文章摘要
马 朋,刘 萍,李 健.脊尾白虾3个野生群体ITS1序列分析及其亲缘关系分析[J].水产学报,2012,36(8):1185~1192
脊尾白虾3个野生群体ITS1序列分析及其亲缘关系分析
The genetic diversity and phylogenetic analysis of ITS1 in mitochondrial DNA of three populations of Exopalaemon carinicauda
投稿时间:2012-01-04  修订日期:2012-02-26
DOI:DOI:10.3724/SP.J.1231.2012.27924
中文关键词: 脊尾白虾  ITS1  遗传多样性  系统进化
英文关键词:  Exopalaemon carinicauda,  ITS1,  genetics diversity,  Pphylogenetic
基金项目:国家“八六三“高技术研究发展计划项目资助(, 2012AA10A409)号; 国家虾产业技术体系(CARS-47)和; 山东省科技发展计划项目(2011GHY11526)
作者单位
马 朋 中国水产科学研究院黄海水产研究所 
刘 萍 中国水产科学研究院黄海水产研究所 
李 健 中国水产科学研究院黄海水产研究所 
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中文摘要:
      对脊尾白虾的莱州湾、海洲湾、象山湾3个野生群体共计62个个体的核糖体RNA转录单元内间隔区ITS1基因片段进行克隆和测序, 对序列特点进行分析, 并结合GenBank数据库中已有的长臂虾亚科ITS1同源序列虾类进行系统分析。结果显示, 脊尾白虾的ITS1序列具有长度多态性, 其长度为345~384 bp, 62条序列GC的平均含量显著高于AT含量; 共检测到79个变异位点, 39种单倍型, 多态位点比例为21.7%; 海洲湾群体遗传多样性指数最高, 象山湾群体次之, 莱州湾群体最低。在脊尾白虾ITS1序列中发现微卫星序列共有8处, 重复序列类型为(GC)n、(AG) n、(GGC) n、(GGA) n、 (AT) n、(GA) n, 以(GA) n类型最多。AMOVA分析结果显示3个群体间的遗传分化较弱或只有中度分化。另外用MEGA4.0软件中的NJ法构建分子进化树, 探讨长臂虾亚科几个种的系统进化关系, 系统树显示同种的不同个体、同属的不同种聚在一起, 与形态学的分类吻合。
英文摘要:
      The ribosomal DNA internal transcribed spacer 1 (ITS1) of three wild populations of Exopalaemon carinicauda was cloned and sequenced. The phylogenetic relationship among Palaemoninae was discussed using ITS1 from GenBank database. The results show that the length of the ITS1 ranged from 345 to 384 bp. The mean contents of GC were higher than AT;79 variation sites and 39 haplotypes were obtained in ITS1; Nucleotide diversity and average number of nucleotide diferences were calculated to analyze the gene sequence variation. Eight kinds of microsatellites were found in E. carinicauda. The type of (GA) n was rich in ITS1. AMOVA analysis results show that the genetic differentiation of the three groups was weak or only moderately differentiated.The molecular phylogenetic tree was constructed with NJ method using software MEGA 4.0 to study several species of the Palaemoninae molecular phylogeny. According to the NJ tree, different individuals of the same species, different species of the same genera were grouped together, in consistence with the morphological taxonomy.
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