文章摘要
崔益玮,李诗言,王珏,宋恭帅,王扬,戴志远,沈清.水产加工副产物中磷脂和鱼油的分离及脂质组学研究[J].水产学报,2017,41(6):827~835
水产加工副产物中磷脂和鱼油的分离及脂质组学研究
Separation of phospholipids and fish oil from fishery by-products followed by lipidomics study
投稿时间:2017-03-15  修订日期:2017-04-19
DOI:10.11964/jfc.20170310748
中文关键词: 鱼油  磷脂  水产加工副产物  气相色谱法  串联质谱法  脂质组学
英文关键词: fish oil  phospholipids  fishery by-products  gas chromatographic  tandem mass spectrometry  lipidomics
基金项目:国家自然科学基金(31601542);浙江省自然科学基金(LQ16C200001);浙江省重点研发项目(2017C03041)
作者单位E-mail
崔益玮 浙江工商大学海洋食品研究院, 浙江 杭州 310012  
李诗言 浙江工商大学海洋食品研究院, 浙江 杭州 310012
浙江省水产质量检测中心, 浙江 杭州 310023 
 
王珏 浙江工商大学海洋食品研究院, 浙江 杭州 310012  
宋恭帅 浙江工商大学海洋食品研究院, 浙江 杭州 310012  
王扬 浙江省水产质量检测中心, 浙江 杭州 310023  
戴志远 浙江工商大学海洋食品研究院, 浙江 杭州 310012  
沈清 浙江工商大学海洋食品研究院, 浙江 杭州 310012 leonqshen@163.com 
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中文摘要:
      为更好地对水产加工副产物进行加工利用,本实验建立了从水产加工副产物中同时提取制备磷脂和鱼油的方法,并分别利用多维度串联质谱鸟枪法和气相色谱法对样品的磷脂和鱼油的脂肪酸化合物进行脂质组学研究。利用改进Folch法和丙酮沉淀法制备得到副产物中的磷脂和鱼油,比较了5种不同萃取液的提取效率,其中CH2Cl2-MeOH (1∶2,V/V)混合液同时提取磷脂和鱼油效果最佳,提取率分别为0.97%和28.05%。鱼油经甲酯化反应,以正己烷为溶剂直接进样,利用气相色谱法分析脂肪酸甲酯成分,磷脂样品采用流动注射直接进样,经三重四级杆质谱母离子扫描(PreIS)和中性质量丢失扫描(NLS)对磷脂分子实现源内分离鉴定和定量分析。结果显示,鱼油中主要含有油酸、亚油酸、棕榈酸等23种脂肪酸,其中不饱和脂肪酸占75.97%;磷脂样品中共检出磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰丝氨酸4类共69种磷脂分子,并测出了许多含有DHA、EPA链的磷脂,如16∶0/20∶5、16∶0/22∶6、18∶1/22∶6、18∶0/22∶6、20∶0/22∶6等。研究表明,该方法能同时提取磷脂和鱼油,并且前处理简单、提取效率高,能够实现对资源的合理利用。
英文摘要:
      For full utilization of the fishery wastes, the aim of this study was to establish a method for extracting the phospholipids and fish oil simultaneously from wastes, followed by the analysis of phospholipid and fatty acid composition on the basis of multi-dimensional tandem mass spectrometry and gas chromatography. Phospholipids and fish oil were extracted simultaneously by modified Folch method and precipitation method with acetone. The extracting efficiency of five different extracts was compared, and the mixture of CH2Cl2-MeOH (1∶2, V/V) performed best, whose extracting rates of phospholipids and fish oil were 0.97%和28.05%, respectively. Afterwards, the obtained fish oil was esterified and analyzed by gas chromatography, and the obtained phospholipids were injected directly by a syringe pump and identified and quantified by tandem mass spectrometry in the precursor-ion scan (PreIS) and neutral-loss scan (NLS) modes. The results show that fish oil contains 23 kinds of fatty acids, including oleic acid, linoleic acid, palmitic acid, etc. The content of unsaturated fatty acid is 75.97%. There are four classes (phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl serine) and 69 kinds of phospholipid molecules were detected in the phospholipid extract. Phospholipids with at least one eicosapentaenoic acyl/docosahexaenoic acyl chain were frequently found, such as 16:0/20:5, 16:0/22:6, 18:1/22:6, 18:0/22:6, 20:0/22:6, etc. In conclusion, this method can efficiently extract phospholipids and fish oil simultaneously. It is simple, fast, and friendly to the environment.
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