文章摘要
周伟,高天翔,王俊,宋娜.乌鳢群体遗传多样性和遗传结构分析[J].水产学报,2017,41(10):1521~1532
乌鳢群体遗传多样性和遗传结构分析
Genetic diversity and structure analysis based on the mitochondrial DNA control region of the northern snakehead (Channa argus)
投稿时间:2016-05-19  修订日期:2016-10-15
DOI:10.11964/jfc.20160510409
中文关键词: 乌鳢|mtDNA控制区|遗传多样性|遗传结构|群体历史动态
英文关键词: Channa argus|mtDNA control region|genetic diversity|genetic structure|population demography
基金项目:公益性行业(农业)科研专项(201303050)
作者单位E-mail
周伟 中国海洋大学水产学院, 山东 青岛 266003  
高天翔 浙江海洋大学水产学院, 浙江 舟山 316022  
王俊 中国水产科学研究院黄海水产研究所, 山东 青岛 266071  
宋娜 中国海洋大学水产学院, 山东 青岛 266003 songna624@163.com 
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中文摘要:
      为了解乌鳢群体遗传变异规律,本研究对8个群体共212个个体的mtDNA控制区全序列进行群体遗传多样性、遗传结构和群体历史动态分析。结果显示,乌鳢控制区全序列长度为907 bp,乌鳢群体单倍型多样性水平变化较大,中国黄河及以北的乌鳢群体单倍型多样性水平比淮河和长江等南方群体相对较低,所有群体表现出较低的核苷酸多样性水平(h<0.5%)。基于单倍型构建的系统发育树和群体聚类树结果均未显示出与地理位置相对应的谱系结构。单倍型网络图显示存在多个主单倍型。遗传结构分析显示,不同水系间存在显著的遗传差异,相同水系间遗传差异较小。群体历史动态分析显示,中国乌鳢所有群体有效种群数量在中更新世晚期到晚更新世0.222—0.050百万年出现了一次较明显的快速增长,之后在晚更新世末次冰期0.050—0.010百万年出现了有效种群下降,伴随着全新世到来,在0.010百万年之后,乌鳢群体又发生了一次较小的有效种群增长。洞庭湖群体则发生一次有效种群的快速增长,增长时间大约在0.160百万年。研究表明,青藏高原隆起后,东亚季风在中国南北方气候的差异和秦岭山脉屏障对季风的阻断作用加大了这一差异,可能对乌鳢群体的遗传多样度差异造成一定影响。乌鳢群体不存在显著的谱系结构可能与乌鳢遗传分化时间较短有关,但是地理隔离等因素导致了不同水系间确切的遗传差异。第四纪更新世气候的波动,尤其是中更新世间冰期气候的转暖、末次盛冰期的降温和冰后期全新世的到来可能对乌鳢群体的数量和栖息地的扩缩起着重要影响。
英文摘要:
      The northern snakehead (Channa argus) is extensively distributed throughout rivers and lakes of China. With overexploitation and water eco-environment deterioration, the resources of C. argus are severely affected. To examine genetic structure and the demographic history of the northern snakehead, we collected 212 specimens from 8 locations for generating mitochondrial DNA complete control region sequences. Result showed that the length of control region is about 907 bp. The genetic diversity in southern China is higher than that in the north with boundary line 34°N. The factors that warm and wet climate in the south China and dry and cold climate in the north China, and that the rapid uplift of Qinling Mountain aggravated differentiation of the east Asian monsoon, may have an important influence on genetic diversity. The phylogenetic tree showed a shallow genealogy and high genetic differentiation and significant level of genetic structure which behaved in different drainages. This indicated that geographical barriers led to recent genetic differentiation. The demographic history of 7 populations of China displayed that the northern snakehead experienced population rapid growth in the interglacial period of the middle Quaternary. Then, the demography declined during the last glacial period. With climatic warming in the Holocene, a slight population growth happened. The Dongting Lake population also experienced a population expansion in the interglacial period of the middle Quaternary. The glacial-interglacial cycles in the Quaternary, especially from the middle Quaternary, caused pronounced climatic fluctuations and had an important influence on the northern snakehead populations. Information on genetic diversity and genetic structure will have implications for the management of fisheries and conservation efforts.
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