文章摘要
李聪,成永旭,管勤壮,奚业文,李嘉尧.用稳定性同位素技术分析稻—虾系统中不同“碳/氮”投喂方式对克氏原螯虾食性的影响[J].水产学报,2018,42(11):1778~1786
用稳定性同位素技术分析稻—虾系统中不同“碳/氮”投喂方式对克氏原螯虾食性的影响
Using stable isotopes to estimate the effects of different carbon/nitrogen feeds on feeding habit of Procambarus clarkii in the rice-crayfish co-culture system
投稿时间:2017-12-29  修订日期:2018-03-27
DOI:10.11964/jfc.20171211115
中文关键词: 克氏原螯虾  稻-虾共作  C/N  稳定性同位素技术  食性分析
英文关键词: Procambarus clarkii  rice-crayfish co-culture  C/N  stable isotopes  diets analysis
基金项目:上海市科委农业领域科技支撑项目(15391912100);江苏省渔业科技类项目(D2017-1-1);上海高校水产学高峰学科建设专项(2015-62-0908)
作者单位E-mail
李聪 上海海洋大学, 农业部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学, 水产动物遗传育种中心上海市协同创新中心, 上海 201306 
 
成永旭 上海海洋大学, 农业部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学, 水产动物遗传育种中心上海市协同创新中心, 上海 201306 
 
管勤壮 上海海洋大学, 农业部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学, 水产动物遗传育种中心上海市协同创新中心, 上海 201306 
 
奚业文 安徽省水产技术推广总站, 安徽 合肥 230601  
李嘉尧 上海海洋大学, 农业部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学, 水产动物遗传育种中心上海市协同创新中心, 上海 201306 
jy-li@shou.edu.cn 
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中文摘要:
      为改善克氏原螯虾在稻—虾共作模式下对稻田中丰富自然资源的利用效率,本研究通过在饲料中添加碳源(麸皮、葡萄糖)调节饲料“碳/氮”(C/N)分别为8,12,16三个组别进行投喂,利用稳定性同位素技术分析各组克氏原螯虾的食性。结果显示,①不同C/N投喂组中不同规格成虾肌肉中的δ13C和δ15N值均较本底显著降低,成虾肌肉的δ13C值随投喂饲料C/N的增加而下降,B组不同规格成虾肌肉的δ15N值均较其他两组高。同一C/N投喂处理组中,1级小规格成虾肌肉的δ13C和δ15N值均为最高。②通过食性分析,组A中不同规格的克氏原螯虾的第一食物来源均为饲料,占比38.03%~44.17%,组B中饲料仍为不同规格克氏原螯虾的第一食物来源,但是占比下降为22.13%~25.35%,组C中1级小规格成虾中有机碎屑为第一食物来源,占19.93%,2级中规格成虾及3级大规格成虾的第一食物来源饲料进一步下降为18.85%和19.40%,组B及组C中浮游动物、藻类、喜旱莲子草等天然饵料食物来源占比均较组A显著增加。研究表明,通过优化C/N的投喂模式,可以提高克氏原螯虾对于稻田天然饵料的利用率,避免饲料的过度使用和浪费,有利于实现对稻田资源的合理利用。
英文摘要:
      In the rice-crayfish co-culture system, natural resources and primary productivity of rice fields are abundant, which provides abundant natural baits for Procambarus clarkii. At present, there is no report about the utilization of rice fields with P. clarkii. In this paper, C/N was increased by adding carbon source to feed, which makes nitrogen utilization increase and enriches the density of natural baits in rice fields. We designed three feeding modes as group A (formulated diet C/N: 8), group B (formulated diet and bran C/N: 12) and group C (formulated diet, bran and glucose C/N: 16). Stable isotope techniques were used for analyzing the feeding habits of P. clarkii. The results showed that: ①The δ13C and δ15N values of muscles in different dietary C/N groups were significantly lower than the juvenile P. clarkii muscle, and the δ13C values of adult P. clarkii muscle decreased with the increase of C/N. While the δ15N values of P. clarkii muscle in group B with different sizes were higher than the other two groups. In the same C/N treatment, the δ13C and δ15N value of crayfish muscle of a small size crayfish were the highest. ②According to the analysis of diet, formulated feed was the first food source of P. clarkii with different sizes in group A, accounting for 38.03%-44.17%. Formulated feed was still the first contribution of crayfish diet with different sizes in group B but dropped to 22.13%-25.35%. In group C, the organic debris for the diet of small size P. clarkii accounted for 19.93% was the first food source. Formulated feed which was the first food source of middle and large size P. clarkii declined further to 18.85% and 19.4% respectively. The natural food proportions including zooplankton, algae and Alternanthera philoxeroides were significantly increased in groups B and C compared with group A. The results indicate that by increasing the C/N of feed of rice-crayfish co-culture system, the utilization rate of natural food in the rice fields can be increased, and the excessive use and waste of the feed can be avoided, and it is beneficial to realize the rational utilization of rice fields resources.
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