文章摘要
卓丽欣,赵红霞,黄燕华,曹俊明,王国霞,陈 冰,孙育平.氧化鱼油对黄颡鱼幼鱼肠道健康的影响及精氨酸的干预作用[J].水产学报,2018,42(1):100~111
氧化鱼油对黄颡鱼幼鱼肠道健康的影响及精氨酸的干预作用
Influence of Oxidized Fish Oil on the Intestinal Health of Yellow Catfish (Pelteobagrus fulvidraco) and the Use of Arginine as an Intervention Measure
投稿时间:2016-08-09  修订日期:2016-12-17
DOI:10.11964/jfc.20160810507
中文关键词: 黄颡鱼  氧化鱼油  精氨酸  肠道  免疫指标  抗氧化指标
英文关键词: Yellow catfish  Oxidized fish oil  Arginine  Intestine  Immune  Antioxidant  Tissue structure
基金项目:(31402307)第一;国家自然科学基金
作者单位E-mail
卓丽欣 上海海洋大学水产与生命学院 1102267451@qq.com 
赵红霞 广东省畜禽育种与营养研究重点实验室
广东省农业科学院动物科学研究所 
 
黄燕华 广东省畜禽育种与营养研究重点实验室
广东省农业科学院动物科学研究所 
 
曹俊明 广东省畜禽育种与营养研究重点实验室
广东省农业科学院动物科学研究所 
junmcao@163.com 
王国霞 广东省畜禽育种与营养研究重点实验室
广东省农业科学院动物科学研究所 
 
陈 冰 广东省畜禽育种与营养研究重点实验室
广东省农业科学院动物科学研究所 
 
孙育平 广东省畜禽育种与营养研究重点实验室
广东省农业科学院动物科学研究所 
 
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中文摘要:
      [目的]本试验研究了饲料中添加氧化鱼油对黄颡鱼(Pelteobagrus fulvidraco)幼鱼肠道免疫、抗氧化和其它功能指标、组织结构的影响及添加精氨酸对其的干预作用。[方法]采用新鲜鱼油(FF):氧化鱼油(OF)按照(w/w)2.5:0、1.5:1.0和0.5:2.0配制3种饲料(FF,OF1,OF2),在此基础上分别添加0.48%精氨酸(Arg)盐酸盐配制3种精氨酸饲料(FFA,OFA1,OFA2)。选取初始重为4.41±0.05 g的黄颡鱼600尾,随机分为6组,每组4个重复,分别投喂6种试验饲料,饲养56 d。[结果]结果显示,在OF2组中,黄颡鱼肠道ACP、AKP活性和IL-6含量显著升高(P<0.05);与OF2组相比,OFA2组AKP活性和IL-6含量均显著下降(P<0.05)。在FF、OF1、OF2三组中,肠道SOD活性呈下降趋势,GSH-PX和CAT活性呈现上升趋势,T-AOC呈现先下降后上升的趋势,差异均不显著(P>0.05);在OF2组中,MDA含量显著升高(P<0.05);添加精氨酸后,除SOD活性有显著升高(P<0.05)外,其他抗氧化指标的组间均无显著性差异(P>0.05)。双因素方差分析显示,精氨酸对黄颡鱼肠道CAT活性的影响达到显著水平(P<0.05),饲料中氧化鱼油和精氨酸对黄颡鱼肠道GSH-PX活性的影响存在交互作用(P<0.05)。在FF、OF1、OF2三组中,肠道DAO活性呈现下降趋势,iNOS活性呈现上升趋势,但差异均不显著(P>0.05);与OF2组相比,OFA2组DAO和iNOS活性分别显著升高和下降(P<0.05);双因素方差分析显示,精氨酸对黄颡鱼肠道DAO、iNOS活性的影响分别达到显著水平(P<0.05)。在OF1组中,肠道皱壁高度、肌层厚度和杯状细胞数量均显著升高(P<0.05);与OF2组相比,OFA2组皱壁高度显著升高(P<0.05);与FF组相比,FFA组肌层厚度显著增加(P<0.05)。[结论]研究表明,在饲料中添加一定水平的氧化鱼油会抑制黄颡鱼幼鱼肠道免疫及抗氧化指标,损伤肠道组织结构,但添加一定量的精氨酸可以干预氧化鱼油对黄颡鱼幼鱼肠道免疫、抗氧化指标和组织结构的抑制作用。
英文摘要:
      (Objective ) This experiment was conducted to investigate the influence of dietary oxidized fish oil (OF) and the intervention of Arginine (Arg) on the intestinal health of juvenile yellow catfish (Pelteobagrus fulvidraco). (Method) A total of 600 healthy yellow catfish with an initial body weight of (4.41 ± 0.05) g were randomly divided into 6 groups with 4 replicates of 25 fish. During 56 d feeding trial, the fish were fed respectively six diets containing 2.5% fresh fish oil (FF), 1.5% FF + 1.0% OF (OF1), 0.5% FF + 2.0% OF (OF2), 2.5% FF + 0.48% Arg (FFA), 1.5% FF + 1.0% OF + 0.48% Arg (OFA1) , 0.5% FF + 12.% OF +0.48% Arg (OFA2). (Results) The results showed that the ACP, AKP activities and IL-6 content in intestine of yellow catfish were significantly higher in OF2 group (P<0.05). While addition of arginine to the oxidized fish oil diets, the AKP activity and IL-6 content in intestine of yellow catfish were significantly lower in OFA2 group (P<0.05). The SOD activity in intestine of yellow catfish showed a downward trend and the GSH-PX and CAT activities showed a reversed trend, but there was no significant differences among FF, OF1 and OF2 groups (P>0.05). The SOD activity was significantly higher in OFA2 group (P<0.05). The MDA content in intestine of yellow catfish were significantly higher in OF2 group (P<0.05). Dietary oxidized fish oil and arginine had a significant interaction on the GSH-PX activity in intestine of yellow catfish (P<0.05). The CAT activity in intestine was significantly decreased by the arginine (P<0.05). The DAO activity in intestine of yellow catfish showed a downward trend and the iNOS showed a raised trend , but there was no significant difference among FF, OF1 and OF2 groups (P>0.05). The DAO activity was significantly increased and iNOS significantly decreased in OFA2 group (P<0.05). The arginine had a significant impact on the DAO and iNOS activities in intestine of yellow catfish (P<0.05). The height of folds, the thickness of muscles and the number of goblet cells in foregut was significantly increased in OF1 group (P<0.05). In comparison with OF2 group, the height of folds was significantly increased in the OFA2 group (P<0.05). (Conclusion) The results suggest that dietary oxidized fish oil reduce the intestinal immune and total antioxidant capacity, and damage the structure in foregut of juvenile yellow catfish, but arginine supplementation can alleviate the inhibition induced by oxidized fish oil for intestinal immune, antioxidant capacity and tissue structure.
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