文章摘要
蒋晨毓,邱伟强,贠三月,陈舜胜.鳙和棕点石斑鱼冷藏过程中ATP关联化合物的变化[J].水产学报,2018,42(6):975~983
鳙和棕点石斑鱼冷藏过程中ATP关联化合物的变化
Degradation regularity of ATP-related compounds in Aristichys nobilis and Epinephelus fuscoguttatus during storage
投稿时间:2017-11-13  修订日期:2018-01-08
DOI:10.11964/jfc.20171111049
中文关键词:   棕点石斑鱼  高效液相色谱  ATP关联产物  贮藏
英文关键词: Aristichys nobilis  Epinephelus fuscoguttatus  HPLC  ATP-related compounds  storage
基金项目:国家自然科学基金(31471685);上海海洋大学博士启动基金(A2-0203-00-100340);上海市高校知识服务平台项目(ZF1206)
作者单位E-mail
蒋晨毓 上海海洋大学食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海海洋大学, 上海 201306 
 
邱伟强 上海海洋大学食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海海洋大学, 上海 201306 
 
贠三月 上海海洋大学食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海海洋大学, 上海 201306 
 
陈舜胜 上海海洋大学食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海海洋大学, 上海 201306 
sschen@shou.edu.cn 
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中文摘要:
      为分析鳙和棕点石斑鱼贮藏过程中ATP关联产物含量的变化,本研究以高氯酸溶液作为蛋白沉淀剂,运用高效液相色谱法(HPLC),分析(4±1) ℃和(0±1) ℃贮藏条件下鳙和棕点石斑鱼体内6种ATP关联化合物含量的变化。结果显示,(4±1) ℃条件下,2种鱼体内的ATP都快速降解;ADP、AMP含量则始终较低;IMP含量呈先快速上升后缓慢下降的趋势;鳙体内HxR含量相对较高,棕点石斑鱼体内HxR含量较少;Hx在4 d后即开始大量生成。在(0±1) ℃条件下,2种鱼肉中ATP同样很快降解,ADP、AMP含量少;IMP降解速率变缓;2种鱼中HxR生成和分解速率皆变缓;Hx生成速率较为平稳。鳙以IMP、HxR为限速步骤,棕点石斑鱼以IMP为限速步骤。研究表明,不同的贮藏温度对ATP的降解影响较小,但对其关联产物的变化速率有较大影响,且不同鱼种间ATP关联产物降解规律也有所不同。
英文摘要:
      The changes of ATP-related compounds in Aristichys nobilis and Epinephelus fuscoguttatus were studied during the storage at (4±1)℃ and (0±1)℃. Hyperchloric acid was used to precipitate proteins, 6 ATP-related compounds were analysed by high performance liquid chromatography (HPLC). Results showed that ATP in both fishes was rapidly degraded at (4±1)℃; ADP, AMP contents were always low; IMP content increased rapidly, then slowly decreased;the content of HxR in A. nobilis was relatively high, however, the content of HxR in E. fuscoguttatus was less; Hx began to largely generate after 4 days; at (0±1)℃, ATP in both fishes was also degraded quickly and ADP and AMP contents were low; IMP degradation rate became slow; the rate of formation and decomposition of HxR in both fishes was slowed down; Hx generation rate was stable. IMP and HxR were rate-limiting steps in A. nobilis, while IMP was rate-limiting step in E. fuscoguttatus. Different storage temperatures had little effect on ATP degradation, but would affect change rate of its associated compounds, and the degradation of ATP-related compounds in different species was also different.
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