文章摘要
李阳,薛素燕,李加琦,沈淑芳,陈琼琳,蒋增杰,方建光,毛玉泽.Cu2+胁迫对魁蚶生理生化和组织结构的影响[J].水产学报,2018,42(10):1531~1540
Cu2+胁迫对魁蚶生理生化和组织结构的影响
Effect of Cu2+ stress on physiology biochemistry and histopathological structure of Scapharca broughtonii
投稿时间:2017-08-14  修订日期:2018-01-31
DOI:10.11964/jfc.20170810928
中文关键词: 魁蚶  Cu2+胁迫  生理生化  组织结构
英文关键词: Scapharca broughtonii  Cu2+ stress  physiological metabolism  histopathological structure
基金项目:国家基金委-山东省联合基金(U1606404);中国水产科学研究院黄海水产研究所基本科研业务费专项(20603022017002,20603022017010);国家自然科学基金(41761134052);国家贝类产业技术体系专项(CARS-49)
作者单位E-mail
李阳 上海海洋大学水产与生命学院, 上海 201306
中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071 
 
薛素燕 中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071
青岛海洋科学与技术国家实验室, 海洋生态与环境科学功能实验室, 山东 青岛 266071 
 
李加琦 中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071
青岛海洋科学与技术国家实验室, 海洋生态与环境科学功能实验室, 山东 青岛 266071 
 
沈淑芳 上海海洋大学水产与生命学院, 上海 201306
中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071 
 
陈琼琳 上海海洋大学水产与生命学院, 上海 201306
中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071 
 
蒋增杰 中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071  
方建光 中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071  
毛玉泽 中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东省渔业资源与生态环境重点实验室, 山东 青岛 266071
青岛海洋科学与技术国家实验室, 海洋生态与环境科学功能实验室, 山东 青岛 266071 
maoyz@ysfri.ac.cn 
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中文摘要:
      采用生物毒性测试的方法,研究了持续暴露96 h不同浓度Cu2+胁迫对魁蚶生理代谢、组织结构及酶活性的影响。实验设置0.01、0.05、0.10、0.50和1.00 mg/L组5个浓度梯度,不加Cu2+的正常海水作为对照。结果显示,Cu2+胁迫对魁蚶耗氧率(OR)、排氨率(NR)、氧氮比(O∶N)均有显著影响,并且与暴露时间和浓度有关。初次暴露时,OR,NR和O∶N都迅速下降,最低值都出现在1.00 mg/L浓度组,分别为(0.005±0.001) mg/(g·h)、(0.5±0.05)μmol/(g·h)、0.7±0.1,仅为对照组的1%、29%和3%。暴露72 h内Cu2+胁迫均使魁蚶代谢率不同程度下降,之后不同处理组出现差异分化。暴露96 h时,魁蚶对低浓度Cu2+暴露表现出适应,0.01 mg/L处理组呼吸代谢恢复至正常水平,O∶N与对照组无差异,组织结构也未见明显损伤;浓度超过0.05 mg/L处理组魁蚶生理代谢及组织结构受显著影响,O∶N大多降至9以下,出现鳃丝受损和组织结构散乱等明显损伤;0.10 mg/L处理组魁蚶体内ACP和ALP活性在鳃组织中增强,肝脏中受到抑制,而GPX和GST活性增强。研究表明持续暴露96 h Cu2+浓度≥0.05 mg/L环境显著影响魁蚶生理代谢及组织结构,0.10 mg/L Cu2+浓度显著影响魁蚶组织中ACP、ALP、GPX和GST的活性。研究结果为认知魁蚶等滩涂贝类对Cu2+胁迫的响应机制提供基础数据,为预防滩涂潜在重金属污染风险及生物修复提供参考。
英文摘要:
      The stressful effect of Cu2+ with exposure duration for 96 hours on physiology metabolism, histopathological structure and enzyme activity of ark shell Scapharca broughtonii was studied by the methods of biological toxicity test. The incubating concentrations of Cu2+ were set at (0.01, 0.05, 0.10, 0.50, 1.00) mg/L and a control group was set with no Cu2+ stress (normal seawater). Results showed that incubating concentration and exposure duration both had significant influence on physiological indices of S. broughtonii, including oxygen consumption rates (OR), ammonia excretion rates (NR) and ratios of O to N (O∶N). The OR, NR and O∶N all decreased sharply after initial exposure to the Cu2+ stress. The minimum values for OR, NR and O∶N were found to be (0.005±0.001) mg/(g·h), (0.5±0.05) μmol/(g·h), 0.7±0.1 with the 1.00 mg/L treatment at the end of the experiment, which equaled 1%, 29%, 3% of control groups, respectively. Generally, apparently reduced metabolic rate of S. broughtonii individuals exposed to Cu2+ stress was observed within 72 h. After 72 h, each group with different Cu2+ concentration behaves differently. After being incubated at 0.01 mg/L for 96 h, the S. broughtonii seemed to be acclimated to Cu2+ stress. Metabolic rates of the animals were restored to the control level, while O∶N was not different from the control groups and no detectable histopathologic damage was found. The physiological metabolism and histopathological structure of individuals under concentrations that higher than 0.05 mg/L Cu2+ were significantly affected after 96 h, while most of the O∶N in all the experimental groups were below 9 and histopathologic damage such as gills damage and tissues messy structure were found. The ACP and ALP activities of individuals exposed to 0.10 mg/L Cu2+ increased in gill and decreased in hepatopancreas, while GPX and GST increased in both tissues. Our results demonstrated that incubating concentration (≥0.05 mg/L) after 96 h exposure significantly affect physiological metabolism and histopathological structure of the ark shell. ACP, ALP, GPX and GST activities of individuals incubated at 0.10 mg/L were significantly affected. Our results provide the basic data for studying the response mechanism of S. broughtonii and other coastal shellfishes to Cu2+ stress, and referential data for the prevention and biological reparation of potential heavy metal pollution risk in coast area.
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