文章摘要
王瑞芳,庄平,冯广朋,章龙珍,黄晓荣,贾小燕.盐度升高对中华绒螯蟹几种非特异性免疫因子的影响[J].水产学报,2012,36(4):546~552
盐度升高对中华绒螯蟹几种非特异性免疫因子的影响
Changes in non-specific immune parameters of mature Chinese mitten crab (Eriocheir sinensis) in response to elevated salinities
投稿时间:2011-08-27  修订日期:2011-12-12
DOI:10.3724/SP.J.1231.2012.27707
中文关键词: 中华绒螯蟹  盐度  免疫酶  氧合血蓝蛋白  性别差异  生殖洄游
英文关键词: Eriocheir sinensis  salinity  immune enzyme  oxyhemocyanin  sex difference  reproductive migration
基金项目:国家公益性行业(农业)科研专项(200903048-07, 201203065); 中央级公益性科研院所基本科研业务费专项(东海水产研究所2011M08); 国家“九七三”计划(2010CB429005); 长江口重要渔业资源养护与利用关键技术集成与示范(201203065)
作者单位
王瑞芳 华东师范大学生命科学学院 
庄平 中国水产科学研究院东海水产研究所, 农业部海洋与河口渔业资源及生态重点开放实验室 
冯广朋 中国水产科学研究院东海水产研究所, 农业部海洋与河口渔业资源及生态重点开放实验室 
章龙珍 中国水产科学研究院东海水产研究所, 农业部海洋与河口渔业资源及生态重点开放实验室 
黄晓荣 中国水产科学研究院东海水产研究所, 农业部海洋与河口渔业资源及生态重点开放实验室 
贾小燕 中国水产科学研究院东海水产研究所, 农业部海洋与河口渔业资源及生态重点开放实验室 
摘要点击次数: 2107
全文下载次数: 1885
中文摘要:
      采用逐步增盐法, 结合生物酶学测定, 研究了从淡水升高盐度到 7、14、21、28和35对二秋龄雌、雄中华绒螯蟹血清总蛋白、氧合血蓝蛋白含量及碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)、酚氧化酶(PO)活力的影响。结果显示, 随着盐度的升高, 中华绒螯蟹的血清总蛋白、氧合血蓝蛋白含量逐渐降低; AKP活性先略升高, 而后逐渐降低; 与雄蟹的PO活力逐渐降低相比, 雌蟹的PO活力先降低, 当盐度高于21后维持稳定水平; SOD活性先略降低, 盐度高于21被激活。除SOD外, 雌蟹血清各测定指标均显著高于雄蟹(P<0.05)。研究结果表明, 盐度接近或超过28将显著影响中华绒螯蟹3种免疫酶的活性及总蛋白和氧合血蓝蛋白的含量, 进而影响其免疫防御能力。
英文摘要:
      Mature Chinese mitten crabs, Eriocheir sinensis, are exposed to brackish water or coastal waters as an obligatory part during adult reproductive migration. In this study, we investigated the changes in serum total protein and oxyhemocyanin contents, alkaline phosphatase (AKP), superoxide dismutase (SOD) and phenoloxidase (PO) activities in both mature male and female E. when in freshwater and after step-wise acclimation to the following salinities: 7, 14, 21, 28, 35. Increasing salinities caused a gradual decrease in serum total protein and oxyhemocyanin contents. Serum AKP activity was elevated slightly at 7, thereafter it dropped slowly by the end of experiment. Serum SOD activity showed a slight decrease after increase of salinity until 21, it was activated above 21. Serum PO activity decreased gradually with the increase of salinity in males; however, it maintained a stable level above 21 in females. All tested parameters in serum except for SOD were significantly higher in females than those in males. It is suggested that elevated salinity induced a decrease in serum total protein and oxyhemocyanin contents. Serum immune enzyme activities in mature E. sinensis will be affected when the salinity was near to or above 28, which could exert a negative effect on reproduction. Further studies are needed to understand the biochemical adaptation of mature E. sinensis, during their reproductive migration acclimation to elevated salinities. Sex differences should not be ignored.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看