文章摘要
刘连为,许强华,陈新军,闫杰,余为,王从军.基于线粒体DNA分子标记的东太平洋茎柔鱼群体遗传多样性比较分析[J].水产学报,2013,37(11):1618~1625
基于线粒体DNA分子标记的东太平洋茎柔鱼群体遗传多样性比较分析
Comparative analysis of genetic diversity of Dosidicus gigas from eastern Pacific Ocean based on molecular markers of mitochondrial DNA
投稿时间:2012-12-15  修订日期:2013-03-09
DOI:DOI:10.3724/SP.J.1231.2013.38490
中文关键词: 茎柔鱼  遗传多样性  遗传结构  细胞色素b基因  细胞色素氧化酶Ⅰ基因
英文关键词: Dosidicus gigas  genetic diversity  genetic structure  Cytb gene  COⅠ gene
基金项目:国家自然科学基金项目(41276156);上海市科委重大项目(12231203900);国家发改委产业化专项(2159999)
作者单位
刘连为 上海海洋大学海洋科学学院 
许强华 上海海洋大学海洋科学学院 
陈新军 上海海洋大学海洋科学学院 
闫杰 上海海洋大学海洋科学学院 
余为 上海海洋大学海洋科学学院 
王从军 上海海洋大学海洋科学学院 
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中文摘要:
      为合理开发利用东太平洋茎柔鱼资源,需要准确掌握其种群结构。基于线粒体细胞色素b(Cytb)与细胞色素氧化酶Ⅰ(COⅠ)2个分子标记对茎柔鱼赤道海域与秘鲁外海2个地理群体的遗传多样性现状进行比较分析。分析认为,基于Cytb基因所有序列得到2个群体总的单倍型数、单倍型多样性指数、核苷酸多样性指数及平均核苷酸差异数分别为7、(0.397±0.079)、(0.001 09±0.000 96)和0.600,秘鲁外海群体单倍型多样性指数仅为(0.282±0.101),明显低于赤道海域群体。基于COⅠ基因所有序列得到2个群体总的单倍型数、单倍型多样性指数、核苷酸多样性指数及平均核苷酸差异数分别为17、(0.787±0.051)、(0.002 90±0.001 38)和1.802。单倍型网络关系图及两两群体间遗传分化系数Fst分析结果显示,赤道海域群体与秘鲁外海群体不存在显著的遗传差异(Cytb:Fst=0.005 91,P>0.05;COⅠ:Fst=0.055 23,P>0.05),2个地理群体可能在赤道海域海流的作用下发生基因交流。单倍型网络关系图、中性检验和核苷酸不配对分析结果均表明,茎柔鱼经历过近期群体扩张事件,发生群体扩张的时间在138 900~167 900年前。
英文摘要:
      The worldwide climate and marine environmental changes have significant impacts on the distributions and fishery resources abundance fluctuations of Dosidicus gigas in the East Pacific Ocean.It is important to investigate genetic variance and population genetic structure of D.gigas in order to exploit and manage this squid scientifically.In this study,the genetic diversity and the genetic differentiation between two geographical populations of D.gigas were analyzed based on two mitochondrial DNA molecular markers.The results showed that the number of haplotype,haplotype diversities,nucleotide diversities and average nucleotide differences were 7,(0.397±0.079),(0.001 09±0.000 96) and 0.600 respectively in all Cytb sequences of two geographical populations,and haplotype diversity in population off the coast of Peru was only(0.282±0.101)which was significantly lower than that of equatorial waters.On the other hand,the number of haplotype,haplotype diversities,nucleotide diversities and average nucleotide differences in all COⅠ sequences were 17,(0.787±0.051),(0.002 90±0.001 38)and 1.802 respectively.The pairwise fixation index Fst and median network revealed lack of genetic structure between populations of equatorial waters and off the coast of Peru(Cytb:Fst=0.055 23,P>0.05;COⅠ:Fst=0.005 91,P>0.05),and this might be caused by equational oceanographic current.The minimum spanning network tree,mismatch distribution analysis and neutrality tests suggested that D.gigas experienced a recent population expansion,possibly 138 900 to 167 900 years ago.
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