文章摘要
徐湛宁,李福贵,郑国栋,陈杰,蒋霞云,邹曙明.团头鲂耐低氧新品系雌核发育群体遗传结构的微卫星分析[J].水产学报,2017,41(3):330~338
团头鲂耐低氧新品系雌核发育群体遗传结构的微卫星分析
Analysis of genetic structure of gynogenetic population in new strain of hypoxia-tolerant Megalobrama amblycephala using microsatellite markers
投稿时间:2016-04-25  修订日期:2016-08-13
DOI:10.11964/jfc.20160410379
中文关键词: 团头鲂  耐低氧群体  雌核发育  遗传结构  微卫星标记
英文关键词: Megalobrama amblycephala  hypoxia-tolerant  gynogenesis  genetic structure  microsatellite
基金项目:国家科技支撑计划(2012BAD26B00);国家自然科学基金(31272633,31201760);上海高校知识服务平台(ZF1206)
作者单位E-mail
徐湛宁 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306  
李福贵 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306  
郑国栋 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306  
陈杰 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306  
蒋霞云 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306  
邹曙明 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306 smzou@shou.edu.cn 
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中文摘要:
      为了指导团头鲂耐低氧群体的后续选育工作开展,利用筛选出的20对微卫星引物,比较分析了团头鲂耐低氧群体(TN)及其减数分裂(TNM)、有丝分裂(TNDH)雌核发育后代群体的遗传结构;结果显示,团头鲂TN、TNM、TNDH及团头鲂“浦江1号”(TPJ1)对照的平均等位基因数(Na)分别为3.90、3.55、3.45、4.25,平均观测杂合度(Ho)分别为0.7853、0.3934、0.2768、0.8075,平均期望杂合度(He)分别为0.6491、0.5563、0.4870、0.6855,平均多态信息含量(PIC)分别为0.5695、0.4796、0.4181、0.6105,TPJ1对照群体的遗传多样性最高,TN群体较TPJ1群体的遗传多样性有所降低,但不存在显著差异,仍保持了较高的遗传杂合度,而2个雌核发育群体(TNM和TNDH)的遗传多样性显著低于TPJ1和TN群体,TNDH群体的遗传多样性显著低于TNM群体。Hardy-Weinberg平衡遗传偏离指数也表明团头鲂TPJ1和TN群体出现杂合子过剩现象,而TNM、TNDH雌核发育群体则出现了杂合子缺失现象,纯合子比例高。聚类分析表明,TPJ1和TN群体聚类成一个分支,而TNM、TNDH雌核发育群体聚类成另一分支,产生了一定程度的遗传分化。在TN群体中实施雌核发育可加速遗传物质的纯合,可用于团头鲂进一步耐低氧性状基因的纯化。
英文摘要:
      To guide the subsequent breeding of hypoxia-tolerant population of Megalobrama amblycephala; genetic structures of hypoxia-tolerant (TN), meiotic gynogenesis (TNM), mitotic gynogenesis populations (TNDH) of M. amblycephala were analyzed using twenty microsatellite markers in this study. The mean value of number of alleles (Na) of M. amblycephala TN, TNM, TNDH and the control TPJ1 was 3.90, 3.55, 3.45 and 2.56, respectively. The mean value of observed heterozygosity (Ho) was 0.7853, 0.3934, 0.2768 and 0.8075, respectively. The mean value of expected heterozygosity (He) was 0.6491, 0.5563, 0.4870 and 0.6855, respectively. And the average value of polymorphism information content (PIC) was 0.5695, 0.4796, 0.4181 and 0.6105, respectively. It demonstrated that the genetic diversity of TPJ1 was the highest, and TN population had lower genetic diversity, but there is no significant difference and there was the high genetic heterozygosity. The genetic diversity of TNDH population was significantly lower than TNM population, and the genetic diversity of the gynogenetic (TNM and TNDH) was significantly lower than TPJ1 and TN populations. The mean Hardy-Weinberg index of the TPJ1 and TN had heterozygote excess and the gynogenetic populations (TNM and TNDH) showed heterozygote deficit. Using unweighted pair-group method with arithmetic means method (UPGMA) based on their genetic distances, TPJ1 population and TN population were grouped in one cluster, while TNM population and TNDH population were classified into another cluster, indicating a genetic differentiation between the two clusters. Our results show that the hypoxia-tolerant group in M. amblycephala (TN) in gynogenesis can accelerate homozygosity of genetic material and will have further purification of M. amblycephala hypoxia-tolerant genes.
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