文章摘要
张俊玲,施志仪,程琦,贾亮.牙鲆变态中两种HSP90基因的不同表达及其与甲状腺激素的关系[J].水产学报,2010,34(10):1478~1485
牙鲆变态中两种HSP90基因的不同表达及其与甲状腺激素的关系
Differential expression of HSP90 genes and its relation with thyroid hormone during metamorphosis in Japanese flounder(Paralichthys olivaceus)
投稿时间:2010-02-25  修订日期:2010-04-19
DOI:10.3724/SP.J.1231.2010.06841
中文关键词: 牙鲆  变态发育  热休克蛋白90  基因表达  甲状腺激素  硫脲  荧光定量RT-PCR
英文关键词: Paralichthys olivaceus  metamorphosis  HSP90  gene expression  thyroid hormone  thiourea  fluorescent quantitative RT-PCR
基金项目:国家自然科学基金项目(30571420);上海市重点学科水生生物学建设项目(S30701);上海海洋大学校青年基金项目(05-151)
作者单位E-mail
张俊玲 上海海洋大学水产与生命学院 jlzhang@shou.edu.cn 
施志仪 上海海洋大学  
程琦 上海海洋大学  
贾亮 上海海洋大学  
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中文摘要:
      热休克蛋白90(heat shock protein90,HSP90)是细胞内主要的伴侣蛋白,在激素信号转导、细胞分化、细胞增殖与凋亡、形态发生与进化、变态发育及应激防御等多重调节路径中发挥关键作用。采用实时荧光定量RT-PCR技术检测了牙鲆两种HSP90基因在仔鱼发育和在成年组织中的表达变化。结果表明,HSP90α在成鱼骨骼肌、肠和胃中有较高的表达,而HSP90β在成鱼脑、脾脏和肾脏中有较高的表达。HSP90α mRNA水平在仔鱼变态期间迅速增加,至变态高峰G期达到最高水平;相反,HSP90β转录在仔鱼整个变态期间变化不是特别明显。鉴于甲状腺激素(thyroid hormone,TH)在牙鲆变态中的重要作用,还运用外源的TH及硫脲(thiourea,TU)处理牙鲆仔鱼来确定TH对HSP90基因转录的调节。与未处理组相比,HSP90α转录在TH处理8 d和13 d的仔鱼中显著增加,而在TU处理仔鱼中明显减少;但HSP90β mRNA水平不受药物处理影响。从上述结果分析,牙鲆中HSP90α的转录很可能被甲状腺激素上调,而且其在牙鲆的变态发育中发挥了重要作用。
英文摘要:
      HSP90 proteins are major chaperones that play pivotal roles in controlling multiple regulatory pathways such as hormone signaling,cell differentiation,cell proliferation and apoptosis,morphogenesis and morphological evolution,metamorphosis and stress defense.Using real time fluorescent quantitative RT-PCR approach,expression profiles of HSP90 genes were examined during larval development and in adult tissues in Japanese flounder,Paralichthys olivaceus.In adult tissues,HSP90α was mainly expressed with higher transcript levels in skeletal muscle,intestine and stomach; but HSP90β exhibited higher mRNA levels in brain,spleen and kidney.During metamorphosis,HSP90α transcript increased distinctly with the highest mRNA levels at the metamorphosis climax; whereas HSP90β mRNA levels did not change.Due to the important role of thyroid hormone(TH)for the flounder metamorphosis,the transcriptional regulation of HSP90 genes by TH was evaluated using exogenous TH and thiourea(TU)treatment.Compared with untreated control,HSP90α transcript increased significantly in larvae sampled 8 and 13 days after TH treatment,and TU-treated larvae exhibited lower HSP90α mRNA levels.In contrast,the transcript levels of HSP90β did not vary after TH and TU treatment.Overall,these results demonstrate that HSP90α transcription is up regulated by thyroid hormone and plays an important role during metamorphosis in Japanese flounder.
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