文章摘要
俞菊华,李红霞,李建林,唐永凯,夏正龙,董在杰.建鲤FABP3基因分离及其多态性与增重的相关分析[J].水产学报,2012,36(12):1809~1818
建鲤FABP3基因分离及其多态性与增重的相关分析
Sequences analysis of jlFABP3s and the correlation between polymorphisms and body weight gain in Carpio cyprino var. jian
投稿时间:2012-02-09  修订日期:2012-06-15
DOI:DOI:10.3724/SP.J.1231.2012.27971
中文关键词: 建鲤  心肌型胞内脂肪酸结合蛋白  单核苷酸多态性  SNP-增重相关分析
英文关键词: Cyprinus carpio var. jian  heart and muscle fatty acid binding protein  single nucleotide polymorphism  SNP-weight gain correlated analysis
基金项目:水产分子育种共性技术的建立与应用(200903045); 国家“八六三”高技术研究发展计划(2011AA100401); 现代农业产业技术体系建设专项?(CARS-46)
作者单位E-mail
俞菊华 中国水产科学研究院淡水渔业研究中心 yujh@ffrc.cn 
李红霞 中国水产科学研究院淡水渔业研究中心  
李建林 中国水产科学研究院淡水渔业研究中心  
唐永凯 中国水产科学研究院淡水渔业研究中心  
夏正龙 中国水产科学研究院淡水渔业研究中心  
董在杰 中国水产科学研究院淡水渔业研究中心  
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中文摘要:
      胞内脂肪酸结合蛋白主要参与脂肪酸的胞外和胞内膜间转移,也参与调控细胞的生长和增殖。本实验分离了建鲤心肌型胞内脂肪酸结合蛋白jlFABP3a和3b基因,jlFABP3s有4个外显子和3个内含子组成,3a和3b阅读框相似性为93%,编码133个氨基酸,相似性为94%,内含子长度和序列存在着明显的差异。jlFABP3的系统进化和传统的分类地位一致。通过序列比对在3a和3b上分别找到26个和25个SNP位点。构建PCR-RFLP法检测了其中3个SNPs在建鲤群体中的分布,并与增重进行相关分析,结果C30G与检测群体雄鱼鱼种阶段、雌鱼鱼种和成鱼阶段增重显著相关(P<0.05),CC型个体增重显著快于CG型个体。G267T在5个家系中与雌鱼成鱼增重相关,在7个家系中不同基因型个体增重虽呈相同趋势但差异不显著(P>0.05)。同时考虑G267T和C30G位点,则发现GGCC个体在雌、雄鱼增重均最大,在雌、雄鱼中分别比GGCG个体快17%和12%。GGCC个体在实验样本中占了约9%,存在着较大的选育空间。
英文摘要:
      Fatty Acid Binding Proteins are mainly involved in fatty acid transfer in the extracellular and intracellular membrane, and also take part in the regulation of cell growth and proliferation. In this experiment, two heart and muscle fatty acid binding protein genes,jlFABP3a and 3b, were isolated from Cyprinus carpio var. jian. Consistent with other FABP3s, jlFABP3s contain four exons and three introns. The open reading frames of 3a and 3b encode 133 amino acids share 93% and 94% similarity with each other in DNA and protein level, respectively. And there was clear difference in sequences and lengths between two genes introns. Twenty-six and twenty-five SNPs were found on jlFABP3a and 3b respectively in Cryprinus carpio var. jian population using alignment sequences from different individuals. And genotypes in 3 SNP loci were detected using PCR-RFLP. The SNP-weight gain correlation analysis indicated C30G was significantly associated with male juvenile, female juvenile and adult fish weight gain (P<0.05) in total experimental samples. Individuals with CC genotype grew faster than CG individuals. G267T was significantly related with female adult fish weight gain (P<0.05) in 5 families, and three genotype individuals had same growth trends in the 7 populations, though no significant (P>0.05). Taking two SNPs (G267T and C30G) into account, correlation analysis revealed individuals with GGCC grew the fastest in female and male fish, and grew 17% and 12% faster than GGCG ones. In the experimental samples,the GGCC individuals occupy only about 9%, indicating the presence of large selection space, and can be used as reference in molecular assistant breeding of Cryprinus carpio var. jian.
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