文章摘要
徐敏杰,张佳鑫,黄根勇,张聪,成永旭,杨筱珍.L-色氨酸和褪黑激素对中华绒螯蟹(Eriocheir sinensis)血清血糖水平及肝胰腺抗氧化能力的影响[J].水产学报,2018,42(1):91~99
L-色氨酸和褪黑激素对中华绒螯蟹(Eriocheir sinensis)血清血糖水平及肝胰腺抗氧化能力的影响
Effects of L-tryptophan and melatonin for the serum glucose level and antioxidant capacity in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis)
投稿时间:2017-01-18  修订日期:2017-04-07
DOI:10.11964/jfc.20170110694
中文关键词: L-色氨酸  褪黑激素  中华绒螯蟹  血糖水平  抗氧化能力
英文关键词: L-tryptophan  melatonin  Eriocheir sinensis  glucose level  antioxidant capacity
基金项目:国家自然科学基金
作者单位E-mail
徐敏杰 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 1207169867@qq.com 
张佳鑫 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室  
黄根勇 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室  
张聪 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室  
成永旭 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室  
杨筱珍 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室 xzyang@shou.edu.cn 
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中文摘要:
      为探究L-色氨酸和褪黑激素(MT)对中华绒螯蟹血糖水平及肝胰腺抗氧化能力的影响。实验选取初始体质量为(16.58±2.20)g的雌性中华绒螯蟹,随机分成8组(L-色氨酸和MT处理各4组),实验周期均为30 d。(1)L-色氨酸饲料投喂实验4组:L-色氨酸所占饲料比重分别为0.36%(基础饲料即对照组)、0.47%、0.73%和1.05%(分别记为1、2、3和4组),30d后分别测定各组蟹的血糖水平及肝胰腺的T-AOC和SOD活性;(2)MT注射实验4组:每组分别在第1天和15天时注射生理盐水(即对照组,C组)和不同剂量的MT 10-6 mol/只(μM组)、10-9 mol/只(nM组)和10-12 mol/只(pM组)。分别于第1天和15天时,收集血淋巴和肝胰腺,分别测定血糖水平、T-AOC和SOD活性。于第30天时,再次收集血淋巴以测定血糖水平。结果显示:不同含量L-色氨酸对中华绒螯蟹血糖水平无明显影响,其血糖含量约为(4.62±0.20)mmol/L。此外,0.73% L-色氨酸的饲料能显著提高肝胰腺SOD活性(82.86±1.07)U/mL;注射MT仅在第1天时对中华绒螯蟹的血糖水平有促进作用,并呈剂量依赖性的方式升高,其中μM组蟹血糖高达(7.56±0.36)mmol/L,其值显著高于C、pM和nM组。此外注射MT在第1天时,对肝胰腺T-AOC和SOD活性也有明显的促进作用。其中,μM组蟹肝胰腺T-AOC和SOD活性均显著高于C、pM和nM组。综上所述,饲喂0.73% L-色氨酸的饲料能够提高中华绒螯蟹的抗氧化能力,但对血糖水平的影响不明显;注射10-6 mol/只MT可以在短时间内(1d)提高中华绒螯蟹的血糖水平和抗氧化能力。
英文摘要:
      In order to investigate the effects of L-tryptophan and melatonin for the serum glucose level and antioxidant capacity in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis). The female crab with the average body weight of (16.58±2.20)g were randomly allocated into 8 groups (the processing experiments of L-tryptophan and melatonin had 4 groups respectively). Each experimental period was 30 d. (1)The feeding experiment of L-tryptophan: crabs were fed with diet which added 4 levels of L- tryptophan, and the L-tryptophan account for the diet were 0.36% (basal diet namely control group), 0.47%, 0.73% and 1.05% (named diet 1, 2, 3 and 4, respectively). The serum glucose level and T-AOC and the capacity of SOD in the hepatopancreas were determined. (2)The injection experiment of melatonin: on the 1st and 15th day, crabs were injected with saline(control group namely C)and different doses of melatonin which were 10-6 mol/crab (μM), 10-9 mol/crab (nM) and 10-12 mol/crab (pM). Then the haemolymph and hepatopancreas were collected separately for the determination of the serum glucose level and T-AOC and the capacity of SOD in the hepatopancreas on the 1st and 15th day. In addition, we analyzed the serum glucose level on the 30th day. The results showed that different levels of L-tryptophan had no significant effect on serum glucose level, its value was approximately 4.62±0.20mmol/L. The diet which contained 0.73% L-tryptophan could significantly increase the capacity of SOD (82.86±1.07)U/mL. Injection of melatonin into crabs resulted in hyperglycemia in a dose-dependent manner on the 1st day. Among them, the serum glucose level of μM reached up to (7.56±0.36)mmol/L, which was significantly higher than other three groups. Moreover, the injection of MT played an important role in promoting the T-AOC and the capacity of SOD, but only occurred on the 1st day. Comparing with C, pM and nM, the the T-AOC and the capacity of SOD of μM was higher than them significantly. In summary, 0.73% L-tryptophan can improve the capacity of antioxidant of crabs, but the effects on the serum glucose level are not obvious. The injection of 10-6mol/crab melatonin can improve the serum glucose level and antioxidant capacity of Chinese mitten crab in short time (1 d).
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