文章摘要
谭青,王际英,李宝山,李学丽,郝甜甜,张利民.n-3/n-6 HUFA对大菱鲆幼鱼生长性能、全鱼脂肪酸组成和血清生化指标的影响[J].水产学报,2018,42(5):754~765
n-3/n-6 HUFA对大菱鲆幼鱼生长性能、全鱼脂肪酸组成和血清生化指标的影响
Effect of dietary n-3/n-6 HUFA on growth performance, fatty acid composition of whole fish and serum biochemical indices in turbot (Scophthalmus maximus)
投稿时间:2017-01-22  修订日期:2017-03-31
DOI:10.11964/jfc.20170110698
中文关键词: 大菱鲆  n-3/n-6 HUFA  生长  脂肪酸  血清生化指标
英文关键词: Scophthalmus maximus  n-3/n-6HUFA  growth  fatty acid  serum biochemical indices
基金项目:国家海洋生物产业-水生动物营养与饲料研发创新示范平台(201701002);山东省重点研发计划(2016GSF115005);山东省自然科学基金(ZR2015CQ023)
作者单位E-mail
谭青 上海海洋大学水产与生命学院, 上海 201306
山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006 
 
王际英 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
李宝山 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
李学丽 上海海洋大学水产与生命学院, 上海 201306
山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006 
 
郝甜甜 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
张利民 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006 zhanglimin@126.com 
摘要点击次数: 652
全文下载次数: 552
中文摘要:
      为探讨饲料中不同n-3/n-6高不饱和脂肪酸(HUFA)对大菱鲆幼鱼生长性能、全鱼脂肪酸组成和血液生化指标的影响,配制了6种不同n-3/n-6 HUFA(D1:29.54,D2:23.04,D3:18.97,D4:9.06,D5:6.86,D6:3.87)的实验饲料。以大菱鲆幼鱼(12.18±0.01) g为研究对象,在循环水养殖系统中开展了为期8周的养殖实验。实验共分6组,每组3个重复,每个重复35尾鱼。结果显示:饲料中n-3/n-6 HUFA对大菱鲆幼鱼的成活率(SR)无显著影响;增重率(WGR)、特定生长率(SGR)和蛋白质效率(PER)呈先上升后下降趋势且D6组的显著低于其他各组,D2组蛋白质效率显著高于其他各组。全鱼粗蛋白和灰分均呈先上升后下降趋势;D6组肌肉粗蛋白和灰分显著低于其他各组。全鱼ARA含量随着n-3/n-6 HUFA的下降呈上升趋势;全鱼中EPA、DHA、n-3/n-6 多不饱和脂肪酸(PUFA)和n-3/n-6 HUFA均随着饲料中n-3/n-6 HUFA的下降呈下降趋势。血清中酸性磷酸酶(ACP)和超氧化物歧化酶(SOD)随着n-3/n-6 HUFA的变化呈上升趋势;溶菌酶(LZM)和总抗氧化能力(T-AOC)呈先上升后下降的趋势,溶菌酶在D2组达到最大值,总抗氧化能力在D3组达到最大值。综上所述,饲料中n-3/n-6 HUFA在适宜范围(18.97~23.04)显著提高了实验鱼的生长性能和非特异性免疫力,改变了鱼体组织的常规成分和脂肪酸组成。
英文摘要:
      An 8-week feeding trial was conducted in a recirculated system to study the effect of dietary n-3/n-6 HUFA (D1:29.54, D2:23.04, D3:18.97, D4:9.06, D5:6.86, D6:3.87) on growth performance, the fatty acid composition of whole body and serum biochemical indices in turbot (Scophthalmus maximus). Six diets were formulated to feed six groups of juvenile turbot[mean initial weight (12.18±0.01) g], respectively. Each diet was randomly fed to triplicate groups of 35 fish per tank. Results showed that dietary n-3/n-6 HUFA had no significant effect on the survival rate(SR) of turbot. With the decreasing of the dietary n-3/n-6 fatty acid ratios, the weight gain rate (WGR), protein efficiency ratio (PER) and specific growth rate (SGR) increased first, then decreased. And WGR, PER and SGR of D6 group was significantly lower than other groups. PER of D2 group was significantly higher than other groups. Crude protein and ash of whole body increased at the beginning and then decreased. Crude protein and ash of muscle of D6 group was remarkably lower than the others. The fatty acid composition analysis showed that the concentration of ARA increased with decreasing of dietary n-3/n-6 HUFA, while EPA, DHA, n-3/n-6 PUFA and n-3/n-6 HUFA content followed the opposite pattern. Acid phosphatase (ACP) and superoxide dismutase (SOD) were increased among dietary treatments. Serum lysozyme (LZM) activity increased with reducing dietary n-3/n-6 HUFA from 29.54 to 23.04, and decreased with further decrease of n-3/n-6 HUFA from 23.04 to 3.87. Total antioxidant capacity (T-AOC) showed a similar trend with serum lysozyme activity with the highest value in the ratio of 18.97, significantly higher than that in the control and ratio of 3.87 groups. These results suggested that dietary n-3/n-6 HUFA, especially moderate n-3/n-6 HUFA (18.97~23.04), enhanced growth and immune response and modified the chemical composition of whole body and muscle of juvenile turbot.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看