文章摘要
杨丽丽,张晶,方展强.雌二醇、壬基酚、多氯联苯、镉和锌及其混合物对唐鱼的雌激素效应比较[J].水产学报,2011,35(6):838~845
雌二醇、壬基酚、多氯联苯、镉和锌及其混合物对唐鱼的雌激素效应比较
Comparative study of estrogenic effects of estradiol,nonylphenol,polychlorinated biphenyls,cadmium,zinc and its mixtures on Tanichthys albonubes
投稿时间:2011-03-06  修订日期:2011-04-20
DOI:10.3724/SP.J.1231
中文关键词: 唐鱼  内分泌干扰物  卵黄蛋白原(Vtg)  Native-PAGE电泳
英文关键词: Tanichthys albonubes  environmental endocrine disrupting chemicals(EDCs)  vitellogenin(Vtg)  Native polyacrylamide gel electrophoresis(Native-PAGE)
基金项目:广东省科技计划项目(2009B030600006);国家科技支撑项目(2009BADB2B0401-02)
作者单位
杨丽丽 华南师范大学生命科学学院,广东省高等学校生态与环境重点实验室 
张晶 华南师范大学生命科学学院,广东省高等学校生态与环境重点实验室 
方展强 华南师范大学生命科学学院,广东省高等学校生态与环境重点实验室 
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中文摘要:
      以唐鱼卵黄蛋白原(Vtg)为检测指标,利用Native-PAGE电泳、唐鱼Vtg特异性脂蛋白染色和间接酶联免疫吸附(ELISA)技术分别检测了17β-雌二醇(E2)、壬基酚(NP)、多氯联苯(PCBs)、Cd2+、Zn2+及其混合物对唐鱼的雌激素效应。Native-PAGE结果显示,10 μg/L、50 μg/L E2水体暴露7、14 d 均诱导雄性唐鱼体内合成了Vtg,E2对唐鱼的雌激素效应具有时间累积效应,并随浓度升高而增强。而ELISA检测结果则显示0.1、0.5、1.0 μg/L E2 7、14 d和200、300 μg/L NP 14 d暴露使唐鱼合成了Vtg。结果表明,NP、Cd2+、Zn2+与不同浓度的E2联合雌激素效应不同于单一毒物的雌激素效应,NP与E2表现为协同促进作用。并讨论了环境雌激素的联合作用机制相关问题。
英文摘要:
      Environmental endocrine disrupting chemicals(EDCs) can affect the normal endocrine system of organism.Its huge threat to the environment and the health of human beings arouses international attention,which has been the hot topic in environmental research.Vitellogenin(Vtg) production in male and juvenile fish can provide a sensitive biomarker of reproductive disruption to indicate and estimate estrogenicity of EDCs.It has been proved that the vitellogenin of Tanichthys albonubes is a perfect biomarker to be used to monitor EDCs in environment.The objective of the present study was to detect the estrogenic responses of estradiol-17 beta(E2),nonylphenol(NP),polychlorinated biphenyls(PCBs),Cd2+,Zn2+ and its mixture in T.albonubes.Native-polyacrylamide gel electrophoresis(Native-PAGE),lipo-staining method,indirect enzyme-linked immunosorbent assay(ELISA) were used to analyze the whole body homogenate from treated male T.albonubes.The results of Native-PAGE showed that after exposure to 10 μg/L E2,50 μg/L E2 for 7 days or 14 days,the male T.albonubes could synthesize Vtg.It also showed that the more time,the more vitellogenin and the higher the concentration of E2,the more Vtg.The results of ELISA showed that the male T.albonubes could be induced to synthesize Vtg after exposure to 0.1,0.5,1.0 μg/L E2 for 7,14 days or exposure to 200,300 μg/L NP for 14 days.The combined effects of NP,PCB,Cd2+ or Zn2+together with different level E2 were dissimilar with their individual effects.The interaction of NP and E2 was synergism action.This article also discussed the combined effects of environmental estrogen mechanism.
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