文章摘要
贾雯,黄翔鹄.侧孢短芽孢杆菌(Brevibacillus laterosporus)溶藻活性代谢产物对虾池颤藻(Oscillatoria sp.)溶藻效果研究[J].水产学报,2013,37(3):465~472
侧孢短芽孢杆菌(Brevibacillus laterosporus)溶藻活性代谢产物对虾池颤藻(Oscillatoria sp.)溶藻效果研究
Research on algicidal effect of bioactive metabolites of Brevibacillus laterosporus to Oscillatoria sp. in shrimp pond
投稿时间:2012-07-22  修订日期:2012-12-06
DOI:DOI:10.3724/SP.J.1231.2013.38269
中文关键词: 溶藻细菌  除菌体滤液  颤藻  叶绿素a  藻蓝蛋白
英文关键词: algae-lysing bacteria  sterilization filtrate  Oscillatoria sp.  Chlorophyll-a  phycocyanin protein
基金项目:科技部农业科技成果转化资金(批准号:2010GB2E000351);广东省自然基金(批准号:9152408801000003)
作者单位E-mail
贾雯 广东海洋大学水产学院 amethyst_wen@126.com 
黄翔鹄 广东海洋大学水产学院 hxh166@126.com 
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中文摘要:
      颤藻(Oscillatoria sp.)为对虾养殖池塘中的一种常见的有害蓝藻。本实验以侧孢短芽孢杆菌(Brevibacillus laterosporus)的除菌体滤液和颤藻为基础,研究各自在不同阶段的溶藻效果,通过测定藻体干重、叶绿素a含量以及藻蓝蛋白含量,探究溶藻效果的最佳作用阶段及其作用机理,为改善对虾养殖水环境提供一定的理论基础。实验结果表明:稳定期和衰亡期的除菌体滤液对颤藻的溶藻效果均影响极显著(P<0.01):7天后颤藻干重分别减少了51.77%、47.04%,叶绿素a含量分别降低了67.60%、59.13%,藻蓝蛋白含量分别增加了33.51%、30.97%;溶藻细菌除菌体滤液对延滞期的颤藻溶藻效果影响极显著(P<0.01):7天后颤藻干重减少了63.90%,叶绿素a含量下降了69.72%,藻蓝蛋白含量升高了54.17%。因此,本研究显示侧孢短芽孢杆菌培养至稳定期的除菌体滤液对延滞期颤藻的溶藻效果最好。
英文摘要:
      Oscillatoria sp. is a so common and harmful cyanophytes in shrimp culture pond. In this experiment, sterilization filtrate of Brevibacillus laterosporus and Oscillatoria sp.were used to study algicidal effects in their different growth stages. Algae dry weight, chlorophyll-a and phycocyanin protein contents were measured in order to explore the best algae-lysing period and algicidal mechanism. The final aim is that providing theoretical basic for improving water envinment in shrimp pond. The results show that algicidal effects of sterilization filtrate to Oscillatoria sp. are very significant(P<0.01)when the algae-lysing bacteria in the stable and decline phases. After 7 days, algae dry weight were reduced by 51.77%、47.04% separately, chlorophyll-a content were decreased by 67.60% , 59.13% respectively, phycocyanin protein content were severally increased by 33.51%、30.97%. And similarly, the algicidal effect of sterilization filtrate of alage-lysing bacteria is so conspicuous(P<0.01)to the lag phase Oscillatoria sp.. 7 days later, algae dry weight were reduced by 63.90%, chlorophyll-a content were decreased by 69.72%, phycocyanin protein content were increased by 54.17%. The conclusion is that sterilization filtrate of Brevibacillus laterosporus in the stable phase has the best algicidal effect to the lag phase Oscillatoria sp..
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