文章摘要
刘连为,许强华,陈新军.基于线粒体COⅠ和Cytb基因序列的北太平洋柔鱼种群遗传结构研究[J].水产学报,2012,36(11):1675~1684
基于线粒体COⅠ和Cytb基因序列的北太平洋柔鱼种群遗传结构研究
Studies on the population genetic structure of Ommastrephes bartramii in North Pacific Ocean based on the COⅠand Cytb gene sequence analysis
投稿时间:2012-01-18  修订日期:2012-02-24
DOI:10.3724/SP.J.1231
中文关键词: 柔鱼  COⅠ基因  Cytb基因  遗传多样性  遗传结构
英文关键词: Ommastrephes bartramii  COⅠgene  Cytb gene  genetic diversity  genetic structure
基金项目:国家“八六三”高技术研究发展计划(2012AA092303); 国家自然科学基金项目(NSFC41276156); 教育部博士点基金(20093104110002)
作者单位E-mail
刘连为 上海海洋大学海洋科学学院 M100301441@stmail.shou.edu.cn 
许强华 上海海洋大学海洋科学学院  
陈新军 上海海洋大学海洋科学学院  
摘要点击次数: 2006
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中文摘要:
      基于线粒体DNA的COⅠ和Cytb基因序列对北太平洋柔鱼2个产卵季节群体的遗传多样性和遗传结构进行分析。经PCR扩增与测序分别获得600bp COⅠ与481bp Cytb基因序列。二者多态性遗传参数统计显示,基于COⅠ和Cytb两基因序列的单倍型数、单倍型多样性指数(h)、核苷酸多样性指数(π)、平均核苷酸差异数(k)分别为24、0.729?0.033、0.00570?0.00325、3.421和28、0.852?0.016、0.00645?0.00373、3.101。分析认为,北太平洋柔鱼群体具有较高的单倍型多样性指数和较低的核苷酸多样性指数。单倍型邻接树、两两群体间的Fst值以及AMOVA分析结果均表明,北太平洋柔鱼2个产卵季节群体间的遗传差异不显著,不存在显著的群体遗传结构。初步认为,柔鱼2个产卵季节群体的产卵场与孵化期在时空分布上的连续性、柔鱼个体较强的游泳能力以及海流的作用,使得群体之间具有较强的基因流。
英文摘要:
      In this study, the genetic diversity and genetic structure of two spawning season cohorts of Ommastrephes bartramii in North Pacific Ocean were analyzed based on mtDNA COⅠand Cytb gene sequence analysis. 600bp of COⅠpartial sequences and 481bp of Cytb partial sequences were obtained by using PCR amplification and sequencing. The result revealed that the number of haplotype, haplotype diversities, nucleotide diversities and average nucleotide differences in COⅠand Cytb sequences were 24, 0.729?0.033, 0.00570?0.00325, 3.421 and 28, 0.852?0.016, 0.00645?0.00373, 3.101 respectively. All 7 populations were characterized by higher haplotype diversity and lower nucleotide diversity. A shallow topology of haplotype neighbor-joining (NJ) tree showed no phylogeographic structure among 7 populations. Analysis of molecular variance (AMOVA) and the fixation indices (Fst) of 7 populations showed that the genetic variance mainly came from individuals within populations, and no genetic differentiations between populations were detected from our study. It was supposed that extensive gene exchange between two spawning season cohorts was attributed to continuity of spawning ground and hatching date in temporal and spatial distribution, higher swimming speed of Ommastrephes bartramii individuals and influence of sea currents.
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