文章摘要
季晓芬,汪登强.基于微卫星评估草鱼放流亲本对野生群体遗传多样性的影响[J].水产学报,2018,42(1):10~17
基于微卫星评估草鱼放流亲本对野生群体遗传多样性的影响
Genetic effect of released brood grass carp on wild population in the Yangtze River inferred from microsatellite markers
投稿时间:2016-02-17  修订日期:2017-04-10
DOI:10.11964/jfc.20160210286
中文关键词: 草鱼  微卫星  遗传多样性  增殖放流
英文关键词: grass carp  microsatellite  genetic diversity  releasing
基金项目:国家自然科学基金
作者单位E-mail
季晓芬 中国水产科学研究院长江水产研究所 503062413@qq.com 
汪登强 中国水产科学研究院长江水产研究所 wdq@yfi.ac.cn 
摘要点击次数: 1040
全文下载次数: 1004
中文摘要:
      利用筛选的13对草鱼多态性微卫星标记,开展了2011至2015年长江中游草鱼亲本增殖放流对野生群体遗传多样性的影响评估。结果表明这13个微卫星位点的多态信息含量为0.8622(0.657~0.950),基因多样度为0.8555(0.675~0.936)。15个群体的有效等位基因数分别为7.4503~10.1536,等位基因丰度为11.483~15.204,说明15个草鱼群体的遗传多样性水平总体较高。遗传分化指数分析表明,群体间不存在显著遗传分化(FST < 5%)。通过贝叶斯聚类分析和主成分分析可将草鱼群体分为4个组群,根据分组结果以及来源划分分别对草鱼群体进行AMOVA 分析,结果表明,遗传变异大部分来自于群体内个体间,组间及组内群体间的分化水平较低(FCT < 5%;FSC < 5%),与FST分析结果一致。综上所述, 当前草鱼亲本增殖放流模式对野生群体遗传结构影响不明显。
英文摘要:
      The natural resource of grass carp (Ctenopharyngodon idellus) in Yangtze River has significantly decreased in the past decades. In recent years, large-scale releases of brood grass carp are carried out for ecological restoration in Yangtze River. In order to assess the genetic effect of released brood fishes on wild population in the middle reach of Yangtze River, thirteen polymorphism microsatellite markers were selected to genotype the genetic differentiation among released and wild populations. Fifteen populations were sampled, including ten released and five wild populations. The results showed that polymorphic information content and gene diversity of the microsatellite loci was 0.8622 (0.657~0.950) and 0.8555 (0.675~0.936), respectively. The number of effective alleles of the 15 populations was ranged from 7.4503 to 10.1536, and allelic richness from 11.483 to 15.204, which indicated that genetic diversity of grass carp populations was moderately high. The total and pairwise genetic differentiation indexes (FST) were low to 5%, loci implying that there was no significant genetic differentiation among populations. The 15 grass carp populations were divided into 4 groups based on Bayesian clustering analysis and principal component analysis. According to above grouping and geographic?distribution of the 15 grass carp populations, we have conducted AMOVA analysis, as a result, most of the genetic variation of grass crap populations was from inter-individuals, the level of genetic?differentiation among groups was low(FCT < 5%; FSC < 5%). In summary, there was hardly genetic effect of releasing nature grass carp on wild populations in the Yangtze River under current model.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看