文章摘要
戴 超,王 芳,房子恒,董双林.温度对蜕壳后期三疣梭子蟹能量代谢酶活力的影响[J].水产学报,2013,37(9):1334~1341
温度对蜕壳后期三疣梭子蟹能量代谢酶活力的影响
Effects of temperature on energy metabolic enzymes of swimming crab (Portunus trituberculatus)in the post-molt stage
投稿时间:2013-04-15  修订日期:2013-05-02
DOI:DOI:10.3724/SP.J.1231.2013.38676
中文关键词: 三疣梭子蟹  钙化  己糖激酶  丙酮酸激酶  琥珀酸脱氢酶
英文关键词: Portunus trituberculatus  calcification process  hexokinase(HK)  pyruvate kinase(PK)  succinate dehydrogenase(SDH)
基金项目:国家“十二五”科技支撑计划项目(2011BAD13B03)
作者单位
戴 超 中国海洋大学水产学院,教育部海水养殖重点实验室 
王 芳 中国海洋大学水产学院,教育部海水养殖重点实验室 
房子恒 中国海洋大学水产学院,教育部海水养殖重点实验室 
董双林 中国海洋大学水产学院,教育部海水养殖重点实验室 
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中文摘要:
      为研究温度对三疣梭子蟹雌蟹钙化过程中丙酮酸激酶(PK)、己糖激酶(HK)和琥珀酸脱氢酶(SDH)活力的影响,在实验室条件下设18、22、26 ℃ 3 个温度水平,分别在梭子蟹蜕壳后1、2、8、16 h及完全硬化5个时间点取样,测定其肝胰脏、肌肉和鳃中HK、PK、SDH的活力。结果显示:①与22 ℃条件下相比,高温(26 ℃)和低温(18 ℃)下三疣梭子蟹肌肉中PK的活力在蜕壳后1~8 h明显升高,说明高温和低温条件下梭子蟹肌肉中的糖酵解速率加大;而肝胰脏和鳃中的PK活力的峰值出现在梭子蟹蜕壳后1~2 h,表明蜕壳后1~2 h这2种组织中有较大的糖酵解速率;②3个温度水平下,梭子蟹肝胰脏中HK的活力随钙化过程的进行而降低,蜕壳后1~16 h的活力显著高于完全钙化时的活力,说明蜕壳后的梭子蟹肝胰脏对葡萄糖的利用能力逐渐减弱,而其鳃中HK活力的变化趋势与肝胰脏中相反,说明梭子蟹的鳃对葡萄糖的利用能力随钙化过程的进行而提高,这种差异可能与不同组织在梭子蟹钙化过程中对葡萄糖的利用能力不同有关;③梭子蟹肝胰脏中SDH的活力在3个实验温度下蜕壳后16 h内呈逐渐升高的趋势,说明肝胰脏在钙化过程中三羧酸循环作用逐渐加强。此外,其肌肉和鳃组织中SDH的活力在3个实验温度下的峰值均出现在前8小时,并且低温(18 ℃)的峰值明显低于高温(26 ℃),说明在这段钙化时间中,相对于低温而言,梭子蟹的肌肉和鳃中有氧代谢的加强在高温条件下更显著。在钙化过程中,低温条件下三疣梭子蟹的糖酵解作用没有明显的变化,而三羧酸循环有较明显的减弱趋势,其中三疣梭子蟹肌肉和鳃中SDH的活力明显低于高温条件,表明低温确实可以延长其蜕壳后的软壳时间。
英文摘要:
      The present work was carried out to study the effect of temperature on some metabolic enzyme activities in the female swimming crab(Portunus trituberculatus)during its calcification process in laboratory.Activities of PK,HK and SDH were measured in hepatic,muscular and branchial tissue of the swimming crab at 1,2,8,16 h after molting and the completely hardening stage at different temperatures(18 ℃,22 ℃,26 ℃),respectively.The primary results are recorded as belows:①Compared to moderate temperature(22 ℃),PK activity in muscle of P.trituberculatus was found to be significantly increased during 1-8 h after molting at higher temperature(26 ℃)and lower temperature(18 ℃),indicating that the glycolysis rate increased in muscular tissue of the swimming crab at higher and lower temperatures; PK dynamics was found to be peaked in hepatic and branchial tissues of P.trituberculatus at 1-2 h after molting which showed a greater rate of glycolysis in these two tissues.②HK activity in the hepatic tissue of P.trituberculatus decreased during the calcification process in all treatments.HK activity in the liver at 1-16 h was significantly higher than the activity of calcified status,indicating a gradually decreased utilizing capacity of glucose in liver of P.trituberculatus during the calcification process.However,the branchial HK activities showed an opposite dynamic trend compared with that in hepatic tissue,which projected an increased utilizing capacity of glucose levels in gills of P.trituberculatus during the calcification process.The difference may be related to different capabilities to use glucose in different tissues of the swimming crab during calcification process.③SDH activity in hepatic tissue of P.trituberculatus in the all treatments was found to be gradually increased within 16h after molting,indicating that a gradual strengthening of the citric acid cycle in the liver during calcification process.In addition,SDH dynamics was found to be peaked in muscular and branchial tissues of crab occurring within 8h after molting in the all treatments.The activity of SDH at low temperature(18 ℃)was significantly lower than that at high temperature(26 ℃).The statistics described above showed an aerobic strengthening in musclar and branchial tissues of P.trituberculatus at high temperature compared to the low temperature.In summary,glycolysis process under low temperature conditions did not change significantly,while the citric acid cycle had been significantly weakened during the calcification process.The activity of SDH in muscle and branchial tissues of P.trituberculatus at lower temperature was significantly lower than that at higher temperature,indicating that lower temperature can indeed extend the time of its soft-shell stage after molting.
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