文章摘要
黄贻涛,王艺磊.杂色鲍同种移植炎症因子1的克隆及其在应激下的表达[J].水产学报,2013,37(6):830~839
杂色鲍同种移植炎症因子1的克隆及其在应激下的表达
Molecular cloning and expression of Allograft Inflammatory Factor in Haliotis diversicolor under stresses
投稿时间:2012-12-03  修订日期:2013-03-31
DOI:10.3724/SP.J.1231.2013.38463
中文关键词: 杂色鲍  同种移植炎症因子  高温  缺氧  副溶血弧菌
英文关键词: Haliotis diversicolor, Allograft Inflammatory Factor(AIF), thermal, hypoxia, Vibrio parahaemolyticus
基金项目:国家自然科学基金
作者单位E-mail
黄贻涛 集美大学水产学院 396978240@qq.com 
王艺磊 集美大学水产学院 ylwang@jmu.edu.cn 
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中文摘要:
      同种移植炎症因子 AIF-1(Allograft Inflammatory Factor 1, AIF-1)是一种由干扰素γ诱导的含有EF-hand结构域的钙离子结合蛋白,其功能主要是参与移植排斥、免疫炎症反应、自身炎性和非炎性的损伤等。本文首次克隆了杂色鲍(Haliotis diversicolor)AIF-1基因cDNA全序列,其全长为942 bp,开放阅读框为456 bp,编码151个氨基酸。实时荧光定量PCR结果表明:AIF-1在杂色鲍各组织中均有表达,其中在血淋巴和鳃中表达量最高。高温应激下,AIF-1在鳃组织中各时相表达均显著上调,并在温度升至31℃时达到最高。而血淋巴和肝胰腺中AIF-1在高温应激前4个时相表达无显著差异,到了96 h均显著上调。缺氧应激下,AIF-1在血淋巴中表达变化没有显著差异,而鳃中24 h显著下调,192 h显著上调。副溶血弧菌感染实验表明AIF-1基因在感染后3 h、24 h和48 h均检测到AIF-1的表达量显著上调。高温和缺氧应激以及弧菌感染均显示AIF-1基因表达量发生显著变化,说明AIF-1基因可能作为免疫因子在应激等状况下发挥重要作用。
英文摘要:
      Allograft Inflammatory Factor-1(AIF-1)is a kind of calcium-binding protein with EF-hand domain. AIF-1 mRNA is robustly induced by IFN-γ in murine macrophages. It involves in transplant rejection, immune inflammatory reaction, non-inflammatory injury and so on. In this study, a molluscan AIF-1 gene, HdAIF-1, was cloned for the first time from Haliotis diversicolor. Its full-length cDNA sequence is 942 bp, with a 456 bp open reading frame encoding a protein of 151 aa. Quantitative real-time PCR results indicated that HdAIF-1 could be detected in all examined tissues, with the highest level in hemolymph and gill. Under thermal stress, HdAIF-1 was up-regulated in gill significantly at temperature-rise period and achieved the highest level when up to 31℃. However, the expression level of HdAIF-1 in hemolymph and hepatopancreas did not show significant difference between thermal group and control group from the first phase to the fourth, and its expression level was up-regulated significantly at 96 h in these two tissues. Under hypoxia stress, the expression level of HdAIF-1 in hemolymph was no significant difference between control and exposed groups. However, it was down-regulated at 24 h and up-regulated in 192 h remarkably in gill. After Vibrio parahaemolyticus challenge, the HdAIF-1 expression level achieved the highest level at the 3 h in hemolymph, reduced to the control level at 12 h, and then up-regulated significantly at both 24 h and 48 h. These results suggest that HdAIF-1 may play important roles as an immune factor under different stresses.
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