文章摘要
闫喜武,张跃环,孙焕强,霍忠明,孙欣,杨凤,张国范.菲律宾蛤仔两道红与白斑马品系的三元杂交[J].水产学报,2010,34(8):1190~1197
菲律宾蛤仔两道红与白斑马品系的三元杂交
Three way crosses between two band red and white zebra strains of Manila clam,Ruditapes philippinarum
投稿时间:2010-01-16  修订日期:2010-05-21
DOI:10.3724/SP.J.1231.2010.06802
中文关键词: 菲律宾蛤仔  两道红  白斑马  三元杂交
英文关键词: Ruditapes philippinrum  two-band red strains  white zebra strains  three way crosses
基金项目:国家“八六三”高技术研究发展计划(2006AA10A410);国家“十一五”规划项目(2006BAD09A09);辽宁省重大科研项目(990387);辽宁省教育厅创新团队项目(2007T104);辽宁省科技特派团项目(2008203005)
作者单位E-mail
闫喜武 大连水产学院 yanxiwu2002@163.com 
张跃环 大连海洋大学 kilroy9269@163.com 
孙焕强 大连海洋大学生命科学与技术学院  
霍忠明 大连海洋大学  
孙欣 大连海洋大学  
杨凤 大连海洋大学  
张国范 中国科学院海洋研究所海洋生物中心  
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中文摘要:
      为了提高菲律宾蛤仔壳色品系的生产性能,于2009年8月,以具有较快生长速度的两道红(R)F2和具有显著杂种优势的白斑马(WZ)F2为试验材料,开展了两个蛤仔品系的三元杂交。其中白斑马品系生长快,抗逆性强,是由生长最快的珍珠白(W)和抗逆性最强的斑马蛤(Z)杂交产生的二元品系。试验由RR(♀R×)、RWZ(♀R×)、WZR(♀WZ×R)、WZWZ(♀WZ×WZ)4个试验组组成。获得了三元正反交组合RWZ、WZR,比较了各试验组子代在不同阶段生长、存活的杂种优势并分析了壳色遗传机制。结果表明,正反交组的单亲杂种优势具有明显的不对称性,正交组RWZ的生长与存活性状得到了明显的改良;WZR的存活性状得到了一定程度上的改良。从生长上看,在浮游期,RWZ、WZR的单亲杂种优势分别为+1.70、-2.92;双亲生长优势为-0.68,主要受到卵源与配对策略交互作用的影响,其次为母本效应;在稚贝培育期,RWZ的单亲生长优势为+9.71,WZR单亲生长劣势为-6.57,总体上尚未表现出双亲生长优势,其大小仅为+0.90。从存活上看,RWZ、WZR在浮游期的单亲存活优势分别为+4.47、+3.05;双亲存活优势为+3.60,主要受到配对策略的影响,其次为母本效应;正反交组在稚贝阶段的单亲杂种优势分别为+13.09、+7.30;双亲存活优势为+9.00。R×R、R×WZ、WZ×R、WZ×WZ子代的壳色分别表现为两道红、两道红白斑马、两道红白斑马、白斑马;白斑马自交后代仍然为白斑马,未出现壳色分离,且三元正反交的子代壳色表现一致,说明壳色为非伴性遗传。
英文摘要:
      In order to improve the phenotypic character of Manila clam Ruditapes philippinarum,three way crosses between Two-band Red(R:fast growth)and White Zebra strains(WZ:obvious heterosis)were conducted in August 2009.The generation F2 of White Zebra strain was from two way crosses between Pearl White and Zebra strains.The experiments consisted of two self fertilized groups(RR,WZWZ)and two hybrid groups(RWZ,WZR).Then the growth,survival,heterosis and shell color genetic mechanism were investigated in the study.The results showed that the single parental heterosis was asymmetrical between two reciprocal crosses,and the growth and survival were clearly improved in RWZ group,whereas only survival heterosis was observed in another group.Considering growth,the value of mid parental heterosis was -0.68,and that of single parental heterosis was +1.70 for RWZ,and -2.92 for WZR,respectively.It was mainly affected by the interaction between egg origin and mating strategy,next is maternal effect during planktonic stage.The value of mid parental heterosis was only +0.90,and that of single parental heterosis was +9.71 for RWZ,and -6.57 for WZR during juvenile stage.Considering survival,the value of mid parental heterosis was +3.60,and that of single parental heterosis was +4.47 for RWZ,and +3.05 for WZR,respectively.It was mainly affected by the mating strategy,next that is maternal effect during planktonic stage.The value of mid parental heterosis was +9.00,and that of single parental heterosis was +13.09 for RWZ,and +7.30 for WZR during juvenile stage.The shell color of generation for R×R,R×WZ,WZ×R,WZ×WZ were two band red,two band red with white zebra,two band red with white zebra and white zebra,respectively.The generation of white zebra was still white zebra on the outer shell color,that is,no shell color separated for two way hybrids.These results indicated that coloring was linked to sex and was not of sex-linked inheritance.
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