文章摘要
孙田田,苏永全,洪婧妮,邬阳,张曼,毛勇.真蛸热休克蛋白90基因(HSP90)的克隆及表达[J].水产学报,2012,36(9):1367~1375
真蛸热休克蛋白90基因(HSP90)的克隆及表达
Molecular cloning and feature analysis of heat shock protein 90(HSP90)fromOctopus vulgaris
投稿时间:2012-01-07  修订日期:2012-02-18
DOI:DOI:10.3724/SP.J.1231.2012.27933
中文关键词: 真蛸  热休克蛋白90  克隆  实时荧光定量PCR
英文关键词: Octopus vulgaris  heat shock protein 90(HSP90)  molecular cloning  real-time quantitative PCR
基金项目:国家海洋公益性行业科研专项(201005013); 国家科技支撑计划(2011BAD13B08)
作者单位
孙田田 厦门大学海洋与地球学院 
苏永全 厦门大学海洋与地球学院 
洪婧妮 厦门大学海洋与地球学院 
邬阳 厦门大学海洋与地球学院 
张曼 厦门大学海洋与地球学院 
毛勇 厦门大学海洋与地球学院 
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中文摘要:
      为深入了解真蛸热应激响应机制,实验以真蛸的应激蛋白为研究对象,借助同源扩增和 cDNA末端快速扩增(rapid amplification of cDNA ends,RACE)技术获得真蛸热休克蛋白90(HSP90)的cDNA全序列(2 709 bp),它包含119 bp的5′非编码区(untranslated regions,UTR)、454 bp的3′UTR和2 136 bp的开放阅读框(opening reading frame,ORF),ORF共编码711个氨基酸,推算其分子量为81.5 ku,理论等电点为5.09。同源分析显示,所推导的氨基酸序列高度保守,并且含有HSP90家族特有的5个保守信号序列区域。实时荧光定量PCR分析表明,该基因在所有选取的组织中均有表达,肝组织中表达量最高。
英文摘要:
      Octopus vulgaris,which owns a number of unique stress protection mechanisms is considered to be the most intelligent species of all invertebrates.A 2 709 bp full length cDNA sequence of heat shock protein 90(HSP90) gene from O.vulgaris was obtained by homologous amplification and rapid amplification of cDNA ends(RACE).It consisted of a 119 bp 5′untranslated region(UTR),a 2 136 bp open reading frame(ORF)and a 454 bp 3′UTR.The inferred amino acids sequence was composed of 711 amino acids,whose molecular weight and isoelectric point were 81.5 ku and 5.09,respectively.Homologous analysis showed the inferred amino acids sequence was highly conserved and contained five classicalHSP90 signature sequences.Real time quantitative PCR(RT-PCR)results displayedHSP90 expressed in every tissues chosen,with the most in liver.This study which was the first time to obtainHSP90 gene from Cephalopod played an important role in studying stress mechanisms on the most intelligent invertebrate.
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