文章摘要
王永红,张建设,曾霖.β-葡聚糖对低氧胁迫下大黄鱼幼鱼的保护作用及其机理[J].水产学报,2018,42(6):828~837
β-葡聚糖对低氧胁迫下大黄鱼幼鱼的保护作用及其机理
β-glucan decreases intensity of hypoxia-induced oxidative stress in large yellow croaker (Larimichthys crocea) and its corresponding mechanisms
投稿时间:2017-06-16  修订日期:2017-10-10
DOI:10.11964/jfc.20170610877
中文关键词: 大黄鱼  β-葡聚糖  氧化应激  Keap1-Nrf 2信号通路
英文关键词: Larimichthys crocea  β-glucan  oxidative stress  Keap1-Nrf 2 signaling pathway
基金项目:浙江省自然科学基金(LY18D060008)
作者单位E-mail
王永红 浙江海洋大学国家海洋设施养殖工程技术研究中心, 浙江 舟山 316000  
张建设 浙江海洋大学国家海洋设施养殖工程技术研究中心, 浙江 舟山 316000  
曾霖 浙江海洋大学国家海洋设施养殖工程技术研究中心, 浙江 舟山 316000 zenglin615@126.com 
摘要点击次数: 897
全文下载次数: 1763
中文摘要:
      为探讨β-葡聚糖对低氧胁迫下大黄鱼幼鱼肝脏中丙二醛(MDA)、抗氧化酶(Cu/Zn-SOD、CAT、GPx和GR)活性和基因水平及核转录因子Keap1和Nrf 2基因水平的影响,将平均体质量为(76.53±0.74) g的大黄鱼幼鱼腹腔注射浓度为0或5 mg/kg体质量的β-葡聚糖0.1 mL,再暴露在溶解氧浓度为1.5或7.0 mg/L的水体中24 h。结果显示,β-葡聚糖在常氧环境下对MDA不产生影响,但β-葡聚糖可减少低氧胁迫下大黄鱼幼鱼MDA的含量,表明β-葡聚糖可缓解低氧胁迫对大黄鱼幼鱼的氧化损伤。Nrf 2基因水平与抗氧化酶基因水平成正相关,表明Nrf 2参与了低氧胁迫下大黄鱼幼鱼抗氧化反应。Nrf 2基因水平与Keap1基因水平成负相关,表明Keap1在调节Nrf 2参与抗氧化反应方面发挥重要作用。研究表明,β-葡聚糖可缓解大黄鱼幼鱼低氧胁迫诱导的氧化损伤,核转录因子Nrf 2在这一过程中发挥重要作用。
英文摘要:
      The aim of the present study was to evaluate the effects of β-glucan on acute hypoxia-induced oxidative stress by determining lipid peroxidation, activities and mRNA levels of antioxidant enzymes (Cu/Zn-SOD, CAT, GPx and GR), and gene expressions of the Keap1-Nrf 2 signaling molecules in the liver of large yellow croaker Larimichthys crocea. Fish were injected with β-glucan at a dose of 0 or 5 mg/kg body weight on 6, 4 and 2 days before being exposed to 1.5 and 7.0 DO mg/L for 24 h. The results showed that normoxia with β-glucan had no effect on lipid peroxidation compared with the normoxic control, and hypoxic stress with β-glucan remarkably reduced lipid peroxidation during the whole experiment compared with hypoxic stress, indicating that β-glucan could ameliorate hypoxia-induced lipid peroxidation. Obtained results also showed a coordinated transcriptional regulation of antioxidant genes, suggesting that Nrf 2 was required for regulating these genes. Furthermore, a negative relationship between the mRNA levels of Nrf 2 and Keap1 indicated that Keap1 plays an important role in switching off the Nrf 2 response. In conculsion, β-glucan could alleviate acute hypoxia-induced oxidative stress in large yellow croaker, emphasizing a central role of transcription factor Nrf 2 in the process.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看