文章摘要
邸军,张书环,黄君,杜浩,周勇,危起伟.中华鲟脑膜败血伊丽莎白菌的分离鉴定及药敏特性研究[J].水产学报,2018,42(1):120~130
中华鲟脑膜败血伊丽莎白菌的分离鉴定及药敏特性研究
Isolation and identification of Elizabethkingia meningoseptica from Chinese sturgeon (Acipenser sinensis) and its antibiotic sensitivity
投稿时间:2016-11-23  修订日期:2017-04-25
DOI:10.11964/jfc.20161110616
中文关键词: 中华鲟  脑膜败血伊丽莎白菌  分离鉴定  药敏试验  病理特征
英文关键词: Chinese sturgeon (Acipenser sinensis)  Elizabethkingia meningoseptica  isolation and identification  antibiotic susceptibility test  Pathologic characteristics
基金项目:国家重点基础研究规划项目
作者单位E-mail
邸军 西南大学 1092882119@qq.com 
张书环 长江水产研究所  
黄君 长江水产研究所  
杜浩 长江水产研究所  
周勇 长江水产研究所  
危起伟 长江水产研究所 weiqw@yfi.ac.cn 
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中文摘要:
      为了确定中华鲟养殖基地一尾死亡子二代中华鲟(Acipenser sinensis)的病原,利用传统病原菌分离方法,从濒死的中华鲟肝脏、脾脏和腹水中均分离到一株优势菌EM,采用16S rDNA基因序列分析、构建系统发育树并结合Biolog微生物自动分析系统进行鉴定,确定菌株EM为脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)。进一步对健康子二代达氏鲟进行人工感染试验确定其致病性;同时通过患病子二代中华鲟组织切片分析其病理特征;并对菌株EM进行药敏特性研究以期指导临床用药。结果显示,感染子二代中华鲟肝脏、脾脏和肾脏组织中铁血黄素沉积、实质性细胞空泡变性,溶血严重。菌株EM人工感染健康子二代达氏鲟,发现其对达氏鲟具有致病性,且从人工感染患病的达氏鲟肝脏中再次分离到相同的病原菌。药物敏感性试验表明,中华鲟脑膜败血伊丽莎白菌对米诺环素、克林霉素、克拉霉素、麦迪霉素4种药物敏感,对头孢噻肟和头孢西叮2种药物中度敏感,对其他29种药物不敏感,呈多重耐药性。本研究首次发现脑膜败血伊丽莎白菌可感染中华鲟,并对达氏鲟也具有致病性,初步研究了该病原菌的生物学特性、病理特征和药物敏感性,为中华鲟脑膜败血伊丽莎白菌的防治和中华鲟物种保护提供理论依据。
英文摘要:
      To define the potential pathogens causing a sick F2 Chinese sturgeon (Acipenser sinensis) in a hatchery of Chinese sturgeon, a bacterial strain EM was isolated from the liver; spleen and ascites of moribund F2 Chinese sturgeon by traditional method of pathogen isolation. The strain EM was identified as Elizabethkingia meningoseptica by means of the 16S rDNA sequence, constructing phylogenetic tree and Biolog Microbial Identification System. The pathogenicity of the strain EM was confirmed by the infection experiment in healthy F2 Dabry’s sturgeon (Acipenser dabryanus). Meanwhile, histopathological analysis was performed in the sick F2 Chinese sturgeon,and the antibiotic susceptibility of the strain EM was carried out to guide the clinically choosing medicine. The results showed that themosiderin deposition, ballooning degeneration and hemolysis severely in liver, spleen and kidney of the sick F2 Chinese sturgeon. The strain EM was cofirmed to be pathogenic to healthy Dabry’s sturgeon by infection experiment, the same bacterium could be recovered from these infected Dabry’s sturgeon.The susceptibility test to antibiotics demonstrated that the strain EM from sick Chinese sturgeon was susceptible to minocycline, clindamycin, clarithromycin,amd midecamycin; medium susceptible to cefotaxime and cefoxitin, but resistant to other 29 kinds of antibiotics, which implied EM strain was resistant to multiple antibiotics.In short, the study firstly shows that Elizabethkingia meningoseptica could be infected Chinese sturgeon, which is also known as a pathogen to Dabry’s sturgeon. Preliminary study on biological characteristics, antibiotic susceptibility of Elizabethkingia meningoseptica, pathological features of diesased F2 Chinese sturgeon, will provide theory basis for disease control and prevention on conservation of Chinese sturgeon.
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