文章摘要
范飞龙,游伟伟,战欣,骆轩,陈楠,柯才焕.不同壳色杂色鲍F1家系的早期生长及遗传结构比较[J].水产学报,2012,36(9):1349~1357
不同壳色杂色鲍F1家系的早期生长及遗传结构比较
Comparison of growth traits at early stages and genetic diversity of F1 among different shell color lines of the small abalone(Haliotis diversicolor)
投稿时间:2011-11-22  修订日期:2012-06-03
DOI:DOI:10.3724/SP.J.1231.2012.27841
中文关键词: 杂色鲍  壳色  生长  遗传结构  家系
英文关键词: Haliotis diversicolor  shell color  growth  genetic diversity  family
基金项目:现代农业产业技术体系建设专项(nycytx-47);福建省自然科学基金项目(2011J05095);科技部农转资金项目(2010GB23600665)
作者单位
范飞龙 厦门大学海洋与地球学院,近海海洋环境国家重点实验室 
游伟伟 厦门大学海洋与地球学院,近海海洋环境国家重点实验室 
战欣 厦门大学海洋与地球学院,近海海洋环境国家重点实验室 
骆轩 厦门大学海洋与地球学院,近海海洋环境国家重点实验室 
陈楠 厦门大学海洋与地球学院,近海海洋环境国家重点实验室 
柯才焕 厦门大学海洋与地球学院,近海海洋环境国家重点实验室 
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中文摘要:
      在“东优1号”杂色鲍养殖群体中,实验发现了少量的黄壳色变异个体,为得到具有优良经济性状的新品种,实验通过自交和杂交的方法分别建立了正常壳色♀×正常壳色♂(BB)、黄壳色♀×黄壳色♂)〗(YY)、黄壳色♀×正常壳色♂(YB)和正常壳色♀×黄壳色♂(BY)4组家系,对各家系的卵黄径、受精率、孵化率、变态率、存活率和生长性状等生物学参数进行了比较,分析了杂交家系的杂种优势,并利用16个微卫星标记比较了4个家系的遗传差异。结果发现,黄壳色家系的卵黄径显著大于正常壳色家系的卵黄径(P<0.05),各家系的受精率、变态率和存活率没有显著的差异(P>0.05),幼体壳长和壳宽在不同的发育时间里,表现的杂种优势不稳定,在90日龄时,4个家系生长差异不显著。家系BB的遗传多样性最低,遗传距离(0.198 6~0.457 3)和遗传相似性系数(0.632 9~0.897 2)分析表明,BB和YY家系间的遗传距离最大(0.457 3),遗传相似性系数最低(0.632 9),杂色鲍黄壳色的自交家系与正常壳色的自交家系产生了遗传分化。
英文摘要:
      A small amount of yellow shell color of small abalone were found in the stock of “Dongyou No.1”.In order to understand the relationship between shell color and growth traits,four different color lines of Haliotis diversicolor including BB(normal♀× normal♂),YY(yellow♀× yellow♂),YB(yellow♀× normal♂)and BY(normal♀× yellow♂)were established by inbreeding and crossbreeding.The biological parameters of egg dismeter,fertilization rate,hatching rate,metamorphosis rate,survival rate and growth traits among different shell color lines were studied.Genetic diversity and genetic structure for the four lines were compared using 16 microsatellite markers.The results showed that yolk diameter of yellow shell color line was significantly larger than that of normal shell color line(P<0.05).Egg diameter,fertilization rate,hatching rate,metamorphosis rate and survival rate showed no significant differences(P>0.05)among BB,YY,YB and BY lines.At 90 days after fertilization,the growth of shell length and width among the four lines showed no significant differences(P>0.05).The results also showed that BB line had the lowest genetic diversity.In addition,Nei’s genetic identity(0.632 9-0.957 1)and genetic distance(0.043 9-0.457 3)indicated that genetic identity between BB and YY(0.632 9)was the lowest and genetic distance between them was the farthest(0.457 3).The results indicated genetic differentiation exists between normal and yellow color individuals.The present study provides basic data and theoretical basis for selective breeding of the small abalone using shell color as a candidate marker.
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