文章摘要
刘襄河,叶超霞,郑丽勉,欧成成,王安利.饲料糊精水平对暗纹东方鲀幼鱼生长、消化酶活性和血液生化指标的影响[J].水产学报,2013,37(9):1359~1368
饲料糊精水平对暗纹东方鲀幼鱼生长、消化酶活性和血液生化指标的影响
Effect of dietary dextrin levels on growth,activities of digestive enzyme and blood biochemical indices of juvenile obscure puffer(Takifugu obscurus)
投稿时间:2013-02-24  修订日期:2013-05-10
DOI:DOI:10.3724/SP.J.1231.2013.38589
中文关键词: 暗纹东方鲀  生长  消化酶  血浆生化指标
英文关键词: Takifugu obscurus  growth  digestive enzyme  plasma biochemistry indices
基金项目:广东省重点实验室建设项目(2009A060800019);省部产学研结合项目(2011B090500009);广东省高等学校科技创新重点项目(CXZD1114)
作者单位
刘襄河 华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室 
叶超霞 华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室 
郑丽勉 华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室 
欧成成 华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室 
王安利 华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室 
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中文摘要:
      配制等蛋白质(42%),等脂肪(9%),糊精水平分别为10%、15%、20%、25%、30%的5组饲料,饲养暗纹东方鲀幼鱼[初始体质量为(10.25±0.51)g]60 d,考察饲料中不同糊精水平对其生长、消化酶活性和血液生化指标的影响。结果发现,随着饲料糊精水平的升高,暗纹东方鲀幼鱼的特定生长率和饲料效率先增加后降低,25%组最高,并显著高于10%和15%组。30%组鱼的脏体比和肝体比显著高于10%组,同时其肝脂肪含量也显著高于其他组。10%组的肝糖原和血浆总氨基酸含量显著低于其他组。10%和15%组鱼的肝胰脏和肠道中淀粉酶活性显著低于其他实验组,而其肝胰脏中胰蛋白酶活性显著高于30%组。25%和30%组的血浆总胆固醇和甘油三酯含量显著高于10%和15%组。10%和15%组的血浆尿素氮含量显著高于其他实验组。15%和20%组的血细胞数量显著高于其他实验组。30%组的细胞凋亡率及细胞内活性氧(ROS)和钙离子(Ca2+)含量显著高于其他实验组。饲料糊精水平对暗纹东方鲀幼鱼的血糖含量、血浆谷草转氨酶和谷丙转氨酶活性及肝胰脏和肠道中脂肪酶活性无显著影响。在本实验条件下,暗纹东方鲀幼鱼饲料中糊精的适宜水平为20%~25%。
英文摘要:
      A feeding trial was conducted to determine the effect of dietary dextrin level on growth,activities of digestive enzyme and blood biochemical indices of juvenile obscure puffer,Takifugu obscurus.Five iso-nitrogenous(42%)and iso-lipidic(9%)diets containing graded levels of dextrin(10%,15%,20%,25% and 30%)were prepared.The fish[average weight(10.25±0.51 g)]were stocked in triplicate tanks during a feeding period of 60 days.The results showed that best specific growth rate and feed efficiency occurred for fish fed the diet with dietary dextrin level at 25%.Viscerosomatic index and hepatosomatic index of 30% group were significantly higher than those of 10% group(P<0.05),meanwhile liver lipid content of 30% group was also significantly higher than other groups(P<0.05).Liver glycogen and total amino acids content in plasma of 10% group were significantly lower than those of other groups(P<0.05).Compared with the 10% and 15% groups,amylase activity in hepatopancreas and intestine of other groups was significantly increased(P<0.05),while trypsin activity in hepatopancreas of the 30% group decreased significantly(P<0.05); total cholesterol and triglycerides content in plasma of the 25% and 30% groups increased significantly(P<0.05),while blood urea nitrogen in plasma of other groups decreased significantly(P<0.05).Red blood cell counts of 15% and 20% groups were significantly higher than other those of groups(P<0.05),while apoptosis ratio,reactive oxygen species and calcium ions content of 30% group were significantly higher than those of other groups(P<0.05).However,plasma glucose concentration,aspartate aminotransferase and alanine aminotransferase activity as well as lipase activity in hepatopancreas and intestine of obscure puffer were not affected by dietary dextrin levels.The present results showed that the optimum dextrin level in diets is at 20% -25% for juvenile obscure puffer under the present research conditions.
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