文章摘要
刘连为,周永东,陆化杰,刘必林,陈新军.西北太平洋鸢乌贼种群遗传结构[J].水产学报,2017,41(9):1355~1364
西北太平洋鸢乌贼种群遗传结构
Genetic structure of Sthenoteuthis oualaniensis population in the northwest Pacific Ocean
投稿时间:2016-09-05  修订日期:2017-02-07
DOI:10.11964/jfc.20160910527
中文关键词: 鸢乌贼  种群遗传结构  细胞色素b基因  西北太平洋
英文关键词: Sthenoteuthis oualaniensis  population genetic structure  Cytb  northwest Pacific Ocean
基金项目:大洋渔业资源可持续开发省部共建教育部重点实验室开放基金(A-0209-15-0503-10)
作者单位E-mail
刘连为 浙江省海洋水产研究所, 农业部重点渔场渔业资源科学观测实验站, 浙江省海洋渔业资源可持续利用技术研究重点实验室, 浙江 舟山 316021
浙江海洋大学海洋与渔业研究所, 浙江 舟山 316022 
 
周永东 浙江省海洋水产研究所, 农业部重点渔场渔业资源科学观测实验站, 浙江省海洋渔业资源可持续利用技术研究重点实验室, 浙江 舟山 316021
浙江海洋大学海洋与渔业研究所, 浙江 舟山 316022 
 
陆化杰 上海海洋大学海洋科学学院, 大洋渔业资源可持续开发省部共建教育部重点实验室, 上海 201306  
刘必林 上海海洋大学海洋科学学院, 大洋渔业资源可持续开发省部共建教育部重点实验室, 上海 201306  
陈新军 上海海洋大学海洋科学学院, 大洋渔业资源可持续开发省部共建教育部重点实验室, 上海 201306 xjchen@shou.edu.cn 
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中文摘要:
      为检测西北太平洋鸢乌贼种群遗传结构,采用线粒体DNA细胞色素b基因(Cytb)序列分析方法对鸢乌贼东海群体、南海群体与菲律宾海群体进行遗传变异分析。结果显示,①所有群体总的单倍型多样度与核苷酸多样度分别为0.982±0.006、0.012±0.006;菲律宾海群体对应的遗传多样度均最高,分别为0.973±0.014、0.015±0.008;南海群体与东海群体的单倍型多样度分别为0.959±0.026、0.943±0.031,核苷酸多样度均为0.006±0.003。3个地理群体均具有较高的遗传多样性水平。②分子方差分析结果显示,34.6%的遗传变异来自于群体间,群体间遗传分化极显著。两两群体间Fst分析表明,西北太平洋鸢乌贼群体间均具有极显著的遗传分化。构建的单倍型邻接系统树和最小跨度树显示,西北太平洋鸢乌贼群体存在明显的系统发育谱系结构(谱系A、B、C),3个谱系单倍型类群间也存在极显著的遗传分化(Fst=0.735~0.805)。③中性检验和核苷酸不配对分析结果均表明,谱系B可能经历过近期群体扩张事件,发生群体扩张的时间在10.3~12.5万年前。综合分析认为,西北太平洋鸢乌贼的种群遗传结构模式及系统发育地理格局模式是由其栖息地海洋环境与更新世气候变化共同塑造的。建议在渔业管理上将3个地理群体划分为3个独立的管理单元。
英文摘要:
      The purpleback flying squid Sthenoteuthis oualaniensis is widely distributed in tropical and subtropical areas of the Pacific Ocean and the Indian Ocean. In the northwest Pacific, the highest concentration is found in the South China Sea, while the waters of Ryukyu Islands and southwest Taiwan are regarded as fishing grounds of S. oualaniensis. To manage this squid and exploit it scientifically, it is important to investigate the genetic structure of its population. In this study, the genetic diversity of S. oualaniensis from the northwest Pacific (including the South China Sea, the East China Sea, and the Philippine Sea) was revealed, and sequence analysis of the mitochondrial DNA cytochrome b gene (Cytb) was used to assess its genetic structure. A total of 105 partial sequences of cytb were obtained, each 896 bp long, from which 72 haplotypes were defined. Haplotype diversities and nucleotide diversities for all geographical populations were 0.982±0.006 and 0.012±0.006, respectively, with corresponding maximum values of 0.973±0.014 and 0.015±0.008, respectively, in the population from the Philippine Sea. Haplotype diversities in populations from the South China Sea and the East China Sea were 0.959±0.026 and 0.943±0.031, respectively, while nucleotide diversities were 0.006±0.003 for both areas. Three populations from the South China Sea, the East China Sea, and the Philippine Sea were characterized by a higher haplotype diversity and higher nucleotide diversity. Analysis of molecular variance showed that 34.6% of genetic variance came from individuals among populations, and highly significant genetic differentiation was detected; a similar result was obtained from the fixation indices (Fst) among the three populations. The neighbor-joining haplotype tree and reduced median network analysis detected three distinct lineages (A, B, and C), and highly significant genetic differentiation was detected among the lineages (Fst=0.735-0.805). Neutrality tests and mismatch distribution analysis suggested that lineage B recently experienced a population expansion, around 103 000-125 000 years ago. In summary, the genetic structure and phylogeographic pattern of S. oualaniensis in the northwest Pacific appear to have been caused by ocean currents and climatic changes since the Pleistocene. The northwest Pacific S. oualaniensis should be considered as at least three stocks in their assessment and management.
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