文章摘要
郁维娜,戴文芳,陶震,熊金波.健康与患病凡纳滨对虾肠道菌群结构及功能差异研究[J].水产学报,2018,42(3):399~409
健康与患病凡纳滨对虾肠道菌群结构及功能差异研究
Characterizing the compositional and functional structures of intestinal microflora between healthy and diseased Litopenaeus vannamei
投稿时间:2017-03-05  修订日期:2017-04-25
DOI:10.11964/jfc.20170310737
中文关键词: 凡纳滨对虾  肠道菌群  功能组成  指示细菌科  功能冗余
英文关键词: Litopenaeus vannamei  intestinal microbiota  functional composition  indicator families  functional redundancy
基金项目:浙江省应用公益性项目(2016C32063);宁波市科技惠民项目(2017C10044)
作者单位E-mail
郁维娜 宁波大学海洋学院, 浙江 宁波 315211
浙江海洋高效健康养殖协同创新中心, 浙江 宁波 315211 
 
戴文芳 宁波大学海洋学院, 浙江 宁波 315211
浙江海洋高效健康养殖协同创新中心, 浙江 宁波 315211 
 
陶震 宁波大学海洋学院, 浙江 宁波 315211
浙江海洋高效健康养殖协同创新中心, 浙江 宁波 315211 
 
熊金波 宁波大学海洋学院, 浙江 宁波 315211
浙江海洋高效健康养殖协同创新中心, 浙江 宁波 315211 
xiongjinbo@nbu.edu.cn 
摘要点击次数: 1087
全文下载次数: 1196
中文摘要:
      为探究病害发生后健康与患病凡纳滨对虾肠道菌群结构和功能的差异,并筛选肠道指示菌群来评估宿主健康状况,评价凡纳滨对虾肠道菌落的功能冗余性。实验采集健康和患病凡纳滨对虾样品,通过Illumina高通量测序技术测定肠道菌群组成,并利用PICRUSt进行功能预测,以此比较健康与患病凡纳滨对虾肠道微生物的群落结构和功能差异,并预测功能与群落组成的相关性。结果显示,病害的发生伴随着肠道菌群结构的显著变化,而多样性无显著差异。与健康凡纳滨对虾肠道细菌组成相比,患病凡纳滨对虾肠道中放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)和疣微菌门(Verrucomicrobia)相对丰度降低,而γ-变形菌纲(Gammaproteobacteria)和拟杆菌门(Bacteroidetes)增加。同时,筛选出16个指示细菌科,能够很好地指示宿主健康状况。与健康组相比,患病凡纳滨对虾中参与弧菌侵染的过程显著增加,而溶酶体和过氧化物酶等免疫功能代谢过程显著减弱。此外,肠道微生物群落结构与功能组成呈显著正相关,表明凡纳滨对虾肠道菌群组成具有较低的功能冗余性。研究表明,健康与患病凡纳滨对虾肠道菌群结构存在显著差异,并且由细菌介导的功能随之发生改变,能够用指示微生物评估宿主健康状况。
英文摘要:
      To investigate the difference of the compositional and functional structures of the intestinal microflora between healthy and diseased Litopenaeus vannamei, we screened the intestinal bio-indicators to evaluate the health status of the host, and assess the functional redundancy of the intestinal microflora in response to disease. Based on the Illumina high-throughput sequencing technology, we compared the intestinal microflora between healthy and diseased L. vannamei, and analyzed the functional profiles through the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). In addition, we estimated the correlation between the intestinal microflora structures and predicted the functional compositions. The results showed that the occurrence of disease was associated with significant changes of the bacterial community structures and functional compositions, and there was no significant change in bacterial diversity. Compared with the intestinal microflora of the healthy L. vannamei, the relative abundances of Actinobacteria, Planctomycetes and Verrucomicrobia significantly decreased in the diseased L. vannamei, whereas, the Gammaproteobacteria and Bacteroidetes exhibited an opposite pattern. In addition, 16 sensitive indicator families were screened, and their occurrences can indicate the health status of the host very well. Furthermore, the abundance of pathways involved in Vibrio spp. infection significantly increased in the diseased L. vannamei; in contrast, the pathways for antibacterial and immunity, such as lysosome and peroxisome pathways, markedly decreased in diseased shrimp. Notably, there was a significant and positive association in compositional and functional composition between the gut microbiota community, indicating a low functional redundancy of the shrimp gut bacterial community. The occurrence of disease was associated with the change of the intestinal microflora, which subsequently disrupts the bacterial-mediated functions. Further, this study provides sensitive bio-indicators for evaluating the host health status.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看