文章摘要
郭 香,李 琪,孔令锋,于 红.基于微卫星标记整合长牡蛎遗传图谱[J].水产学报,2013,37(6):823~829
基于微卫星标记整合长牡蛎遗传图谱
Microsatellite-based consensus map of the Pacific oyster (Crassostrea gigas)
投稿时间:2012-11-09  修订日期:2013-03-15
DOI:10.3724/SP.J.1231.2013.38423
中文关键词: 长牡蛎,微卫星,整合图谱,染色体重排,共显性标记
英文关键词: Crassostrea gigas, microsatellite, consensus map,chromosomal rearrangements, co-dominat markers
基金项目:国家高技术研究发展计划;国家重点基础研究发展计划
作者单位E-mail
郭 香 中国海洋大学教育部海水养殖重点实验室 guoxiang432@yahoo.com.cn 
李 琪 中国海洋大学教育部海水养殖重点实验室 qili66@ouc.edu.cn 
孔令锋 中国海洋大学教育部海水养殖重点实验室  
于 红 中国海洋大学教育部海水养殖重点实验室  
摘要点击次数: 2020
全文下载次数: 1484
中文摘要:
      连锁图谱是分子标记辅助育种和图位克隆的前提条件。但是,已发表的长牡蛎(Crassostrea gigas)遗传图谱上的共显性标记数量仍然较低。微卫星标记是可重复性良好,多态性丰富的共显性标记,可以在不同的家系和实验室间通用,是构建整合图谱理想的锚定标记之一。为了提高长牡蛎遗传图谱上的微卫星标记密度,本研究利用6个家系图谱间的共有微卫星标记作为锚定标记,构建了长牡蛎的整合图谱。该整合图谱包含161个微卫星标记,覆盖10个连锁群,图谱长度和平均间距分别为615.4 cM和3. 8 cM,各连锁群的标记数介于10~24之间,连锁群长度为47.3~73.3 cM,是目前密度最高的长牡蛎微卫星图谱。不同作图家系连锁群上的标记分组保持一致,但标记顺序出现差异。该图谱将为今后长牡蛎的遗传育种研究提供新的遗传工具。
英文摘要:
      Linkage map is basic prerequisites for marker-assisted selection and map-based cloning. However, the number of co-dominant genetic markers on linkage maps published for the Pacific oyster (Crassostrea gigas) is still low. Microsatellite markers are inherited as co-dominant, which are reproducible and high polymorphism. They are easily transferred among populations and laboratories, and one of ideal joining markers to construct a consensus map. In order to improve the number of microsatellite markers, a consensus map of C. gigas was constructed based on common microsatellite markers among six mapping populations. The consensus map contained 161 microsatellite markers, spanning 10 linkage groups. The map length and average genetic distance is respectively 615.4 cM and 3.8 cM. The number of loci on linkage group ranged from 10 to 24 and the genetic length of linkage group varied from 47.3 to 73.3 cM. At present, this consensus map is the densest SSR-based map of C. gigas. Among different mapping populations, the grouping of markers is consistent; however, the order of markers is a little different, which likely attributed to polymorphism for chromosomal rearrangements in the natural population of Pacific oysters. The consensus map will provide a new reference tool for genetics and breeding of C. gigas in the future.
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