文章摘要
杨华田,熊玉琴,杨锐.高温下芽孢杆菌对坛紫菜生长及生理的影响[J].水产学报,2018,42(7):1009~1018
高温下芽孢杆菌对坛紫菜生长及生理的影响
Effects of Bacillus sp. on Pyropia haitanensis at high temperature
投稿时间:2017-08-21  修订日期:2018-01-09
DOI:10.11964/jfc.20170810933
中文关键词: 坛紫菜  芽孢杆菌  藻际  功能
英文关键词: Pyropia haitanensis  Bacillus  phycosphere  function
基金项目:国家自然科学基金(31772871);浙江省重大科技专项(2016C02055-6B);现代农业产业技术体系专项(CARS-50);“水产”浙江省重中之重学科开放基金(xkzsc1509)
作者单位E-mail
杨华田 宁波大学海洋学院, 浙江省海洋生物重点实验室, 浙江 宁波 315211  
熊玉琴 宁波大学海洋学院, 浙江省海洋生物重点实验室, 浙江 宁波 315211  
杨锐 宁波大学海洋学院, 浙江省海洋生物重点实验室, 浙江 宁波 315211 yangrui@nbu.edu.cn 
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中文摘要:
      坛紫菜是我国东海区栽培的重要经济海藻,其生长适温为20 ℃,极限高温为29 ℃。高温和病原菌均会导致坛紫菜生长受抑、藻体病烂。为研究高温下藻际微生物对坛紫菜生长的影响,本文以坛紫菜叶状体和藻际芽孢杆菌(实验证明该菌株在20 ℃下为紫菜的益生菌)为材料,检测了在28 ℃下藻际芽孢杆菌对坛紫菜相对生长率(RGR),抗氧化酶(SOD、POD)活性,可溶性蛋白、游离脯氨酸(Pro)、丙二醛(MDA)和藻胆蛋白含量等生理指标的影响。结果显示,28 ℃下,在实验初始阶段,菌藻共培养组坛紫菜比纯培养对照组有较高的RGR,但随着培养时间的延长,两组的RGR无显著性差异;4 d后两组坛紫菜均出现腐烂,菌藻共培养组的藻体腐烂程度更为剧烈;培养24 h后,菌藻共培养组的SOD、POD活性和MDA水平均显著高于对照组,而渗透调节物质可溶性蛋白和Pro显著低于对照组;共培养组中,坛紫菜的藻胆蛋白含量均显著高于对照组。该研究表明,28 ℃下,虽然在短期内藻际芽孢杆菌会促进坛紫菜的生长,但随着培养时间的延长反而不利于坛紫菜的生长,且加剧活性氧和渗透压胁迫,表明该菌的生态功能因环境变化而发生了改变。
英文摘要:
      Pyropia haitanensis is an important economic seaweed cultivated in the East China Sea. Its optimum growth temperature is about 20℃, and the extremely high temperature is about 29℃. P. haitanensis is vulnerable to disease due to high temperature or hostile epiphytic bacterial flora in the phycosphere. In this study, the thalli of P. haitanensis were co-cultured with Bacillus sp. WPySW2 which was proved to be probiotic to Pyropia at 20℃. However, the experiment was conducted at 28℃, an extremely high temperature to Pyropia species. The physiological indexes such as relative growth rate (RGR), activities of antioxidant enzymes (SOD and POD), contents of soluble proteins, free proline (Pro), malondialdehyde (MDA), and phycobiliproteins (phycorythrin, phycocyanin, and allophycocyanin) were detected. The results showed that:at 28℃, the RGR of co-cultured P. haitanensis was higher than the control at the beginning, while the difference became less significant when the co-culture process continued. Both the control and co-cultured samples rotted 4 days later, and the conditions of co-culture groups were worse. After co-culturing for 24 h, the activities of SOD and POD, and the content of MDA in co-culture samples were higher than the control. The contents of soluble proteins and free Pro were much lower than those in the control. The contents of phycobiliproteins in co-cultured groups were higher than the control. It revealed that at the high temperature such as 28℃, Bacillus sp. WPySW2 could promote the growth of P. haitanensis at the beginning of co-culture, however, when the co-culture process continued, the bacteria inhibited the growth and the physiological conditions of the algae. The results indicated the phycospheric bacteria could change the ecological functions when the environment changed.
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