文章摘要
彭爱红,高爽,陈俊,庄梅珍,王凡,陈晓梅,林郑忠,黄志勇.锌胁迫对海洋小球藻生长和金属硫蛋白诱导的影响[J].水产学报,2017,41(7):1126~1132
锌胁迫对海洋小球藻生长和金属硫蛋白诱导的影响
Growth exhibition and metallothioneins induction of Chlorella sp. exposed to different zinc salts
投稿时间:2016-04-10  修订日期:2017-01-15
DOI:10.11964/jfc.20160410349
中文关键词: 海洋小球藻  锌胁迫  葡萄糖酸锌  生物量  金属硫蛋白
英文关键词: Chlorella sp.  zinc stress  zinc gluconate  biomass  metallothioneins
基金项目:福建省自然科学基金(2015J01615);厦门市海洋经济发展专项(14GZP67NF31);集美大学创新团队基金(2010A007)
作者单位E-mail
彭爱红 集美大学食品与生物工程学院, 福建 厦门 361021  
高爽 集美大学食品与生物工程学院, 福建 厦门 361021  
陈俊 集美大学食品与生物工程学院, 福建 厦门 361021  
庄梅珍 集美大学食品与生物工程学院, 福建 厦门 361021  
王凡 集美大学食品与生物工程学院, 福建 厦门 361021  
陈晓梅 集美大学食品与生物工程学院, 福建 厦门 361021  
林郑忠 集美大学食品与生物工程学院, 福建 厦门 361021  
黄志勇 集美大学食品与生物工程学院, 福建 厦门 361021 zhyhuang@jmu.edu.cn 
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中文摘要:
      研究锌离子胁迫对海洋小球藻生长和金属硫蛋白诱导的影响,当小球藻达到对数生长期,用不同离子浓度(5、10、20、50和100 μmol/L)的锌盐(氯化锌、柠檬酸锌、乙酸锌和葡萄糖酸锌)分别进行胁迫培养,通过测定小球藻的生物量和锌结合金属硫蛋白含量,考察锌胁迫对海洋小球藻的生物量、热稳定蛋白含量以及锌结合金属硫蛋白含量的影响。结果显示,当浓度分别小于5和10 μmol/L时,柠檬酸锌和氯化锌对小球藻生长无显著抑制作用;而浓度为5 μmol/L时,乙酸锌和葡萄糖酸锌对小球藻生长即产生显著抑制,且抑制作用皆随锌离子浓度的增加而增大。与对照组相比,4种锌盐胁迫3 d后小球藻产生的热稳定蛋白含量极显著增加,其中以锌离子浓度为50 μmol/L的葡萄糖酸锌对小球藻胁迫产生的热稳定蛋白含量最高,达到34.5 mg/g(湿藻泥)。同样在此浓度的葡萄糖酸锌胁迫下,小球藻诱导产生的锌结合金属硫蛋白的含量最高。研究表明,用锌离子浓度为50 μmol/L的葡萄糖酸锌对小球藻胁迫培养,诱导产生的锌结合金属硫蛋白含量达到最高。
英文摘要:
      The exhibition of growth and the induction of metallothioneins of Chlorella sp. exposed to different kinds of zinc salts were investigated. The biomass, heat-stable proteins and Zn-MTs were measured when the Chlorella sp. were exposed to four kinds of Zn2+ (including zinc chloride, zinc acetate, zinc gluconate, zinc citrate) at the concentrations of 5, 10, 20, 50 and 100 μmol/L. Results showed that the growth of Chlorella sp. was not significantly inhibited when the zinc concentrations were less than 5 and 10 μmol/L for zinc citrate and zinc chloride, respectively. But the growth was significantly inhibited at the concentrations of 5 μmol/L for each of zinc acetate and zinc gluconate, and the algal biomass rapidly decreased with the increases of Zn2+ concentrations for the two zinc salts. The results also showed that the amounts of heat-stable proteins significantly increased in Chlorella sp. exposed to the four kinds of zinc salts compared with that of the control group (without adding any zinc salt), and the largest amount of heat-stable proteins was obtained when the algae were exposed to zinc gluconate at 50 μmol/L for 3 days, producing 34.5 mg of heat-stable proteins per gram of wet algae mud, which was 3.3 times higher than that of the control group. And the largest amount of Zn-MTs was induced under the inducing condition.
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