文章摘要
郑佳佳,彭丽莎,张小平,孙健栋,傅罗琴,胡彩虹,李卫芬.复合益生菌对草鱼养殖水体水质和菌群结构的影响[J].水产学报,2013,37(3):457~464
复合益生菌对草鱼养殖水体水质和菌群结构的影响
Effects of complex probiotics on water quality and microbial communities in a grass carp (Ctenopharyngodon idellus) culture.
投稿时间:2012-04-12  修订日期:2012-12-05
DOI:DOI:10.3724/SP.J.1231.2013.38105
中文关键词: 复合益生菌  草鱼  水质  菌群结构
英文关键词: complex probiotics  grass carp  water quality  microbial community structure
基金项目:国家重点基础研究发展规划(973)项目(2009CB118705);公益性行业(农业)科研专项(201203083)
作者单位E-mail
郑佳佳 浙江大学动物科学学院 06dwkxzjj@zju.edu.cn 
彭丽莎 浙江大学动物科学学院  
张小平 浙江大学动物科学学院  
孙健栋 浙江大学动物科学学院  
傅罗琴 浙江大学动物科学学院  
胡彩虹 浙江大学动物科学学院  
李卫芬 浙江大学动物科学学院 wfli@zju.edu.cn 
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中文摘要:
      研究了草鱼养殖水体中添加复合益生菌水质调节剂对水体水质和菌群的调节作用。结果表明:处理组的氨氮、亚硝酸盐氮和总氮浓度一直低于对照组,但差异不显著;处理组硝酸盐氮浓度低于对照组,且在第18天时下降了56.59%(P<0.05);处理组的总无机氮含量低于对照组,且在第15天时下降了28.75%(P<0.05)。处理组正磷酸盐和总磷浓度略低于对照组,无显著差异。第15天水样的454焦磷酸盐测序结果表明,与对照组相比,处理组菌群多样性更高,厚壁菌门和变形菌门分别减少了91.21%(P<0.01)和21.75%(P<0.01),拟杆菌、放线菌和蓝细菌分别增加了288%(P<0.01)、435%(P<0.01)和848%(P<0.01)。在变形菌门中,α-变形杆菌和β-变形杆菌分别比对照组提高了318%(P<0.01)和18%,γ-变形杆菌比对照组降低了78.82%(P<0.01)。
英文摘要:
      This experiment was conducted to study the effects of complex probiotics on water quality and microbial community structure in grass carp culture. The results of water quality determination showed that compared with the control group, the concentration of ammonia nitrogen, nitrite nitrogen and total nitrogen in treatment group was lower in the whole experiment, respectively; At 18th d, the concentration of nitrate nitrogen in treatment group was decreased by 54.49% (P<0.05). At 15th d, the concentration of total inorganic nitrogen in treatment group was decreased by 28.75%(P<0.05). Orthophosphate and total phosphorus was no difference between the two groups. The 454-Pyrosequencing results showed that the composition of bacterial communities was highly variable between control and treatment group, and microbial diversity of treatment was higher than that of contorl. Firmicutes and Proteobacteria was decreased by 91.21% (P<0.01) and 21.75% (P<0.01) respectively. Bacteroidetes, Actinobacteria and Cyanobacteria was increased by 2.88 times (P<0.01), 4.35 times (P<0.01) and 8.48 times (P<0.01) respectively. Among Proteobacteria,Alphaproteobacteria and Betaproteobacteria was elevated by 3.18 times (P<0.01) and 18% respectively, when Gammaproteobacteria was reduced by 78.82% (P<0.01).
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