP)  OneMap
基金项目:国家“九七三”计划资助项目(2004CB117400); 农业部水产生物技术重点开放实验室(2009NYBZS-05); 中央级公益性科研院所基本科研业务费专项资金项目(2009HSYZX-SJ-05)
作者单位E-mail
高国强 中国水产科学研究院黑龙江水产研究所 gqgao2008@126.com 
常玉梅 中国水产科学研究院黑龙江水产研究所  
匡友谊 中国水产科学研究院黑龙江水产研究所  
梁利群 中国水产科学研究院黑龙江水产研究所  
摘要点击次数: 2865
全文下载次数: 2068
中文摘要:
      首次使用R环境中的OneMap软件包,以荷包红鲤抗寒品系()和云南大头鲤(♀)为祖父母本所培育的110个F2个体为作图群体,以荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism, fAFLP) 为主要分子标记,采用远交全同胞家系模型构建鲤的遗传连锁图谱。结果显示,110个F2个体中共产生1 513个清晰的fAFLP标记,其中多态性标记911个;另开发多态性的EST标记12个,最后总计923个标记用于构建遗传连锁图谱;采用OneMap软件包构建的遗传图谱含有238个fAFLP标记和8个EST标记分布在50个连锁群上,总图距为2 876.64 cM,标记间平均间距为14.68 cM,图谱覆盖率为66.56%。
英文摘要:
      The common carp (Cyprinus carpio L.) is the most extensively cultured fish in the world, as well as in China. Construction of genetic linkage map of common carp is necessary to increase the efficiency of selective breeding, especially complicated traits. So, it is the basis of keeping on the genetic study of a species with construction of full genetic map and location of quantitative trait. In this study,a genetic linkage map has been constructed for the common carp (C.carpio L.) using the mode of outbreeding full sib family in OneMap software firstly. The segregation of 923 polymorphic markers, including 911 fAFLP (fluorescent amplification fragment length polymorphism, fAFLP) markers and 12 gene markers were studied in a panel with 110 F2 individuals derived from a single hybrid F1, which was produced by crossing of red purse carp(C.carpio var.wuyuanensis)and big head carp (C.pellegrini Tchang). The results showed that the framework of genetic linkage map consisted of 238 fAFLP markers and 8 EST markers in 50 linkage groups that ranged in size from 2.01 cM to 437.46 cM and consisted of between 2 and 48 markers. The total linkage distance spanned by these markers was 2 876.64 cM with an average of 14.68 cM for the whole framework map, and the map coverage is 66.56%.This genetic linkage map is an improvement of further quantitative traits loci (QTL), fine mapping, marker assisted selection(MAS) linked to important economic traits and comparative genome mapping in common carp.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看