文章摘要
赵良杰,何光喜,周小玉,贾佩峤,武震,宋固,张宏,刘其根.千岛湖大眼华鳊(Sinibrama macrops)形态度量学和D-loop序列分析[J].水产学报,2013,37(3):351~358
千岛湖大眼华鳊(Sinibrama macrops)形态度量学和D-loop序列分析
Morphology and D-loop sequence analysis of Sinibrama macrops from Qiandaohu Lake
投稿时间:2012-03-06  修订日期:2012-05-09
DOI:DOI:10.3724/SP.J.1231.2013.38015
中文关键词: 大眼华鳊(Sinibrama macrops),千岛湖,形态度量学,主成分分析,D-loop
英文关键词: Sinibrama macrops  Qiandaohu Lake  Morphology  Principle component analysis  D-loop
基金项目:国家自然科学基金
作者单位E-mail
赵良杰 上海海洋大学 农业部水产种质资源与利用重点开放实验室 a850924t@163.com 
何光喜 浙江杭州千岛湖发展有限公司  
周小玉 上海海洋大学 农业部水产种质资源与利用重点开放实验室  
贾佩峤 上海海洋大学 农业部水产种质资源与利用重点开放实验室  
武震 上海海洋大学 农业部水产种质资源与利用重点开放实验室  
宋固 上海海洋大学 农业部水产种质资源与利用重点开放实验室  
张宏 上海海洋大学 农业部水产种质资源与利用重点开放实验室  
刘其根 上海海洋大学 农业部水产种质资源与利用重点开放实验室 qgliu@shou.edu.cn 
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中文摘要:
      本文以千岛湖大眼华鳊(Sinibrama macrops)为材料通过形态度量学和分子生物学的方法对其进行了研究,旨在了解千岛湖中大眼华鳊(Sinibrama macrops)各地理种群是否发生分化。形态变量的主成分分析(PCA)结果的散布图显示:三个群体的标本重叠在一起,表明三个水域的大眼华鳊没有发生形态上的分化。通过对线粒体D-loop区的序列分析共发现了27个变异位点,35个单倍型。NJ树和单倍型网络图均未显示出各群体有分化的趋势。三个群体的平均碱基组成差异很小,且A T含量(64.82%)高于G C含量(35.18%),各群体均表现出高单倍型多样性(h)和低核苷酸多样性(π)的特点,汾口的遗传多样性最高(h=0.968?0.018,π=0.00476?0.00049),临岐次之(h=0.968?0.017,π=0.00453?0.00041),富文最低(h=0.937?0.037,π=0.00330?0.00035)。各群体间均具有较强的基因流(Nm>4),其中汾口和富文之间最强(Nm=27.40)。两两群体之间的遗传分化指数(Fst)都呈现出不显著的轻度分化(Fst<0.05,P>0.05)。主成分分析和线粒体序列分析综合表明千岛湖各水域的大眼华鳊属于一个随机交配群体。为了保护这一重要的渔业资源鱼类,建议继续保持千岛湖大眼华鳊的基因流格局,减少人为因素对该物种在库区活动的影响。
英文摘要:
      Morphological and genetic differences of three Sinibrama macrops populations from Qiandaohu Lake were compared by traditional morphological method and mitochondrial D-loop region sequence analysis. Based on the results of principal components analysis, specimens clustered together by scatter plots of scores could not be divided. It indicated that there is no morphological differentiation among all samples from Qiandaohu Lake. 27 polymorphic sites were detected yielding 72 haplotypes. NJ tree and network showed that the geographic distribution was ambiguous. There were similar base composition among the three populations, and the content of A T (64.82%) was higher than the content of G C (35.18%). High genetic variation (h) and low nucleotide diversity (π) were found in the three populations analyzed for D-loop, and the diversity of Fenkou (h=0.968?0.018, π=0.00476?0.00049) was the highest, Linqi (h=0.968?0.017, π=0.00453?0.00041) was the second, Fuwen (h=0.937?0.037, π=0.00330?0.00035) was the lowest. The values of genetic differentiation index (Fst) were lower than 0.05 and not significant (P>0.05). A strong gene flow among the populations were observed (Nm>4), and the Nm between Fenkou and Fuwen was highest (Nm= 27.40). The results of all supported the three geographical populations belong to a randomly mating population. Consequently, we suggest to keep the pattern of gene flow of S. macrops, and reduce the influence of human activities so as to protect this important species in fishery resources.
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