文章摘要
朱其建,戴习林,邹卫丽,蒋 飞,苏 建,过正乾,王海洋,丁福江.罗氏沼虾抗病选育群体的抗病性能及其遗传多样性分析[J].水产学报,2013,37(10):1468~1478
罗氏沼虾抗病选育群体的抗病性能及其遗传多样性分析
Disease resistance and genetic diversity analysis in selected populations of Macrobrachium rosenbergii
投稿时间:2013-04-10  修订日期:2013-06-14
DOI:10.3724/SP.J.1231.2013.38664
中文关键词: 罗氏沼虾  选育  抗病力  微卫星  遗传多样性
英文关键词: Macrobrachium rosenbergii  selective breeding  disease resistance  microsatellite marker  genetic diversity
基金项目:科技部科技人员服务企业行动项目(2009GJC00021);上海市科委重点攻关项目(11391901400);上海市教育委员会重点学科建设项目(J50701)
作者单位
朱其建 上海海洋大学水产与生命学院 
戴习林 上海海洋大学水产与生命学院 
邹卫丽 上海海洋大学水产与生命学院 
蒋 飞 上海海洋大学水产与生命学院 
苏 建 上海海洋大学水产与生命学院 
过正乾 上海海洋大学水产与生命学院 
王海洋 上海海洋大学水产与生命学院 
丁福江 上海申漕特种水产开发公司 
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中文摘要:
      为深入了解罗氏沼虾抗病选育群体的抗病力和遗传信息,通过人工注射溶藻弧菌感染16个罗氏沼虾选育群体,并利用微卫星分子标记对选育群体进行遗传结构分析。结果显示,16个选育群体抗溶藻弧菌感染能力存在明显差别,并从中鉴定出抗病力强的群体3个(SCR11-6、SCR11-11和SCR11-16),其在感染溶藻弧菌感染后的成活率达80%;抗病力比较强的群体7个;抗病力一般的群体4个,成活率为65%~70%;抗病力差的群体2个,成活率为35%~50%。8对微卫星引物共检测到53个等位基因,每对引物的等位基因数为5~9个,平均为6.625个,多态信息含量(PIC)为0.629 4~0.829 4,平均为0.732 3。16个群体的平均PIC为0.493 2~0.695 6,平均观测杂合度为0.506 2~0.651 3,平均期望杂合度为0.551 9~0.733 2,遗传相似系数平均为0.655 2,遗传距离平均为0.434 4。并对DP和SP两群体罗氏沼虾个体扩增出的差异条带进行统计,分析其与罗氏沼虾抗病性状的相关性。结果表明,5个微卫星位点SUGbp8-103b、SUGbp8-101c、MRMB11、SUGbp8-137和MRMC2分别在203、263、185、335和96 bp等位基因与罗氏沼虾抗病性状存在一定的相关性,其中,位点MRMB11在185 bp等位基因跟抗病性有极显著的正相关性,相关系数为0.282。研究表明,16个选育群体中有4个群体的抗病力较强,同时与其它12个选育群体相比,这4个群体遗传多样性也比较丰富,这些罗氏沼虾群体的筛选为罗氏沼虾抗病新品种的培育奠定了重要基础。
英文摘要:
      16 selected populations were injected with bacteria(Vibrio alginolyticus)suspension to know deeply about disease resistance of Macrobrachium rosenbergii.Bacterial challenge experiments demonstrated that there were big differences in disease resistance among the 16 selected populations.Some families exhibited strong disease resistance to bacterial infection,while some families were not resistant to bacterial challenge.Among the 16 selected populations,3 populations(population SCR11-6,SCR11-11 and SCR11-16)with strong disease resistance with a survival rate of 80% were identified,7 populations with secondly strong disease resistance,4 populations with normal disease resistance with a survival rate of 65%-70%,and 2 populations with low disease resistance with a survival rate of 49.53% and 35.0% were identified.The genetic structure of 16 selected populations was analyzed assessed by 8 microsatellite markers.The results showed that the number of total alleles was 53.The numbers of allele at each locus ranged from 5 to 9 with an average of 6.625,and polymorphic information content(PIC)was 0.629 4-0.829 4,and the average was 0.732 3.The mean polymorphism information content in 16 selected populations was 0.493 2-0.695 6.The average observed and expected heterozygosity of the 16 selected populations varied from 0.506 2 to 0.651 3 and from 0.551 9 to 0.733 2,respectively,and the average genetic similarity coefficient and genetic distance between them was 0.655 2 and 0.434 4,respectively.Besides,after PCR amplification on the individuals of DP and SP populations,differential bands were calculated and the correlation between growth trait and microsatellite loci was established.The results showed that 5 loci were correlated with disease resistant trait,of which MRMB11 had extreme significance of 0.282.These results indicated that 4 of 16 selected populations had strong disease resistance and high genetic diversity.The development of these 8 populations lays foundation for selective breeding of M.rosenbergii with disease resistance.
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