文章摘要
隗健凯,柳承璋,张晓军,王兵,董波,李富花,相建海.基于中国明对虾不同组织EST数据的基因表达差异分析[J].水产学报,2013,37(5):661~671
基于中国明对虾不同组织EST数据的基因表达差异分析
Differential gene expression analysis based on expressed sequence tags(EST) from different tissues of Fenneropenaeus chinensis
投稿时间:2012-10-09  修订日期:2013-01-28
DOI:DOI:10.3724/SP.J.1231.2013.38371
中文关键词: 中国明对虾  表达序列标签(EST)  组织差异  组织高表达基因
英文关键词: Fenneropenaeus chinensis  expressed sequence tags(EST)  tissue variability  tissue highly expressed genes
基金项目:国家“八六三”高技术研究发展计划(2012AA10A404,2012AA092205);国家自然科学基金项目(30972245,41076101,31172396)
作者单位
隗健凯 中国科学院海洋研究所实验海洋生物学重点实验室 
柳承璋 中国科学院海洋研究所实验海洋生物学重点实验室 
张晓军 中国科学院海洋研究所实验海洋生物学重点实验室 
王兵 中国科学院海洋研究所实验海洋生物学重点实验室 
董波 中国科学院海洋研究所实验海洋生物学重点实验室 
李富花 中国科学院海洋研究所实验海洋生物学重点实验室 
相建海 中国科学院海洋研究所实验海洋生物学重点实验室 
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中文摘要:
      对前期获得的中国明对虾头胸部、血液、眼柄、卵巢4种组织的EST测序数据进行了生物信息学挖掘和分析。4种组织的原始EST序列分别为10 446条、2 690条、1 067条和1 282条,通过聚类拼接得到unigene 3 454条、1 053条、406条和544条,对其进行了NR、GO、KEGG等数据库的功能注释,并在此基础上进行了组织差异分析。通过同源比对的方法找出了各组织特异的转录本,并根据注释信息将其进行了GO功能分类。结果显示,血液组在细胞杀伤、迁移和节律等功能分类上特异性富集;眼柄组在色素生成、信号传递和生物学调控等功能分类上特异性富集;卵巢组在营养贮存运输、繁殖和定位等功能分类上特异性富集。拼接时聚类到一起的EST数目在一定程度上可以代表基因的表达量,据此对各组织的高表达基因进行了分析。结果显示,peritrophinelongation factor 1-alphathrombospondinarginine kinase 4个基因在组织中分布较为广泛且表达量高,提示其可能参与对虾多种重要的生物学过程。而在血液组中注释到的高表达基因还包括penaeidincytochrome c oxidase和14-3-3 like protein基因,在眼柄组中注释到的高表达基因还包括arrestinrhodopsin基因。此外,为了解各组织共有基因的差异表达情况,对peritrophinperoxiredoxin进行了分析,发现了peritrophin基因的多形式和高表达现象。
英文摘要:
      Bioinformatics analysis was conducted based on the acquired expressed sequence tags(ESTs)data from different tissues of Fenneropenaeus chinensis.A total of 10 446,2 690,1 067 and 1 282 original ESTs were obtained from cephalothorax,blood,eyestalk,and ovary of adult F.chinensis respectively.By clustering and assembling,3 454,1 053,406 and 544 unigenes were generated,and then annotated by searching in NR,GO,KEGG databases.The tissue specific transcripts were identified through sequence homology analysis and classified by GO annotation.The result indicated that specific transcripts in blood group were specifically enriched in the GO term of virion part,locomotion,rhythmic process,cell killing and multi organism process.Specific transcripts in eyestalk group were specifically enriched in the GO term of molecular transducer activity,response to stimulus,multicellular organismal process,pigmentation and biological regulation.Specific transcripts in ovary group were specifically enriched in the GO term of nutrient reservoir activity,reproduction,anatomical structure formation,transporter activity and establishment of localization.We also analyzed the highly expressed genes in each tissue according to the number of ESTs of each unigene.The result indicated that genes encoding peritrophin,elongation factor 1-alpha,thrombospondin and arginine kinase were widespread and highly expressed in tissues,suggesting that they were involved in a variety of important biological processes in shrimp.In addition,genes encoding penaeidin,cytochrome c oxidase and 14-3-3-like protein were highly expressed in blood group and genes encoding arrestin and rhodopsin were highly expressed in eyestalk group.For understanding the expressions of common genes,we analyzed genes related to peritrophin and peroxiredoxin,revealing that genes of peritrophin in F.chinensis were multiform and highly expressed.This result may provide reference for further functional study of peritrophin.
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