文章摘要
毛芝娟,毛甬州,汪建萍.两株鱼源琼氏不动杆菌的分离、鉴定和耐药特性分析[J].水产学报,2013,37(10):1572~1578
两株鱼源琼氏不动杆菌的分离、鉴定和耐药特性分析
Isolation,identification and drug-resistance genes detection of Acinetobacter junii from fish
投稿时间:2013-05-02  修订日期:2013-07-17
DOI:10.3724/SP.J.1231.2013.38707
中文关键词: 琼氏不动杆菌  致病性  鉴定  耐药基因
英文关键词: Acinetobacter junii  pathogenicity  species identification  drug-resistance genes
基金项目:浙江省科技厅重点科技创新团队项目(2012R10025)
作者单位
毛芝娟 浙江万里学院生物与环境学院 
毛甬州 浙江万里学院生物与环境学院 
汪建萍 浙江万里学院生物与环境学院 
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中文摘要:
      为探明史氏鲟感染性疾病的病原,并为生产中防治该病提供依据,本实验自患病鱼分离到2株细菌SX01和SX02,对2分离株开展了人工感染实验、形态学和理化特性检测及分子生物学鉴定,并用PCR法检测了部分耐药基因。研究结果表明,分离株对异育银鲫具有一定的致病力,96 h的半数致死浓度LD50为1.02×109 cells/mL;革兰氏染色阴性,球杆状,氧化酶实验、动力阴性,接触酶阳性,形态学和理化特性符合不动杆菌属的特征;其16S rRNA基因序列与已发表的琼氏不动杆菌完全同源,RNA聚合酶β亚单位编码基因rpoB序列与琼氏不动杆菌相应序列同源性达99%以上,确认2分离株为琼氏不动杆菌;自分离株中检测到了广谱性β-内酰胺酶编码基因TEM-1和氨基糖苷类乙酰胺转移酶基因Aac(3)Ⅱ,解释了该菌对2类抗生素药物的耐受性,提示了该菌可能来源于临床菌的污染,养殖行业和食品卫生中必须引起重视。
英文摘要:
      In this study,two bacteria strains SX01 and SX02 were isolated from diseased Acipenser schrenckii and the carps(Carassais auratus gibelio)were artificially challenged to test the pathogenicity of the strains.Morphology profiles were observed,physical and biochemical characteristics were tested,and molecular analysis was combined to identify the isolated strains,also antibiotics drug resistance genes were detected.The results showed,the strains were pathogenic to the carps,with the 96 h LD50=1.02×109cells/mL.The strains were Gram negative,short rods,nearly sphere,oxidation test and motility negative,catalase positive,showed the profiles of Acinetobacter genus.16S rRNA gene sequence of the strains shared 100% identity with those published data of A.junii in GenBank,and rpoB sequence of SX01 showed 99% identity with those of A.junii,were well identified the strains to A.junii.A β-lactam enzyme coding gene TEM-1 and acetamide transferase gene Aac(3)Ⅱ were detected in the strains,suggesting possible clinical contamination.Enough attention must be paid to guard against the drug-resistant A.junii in aquaculture and food sanitation.
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