文章摘要
迟淑艳,谭北平,杨奇慧,董晓慧,刘泓宇.投饲频率对摄食含有晶体蛋氨酸饲料的凡纳滨对虾生长性能的影响[J].水产学报,2013,37(5):761~767
投饲频率对摄食含有晶体蛋氨酸饲料的凡纳滨对虾生长性能的影响
Effects of feeding frequency on growth performance of Litopenaeus vannamei fed diet with crystalline methionine
投稿时间:2012-08-27  修订日期:2013-01-28
DOI:DOI:10.3724/SP.J.1231.2013.38309
中文关键词: 凡纳滨对虾  投饲频率  晶体蛋氨酸  生长性能
英文关键词: Litopenaeus vannamei  feeding frequency  crystalline methionine  growth performance
基金项目:公益性行业(农业)科研专项(201003020);广东省高等学校珠江学者特聘教授基金(GDUPS-2011);湛江市科技攻关计划(2010C3113013)
作者单位
迟淑艳 广东海洋大学水产学院 
谭北平 广东海洋大学水产学院 
杨奇慧 广东海洋大学水产学院 
董晓慧 广东海洋大学水产学院 
刘泓宇 广东海洋大学水产学院 
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中文摘要:
      为研究改变投饲频率后晶体蛋氨酸吸收利用的效率,实验以凡纳滨对虾为研究对象,以高鱼粉组为正对照组,以低鱼粉且不添加蛋氨酸组为负对照组,添加晶体蛋氨酸(MET)组为实验组,配制3种等氮等脂的饲料。MET组日投饲频率分别为2(7:00和21:00)、4(7:00,12:00,18:00 和 21:00)和6次(7:00,9:30,12:00,15:00,18:00 和21:00),正对照组和负对照组日投喂4次。将初重为(1.27?0.01) g的对虾随机分为3组,每组3个重复,每重复30尾虾,养殖5周,水温29~30 ℃。结果显示,日投喂6次的MET组增重率和正对照组相比差异不显著,全虾粗蛋白含量显著高于日投喂4次的,MET组总蛋白酶活性随投喂次数增加而增加,日投喂4次和6次的处理组与正对照组差异不显著。结果表明,投饲频率由4次增加至6次,晶体蛋氨酸组凡纳滨对虾的增重率、体蛋白的沉积和总蛋白酶的活性得到改善,增加投喂频率有利于提高游离氨基酸的利用效率。
英文摘要:
      This study focused on evaluating the effects of feeding frequency on growth performance of Litopenaeus vannamei fed diet with crystalline methionine.The growth experiment was conducted to test three dietary treatments including fish meal(32%)as positive control,fish meal(16%)with methionine deficiency as negative control,and fish meal(16%)with crystalline L-methionine(MET)as the test diet.Feeding frequency trial was conducted in order to explore the difference in absorption of crystalline methionine and amino acid from intact protein(positive control and negative control).Shrimp were fed MET dietary in feeding frequency 2(7:00 and 21:00),4(7:00,12:00,18:00 and 21:00),and 6 times-day(7:00,9:30,12:00,15:00,18:00 and 21:00),the positive control and negative control were fed 4 times per day only.Each treatment was randomly assigned to triplicate groups of 30 shrimps(initial weight 1.27?0.01 g)per aquarium.Shrimp were maintained in aquaria for 5 weeks at water temperature that ranged from 29 to 30 ℃.The results showed that shrimp fed MET diet 6 times per day had no difference in weight gain with positive control(P>0.05).Crude protein contents of shrimp body of shrimp fed MET 6 times per day were significantly higher than that 4 times per day(P<0.05).Total protease activities of shrimp increased with feeding frequency increased,which fed MET 4 times per day and 6 times per day had no difference with positive control(P>0.05).These results indicate that the shrimp fed low fishmeal diet supplemented with methionine had better weight gain,body protein deposition and total protease activities with feeding frequency from 4 to 6 times per day.Increased feeding frequency could improve utilization of free amino acid.
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