文章摘要
解伟,郭冉,夏辉,王小瑞,李赵嘉,李垚垚,王美雪,沈庆洲.黄曲霉毒素B1对凡纳滨对虾幼虾肝胰腺抗氧化酶的损伤机制[J].水产学报,2017,41(3):448~455
黄曲霉毒素B1对凡纳滨对虾幼虾肝胰腺抗氧化酶的损伤机制
Damage mechanism of aflatoxin B1 on antioxidant enzyme in hepatopancreas of juvenile Litopenaeus vannamei
投稿时间:2016-06-07  修订日期:2016-09-12
DOI:10.11964/jfc.20160610438
中文关键词: 凡纳滨对虾  黄曲霉毒素B1  抗氧化酶
英文关键词: Litopenaeus vannamei  aflatoxin B1  antioxidant enzyme
基金项目:国家自然科学基金(31202010);河北省科技支撑项目(14967117D)
作者单位E-mail
解伟 河北农业大学海洋学院, 河北 秦皇岛 066003  
郭冉 河北农业大学海洋学院, 河北 秦皇岛 066003 toguoran@163.com 
夏辉 河北农业大学海洋学院, 河北 秦皇岛 066003  
王小瑞 河北农业大学海洋学院, 河北 秦皇岛 066003  
李赵嘉 河北农业大学海洋学院, 河北 秦皇岛 066003  
李垚垚 河北农业大学海洋学院, 河北 秦皇岛 066003  
王美雪 河北农业大学海洋学院, 河北 秦皇岛 066003  
沈庆洲 河北农业大学海洋学院, 河北 秦皇岛 066003  
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中文摘要:
      为考察黄曲霉毒素B1对凡纳滨对虾幼虾抗氧化酶的损伤机制,选取体质量为(0.3±0.02)g的凡纳滨对虾进行实验,实验组饲喂含2000 μg/kg AFB1的饲料,对照组饲喂无AFB1饲料,每组设3个平行,共养殖4周,每隔4天进行1次取样,测定肝胰腺抗氧化酶指标。结果显示,幼虾在摄入高浓度AFB1后,第8天实验组体内SOD显著低于对照组,连续饲喂2周后机体内的SOD活性显著下降,实验组酶活性极显著低于对照组;同时CAT活性随着时间的推移呈现下降的趋势,且每个阶段之间差异显著,20 d后虽有上升趋势,但与初始相比显著降低,第8天之后,实验组CAT活性均低于对照组,在第8、16和24天酶活性极显著低于对照组。GSH-Px活性整体均低于对照组,且机体自我修复能力下降。同时脂质过氧化物MDA的含量在16 d后出现显著增加,同时实验组MDA含量极显著高于对照组,且继续呈现增加趋势。研究表明高浓度AFB1对凡纳滨对虾抗氧化酶的影响因酶种类而异,同时对肝胰腺的损伤持续增加,在4周内便会造成肝胰腺的严重损伤。
英文摘要:
      Some antioxidant enzymes in hepatopancreas were examined to evaluate the damage of the dietary AFB1 on antioxidant enzyme in hepatopancreas of juvenile Litopenaeus vannamei. Two kinds of diets (control diet and test diet containing 2000 μg/kg of aflatoxin B1) were fed to the shrimp[with body weight (0.3±0.02) g] for 4 weeks. 420 shrimps were selected randomly to 6 aquariums (40 cm ×50 cm ×60 cm).The activity of some antioxidant enzymes in hepatopancreas were determined every four days. The results showed that the activity of SOD of test group was significantly lower than that in control group on the 8th day. In two weeks the activity of SOD decreased significantly, and the activity of test group was significantly lower than that in control group. The activity of CAT significantly decreased in each stage. After 8 days, the activity of CAT in test group was lower than control group, especially, the activity of test group was significantly lower than control group on the 8th, 16th and 24th days. Although there was a rising trend after 20 days, the CAT activity showed significant decline from the original value.The activity of GSH-Px was lower than that in the control group, with the ability of repairing themselves decreasing. The content of MDA increased significantly after 16 days and there was a rising trend. Meanwhile, the content of MDA in test group was significantly higher than that in control group. These results indicate that the damage of high concentrations of AFB1 on antioxidant enzyme in L. vannamei varies with different enzymes, and the damage on hepatopancreas continues to increase, causing severe hepatopancreas damage only in 4 weeks.
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