文章摘要
齐晓艳,董迎辉,姚韩韩,周小龙,林志华.文蛤30个微卫星标记的开发及在斧文蛤和帘文蛤[J].水产学报,2013,37(8):1147~1154
文蛤30个微卫星标记的开发及在斧文蛤和帘文蛤
Identification of 30 Microsatellite Markers in Meretrix meretrix and their transferability in Meretrix lamarckii and Meretrix lyrata
投稿时间:2012-12-11  修订日期:2013-04-27
DOI:DOI:10.3724/SP.J.1231.2013.38484
中文关键词: 文蛤,G-SSRs,EST-SSRs,多态性,通用性
英文关键词: Mereteix meretrix  G-SSRs  EST-SSRs  polymorphism
基金项目:国家自然科学基金项目(30972255)、国家现代贝类产业技术体系项目(CARS-48)、浙江省自然科学基金(Y3110080)、宁波市科技创新团队项目(2011B82017)。
作者单位E-mail
齐晓艳 宁波大学 海洋学院 宁波 wellnbu@126.com 
董迎辉 浙江万里学院 浙江省水产种质资源高效利用技术研究重点实验室 宁波  
姚韩韩 浙江万里学院 浙江省水产种质资源高效利用技术研究重点实验室 宁波  
周小龙 浙江万里学院 浙江省水产种质资源高效利用技术研究重点实验室 宁波  
林志华 浙江万里学院 浙江省水产种质资源高效利用技术研究重点实验室 宁波 zhihua9988@126.com 
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中文摘要:
      利用磁珠富集法构建的基因组文库和EST文库开发了30个文蛤多态性微卫星标记。8个G-SSR和22个EST-SSR多态性位点共获得140个等位基因,平均等位基因数为4.67,多态性信息含量、观测杂合度、期望杂合度分别介于0.172~0.744、0.040~0.720、0.187~0.793之间;G-SSRs的平均等位基因数、平均多态性信息含量、平均观测杂合度和平均期望杂合度都较EST-SSRs高;Hardy-Weinberg平衡检测发现,24个位点偏离了平衡状态;利用BLASTx对含多态性SSR位点的EST进行功能注释,11个位点来自注释基因序列。 将30对文蛤多态性SSR引物分别在斧文蛤和帘文蛤中进行了通用性检测,通用率分别为30%和40%。这些微卫星多态性位点的获得,为进一步开展文蛤遗传多样性分析、种质资源评价、分子标记辅助选育等奠定基础,并且可用于文蛤、斧文蛤和帘文蛤的比较作图、基因发掘和QTL定位等研究。
英文摘要:
      30 microsatellite loci of Mereteix meretrix were developed using microsatellite -enhanced genomic library and expressed sequence tags ( ESTs ) . Genetic diversity was analyzed in a population consisting of 30 Individuals . 8 G-SSRs and 22 EST-SSRs were found to be polymorphic. In these 30 microsatellite loci , a total of 140 alleles were identified , the number of alleles varied from 3 to 6 , with a mean of 4.67 alleles per locus. Polymorphic information content ( PIC ) ranged from 0.172 to 0.744 . The observed heterozygosity ( Ho ) was from 0.040 to 0.720 , while the expected heterozygosity ( He ) was from 0.187 to 0.793 . The mean values of number of alleles , PIC , Ho , and He of G-SSRs were higher than those of the EST-SSRs . 24 of the 30 loci deviated from the Hardy-Weinberg equilibrium after Bonferroni correction . 11 EST-SSR identified from annotated genes were expected to be useful for mapping these genes in linkage maps . Interspecific transferability of the 30 markers revealed that 12 and 9 were polymorphic in M. lamarckii and M. lyrata , respectively. These loci obtained will lay a foundation for the study of genetic diversity , germplasm resources evaluation , marker-assisted selection ( MAS ) and other relevant research in M. meretrix , and these novel polymorphic SSR markers can be used for comparative mapping, gene tagging and QTL mapping among M. meretrix, M. lamarckii and M. Lyrata.
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