文章摘要
施慧,谢建军,汪玮,王庚申,何杰,许文军.养殖大黄鱼“白鳃病”一种新病毒病原的初步研究[J].水产学报,2017,41(9):1455~1463
养殖大黄鱼“白鳃病”一种新病毒病原的初步研究
Preliminary study of a new virus pathogen that caused the white-gill disease in cultured Larimichthys crocea
投稿时间:2016-06-06  修订日期:2017-01-13
DOI:10.11964/jfc.20160610430
中文关键词: 大黄鱼  白鳃病  真鲷虹彩病毒  组织病理学  聚合酶链式反应
英文关键词: Larimichthys crocea  white-gill  red sea bream iridovirus virus  histopathology  polymerase chain reaction
基金项目:浙江省自然科学基金(Y15C190018);浙江省科技厅院所专项(2015F30003;2015F10001);浙江海洋大学博士启动基金(22135010815);浙江海洋大学“水产”省一流学科开放课题(20160008)
作者单位E-mail
施慧 浙江省海洋水产研究所 浙江省海水增养殖重点实验室, 浙江 舟山 316100  
谢建军 浙江省海洋水产研究所 浙江省海水增养殖重点实验室, 浙江 舟山 316100  
汪玮 浙江省海洋水产研究所 浙江省海水增养殖重点实验室, 浙江 舟山 316100  
王庚申 浙江省海洋水产研究所 浙江省海水增养殖重点实验室, 浙江 舟山 316100  
何杰 浙江省海洋水产研究所 浙江省海水增养殖重点实验室, 浙江 舟山 316100  
许文军 浙江海洋大学渔业学院, 浙江 舟山 316100 xwenjun@sina.com 
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中文摘要:
      运用组织病理学、电镜观察及PCR扩增等方法对近年来网箱养殖大黄鱼暴发的“白鳃病”进行了研究,以探讨引起养殖大黄鱼“白鳃”关联病毒的致病机理。临床解剖观察显示:患“白鳃病”的鱼表现出极度的贫血症状、鳃丝苍白色、血液稀薄且血细胞数显著减少;内脏组织病理观察结果显示:鱼的肝、脾、肾脏内脏组织发生严重的病理变化,组织内红细胞发生明显的退行性变化,同时鱼体的红细胞数量显著减少;采用针对真鲷虹彩病毒(red sea bream iridoviral virus,RSIV)的引物对病鱼内脏组织核酸样本进行PCR检测,结果显示,患“白鳃病”病鱼样本RSIV核酸呈阴性;组织超薄切片电镜观察结果显示:在患“白鳃病”病鱼脾脏和肾脏组织细胞的胞质中可见直径约40~45 nm的病毒粒子。由此初步判断,浙江地区网箱养殖大黄鱼的“白鳃病”不是由RSIV导致,而与一种直径为45~50 nm的病毒有直接关联,本研究为大黄鱼疾病的诊断与防治提供了参考依据。
英文摘要:
      By using histopathology and polymerase chain reaction (PCR) methods, studies were carried out to find the cause of the white-gill disease in Larimichthys crocea cultured in cage in Zhejiang Province. White-gill diseased fish had the typical symptom of pale gills and there is no ulcer in the body surface of infected fish. However, the blood and blood cell counts in the white-gill disease fish were significantly decreased. Pathological observation showed serious pathological changes in internal organs. In white-gill diseased fish, haemocytes, in particular, erythrocytes reduced sharply in the blood vessels of liver, spleen and other organs and degenerative changes occurred in red blood cells, too. Under electron microscope, a large number of viral particles were observed in the cytoplasm of spleen, with an average diameter of 40 to 45 nm. With the primers derived from RSIV, no fragments of the RSIV DNA could be amplified from the white-gill diseased fish. The results indicated that although white-gill disease and RSIVD both can lead to the symptom of pale gills, but the pathogen that caused each disease was completely different, and the white-gill disease that broke out in Zhejiang may be associated with another virus.
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