文章摘要
肖同乾,鲁翠云,张明昭,顾颖,孙效文.八种鲤养殖品种线粒体Cyt b基因的遗传多样性和系统进化分析[J].水产学报,2013,37(3):344~350
八种鲤养殖品种线粒体Cyt b基因的遗传多样性和系统进化分析
Genetic diversity and phylogenetic analysis of eight Cyprinoid aquaculture breeds based on mitochondrial Cyt b gene
投稿时间:2012-09-28  修订日期:2012-12-14
DOI:DOI:10.3724/SP.J.1231.2013.38358
中文关键词: 鲤养殖品种  遗传多样性  细胞色素b
英文关键词: cyprinoid aquaculture breeds  genetic diversity  Cytochrome b
基金项目:公益性行业(农业)科研专项(200903045);863计划项目(2011AA100402-5);国家科技基础条件平台建设运行项目。
作者单位E-mail
肖同乾 中国水产科学院研究院黑龙江水产研究所 xiaotongqian@126.com 
鲁翠云 中国水产科学院研究院黑龙江水产研究所  
张明昭 中国水产科学院研究院黑龙江水产研究所  
顾颖 中国水产科学院研究院黑龙江水产研究所  
孙效文 中国水产科学院研究院黑龙江水产研究所 sunxw2002@163.com 
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中文摘要:
      对八种鲤养殖品种的线粒体Cyt b基因部分序列进行测定,并比较分析其遗传多样性和系统进化关系,结果获得425bp的Cyt b序列,其中T、C、A、G的平均含量分别为26.8﹪、29﹪、29.7﹪、14.5﹪,A T含量(56.5﹪)高于G C含量(43.5﹪),翻译为141个氨基酸,其中突变多数存在于缬氨酸和异亮氨酸之间(两个氨基酸都为不带电荷的疏水性氨基酸),其变异不影响Cyt b的功能,说明该蛋白是一个进化缓慢的蛋白,适合用于遗传进化分析。140个个体中共检测到4个单倍型,其中75.5﹪的个体属于单倍型Hap 2,而单倍型Hap 1主要分布在三种表现型为镜鲤的品种。AMOVA分析结果和利用Kimurar2模型分析的系统发育关系结果都表明德国镜鲤、散磷镜鲤和红镜鲤亲缘关系较近,而蓝鳞鲤、荷包红鲤抗寒品系和高寒鲤亲缘关系较近,分析结果与其选育背景相一致。同时发现,松浦鲤的遗传多样度较赵莹莹所测有所降低,建议之后的保种过程中加强管理以保证其遗传多样性。
英文摘要:
      The mitochondrial cytochrome b gene fragments of eight cyprinoid aquaculture breeds were sequenced and their genetic diversity and cluster analysis were compared. Mean content of T, C, A, G, are 26.8﹪, 29﹪, 29.7﹪, 14.5﹪, respectively, and A plus T is higher than G plus C. The mutations in the 141 amino acids translated by 425bp of Cyt b gene fragment, could not affect its function dueing that the majority were generated between the valin and isoleucine which were unchanged and hydrophobic. The result demonstrated that the evolution of this protein was slowly, and it was adapted for the genetic analysis. Five haplotypes were detected from 140 individuals, of which 75.5 percents belong to the Hap2, and the Hap1 was mainly distributed in the mirror carp. The AMOVA and cluster analysis based on Kimura 2-parameter model exhibited very close relationship among Germany mirror carp, Scattered mirror carp, Red mirror carp, and also revealed that the relationship among Cyprinus carpio blue var, Cold-resistance strain of red purse carp, Frigid carp was close. The results were in accordance with their breeding backgrounds.
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