文章摘要
杨萍,柯虹乔,章超桦,洪鹏志.大眼金枪鱼头蛋白酶解物1 ku超滤组分的抗氧化活性及其理化性质[J].水产学报,2012,36(8):1297~1303
大眼金枪鱼头蛋白酶解物1 ku超滤组分的抗氧化活性及其理化性质
Antioxidant activity and physicochemical properties of 1 ku fraction by ultrafiltration of bigeye tuna (Thunnus obesus) head protein hydrolysate
投稿时间:2012-01-21  修订日期:2012-06-06
DOI:DOI:10.3724/SP.J.1231.2012.27959
中文关键词: 大眼金枪鱼  鱼头  酶解物  超滤  抗氧化活性
英文关键词: Thunnus obesus  fish head  enzymatic hydrolysate  ultrafiltration  antioxidant activity
基金项目:广东省自然科学基金项目(10152408801000031); 广东海洋大学自然科学基金(2010)
作者单位
杨萍 广东海洋大学食品科技学院 
柯虹乔 广东海洋大学食品科技学院 
章超桦 广东海洋大学食品科技学院 
洪鹏志 广东海洋大学食品科技学院 
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中文摘要:
      研究大眼金枪鱼头蛋白酶解物1 ku超滤组分体外的还原力、自由基清除能力及对衰老小鼠体内抗氧化能力酶活性的影响, 并分析1 kuKD超滤组分的一般营养成分、氨基酸组成及分子量分布, 为进一步分离纯化金枪鱼头抗氧化肽提供基础。体外实验结果显示, 1 kuKD超滤组分对羟基自由基、超氧阴离子和DPPH自由基的清除活性随浓度的增加而增强, IC50分别为1.38、0.73与0.93 mg/mL, 还原力也随浓度的增加而增大, 在浓度为12.5 mg/mL时为0.763; 体内实验结果显示, 灌胃30 mg/kg的1 kuKD超滤组分连续42 d, D-半乳糖致衰老小鼠肝组织的超氧化物歧化酶(SOD)活性、肝组织和血清的谷胱甘肽过氧化物酶(GSH-PX)活性力显著提高(P<0.05), )血清丙二醛(MDA)含量显著降低(P<0.01); 理化分析结果显示, 1 kuKD超滤组分(干基计)蛋白质含量为96.40%, 脂肪含量为0.11%, 灰分含量为4.86%, 疏水性氨基酸占氨基酸总量的35.8%, 活性组分分子量在1 8023800~2 5192746 u和422~922 u之间。
英文摘要:
      To produce bioactive peptides from the by-products of fish processing, bigeye tuna (Thunnus obesus) head was hydrolyzed by alcalase, and the tuna head protein hydrolysate (THPH) was fractionated through ultrafiltration membranes with a range of molecular weight cutoffs (MWCO) of 8, 5, 3 and 1 ku, respectively, to yield the fraction THPH-1 with MW distribution<1 ku. The antioxidant activities of THPH-1were evaluated using reducing power, free radical scavenging and aged model mice induced by D-galactose. The physicochemical properties of THPH-1 were analysed also. THPH-1 showed evident radical scavenging activity in a dose-dependent manner with the IC50 values for hydroxyl radical, superoxide radical and 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical being 1.38, 0.73 and 0.93 mg/mL respectively. The reducing power of THPH-1 was 0.763 at 12.5 mg /mL. The activities of superoxide dismutase (SOD) of liver and the activities of glutathione peroxidase (GSH-PX) of liver and serum in aged model mice were significantly increased (P<0.05) compared with that of the normal control group mice after 42 days administrated orally at a dose of 30 mg/kg THPH-1, and the content of malondialdehyde (MDA) of serum in aged model mice was significantly decreased (P<0.01).The physicochemical properties showed that THPH-1contained 96.40% of protein(on dry basis), 0.11% of fat and 4.86% of ash. The total hydrophobic amino acids content of THPH-1was 35.8% of the total amino acids content. The molecular weight distribution of the higher activity fractions of THPH-1were located at 1 802-2 519 u and 422-922 u.These results indicate that THPH-1from tuna head hydrolysate would be very fairly helpful in the preparation of antioxidative peptides and a beneficial ingredient for functional food.
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